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The map label for this gene is rpoE [H]

Identifier: 116622248

GI number: 116622248

Start: 3982909

End: 3983463

Strand: Reverse

Name: rpoE [H]

Synonym: Acid_3141

Alternate gene names: 116622248

Gene position: 3983463-3982909 (Counterclockwise)

Preceding gene: 116622249

Following gene: 116622247

Centisome position: 39.97

GC content: 60.36

Gene sequence:

>555_bases
ATGCTCTTCGCCAACAAGCCGCGGATGAACGAATTCGAGGCGGCGGCACTCCCGCACCTCAATGACATCTATCGCACCGC
CGCCCGCGTTCTCGGCGACAGGGCGCGCGCCGACGACGTGGTTCAGGACGTCTACCTCCAGGCCTGGAAGTCCTTCGACA
AGTTCGAGCCCGGCACCAACTGCCGCGCCTGGCTTTTCAAGATCCTCTTCCACACCGTGAATCACTACCGCCGCAAGTGG
CTGAATATTCGCATGGTCAAAGAGAGTGAAGAGGTCCTCCGAACCGCCGCCGCCGATCCGCCAGTCCCCGAGCAGATCAC
CGACCAGCATCTTCTCACGGCGCTCGACCAGGTGCCGCAGGATTTCCGCAGCGTTGTACTGCTGGTGGATGTCGAAGAGT
TCTCATATAAAGAGGCGTCGGCGATTTTGAGTGTACCGATCGGCACCATCATGTCGCGCCTGAGCCGCGGGCGAAAACTG
CTCCGCGAACGGCTCGCCGAAATGGCACGGCAATACGGAATCTTACGTGATCCTCAAGAAGGACGGAGCGCATGA

Upstream 100 bases:

>100_bases
GCGAAATCGCCTTCCAGTTCATGCGCCGTTAATATACCGCCTGCGGGAATATTCTGGGAATATTCTGGTGGATAGCCCGG
ATATACCTGTGAACAGGCCG

Downstream 100 bases:

>100_bases
CTCGACATCAGTTTGGAGAAGGCGCTTGCGAAAAAACGCGCCGCTACATGGACGCCTACCTCAGCAACGAACTGCTGGTG
GAGACTACACACGACGTTCT

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLFANKPRMNEFEAAALPHLNDIYRTAARVLGDRARADDVVQDVYLQAWKSFDKFEPGTNCRAWLFKILFHTVNHYRRKW
LNIRMVKESEEVLRTAAADPPVPEQITDQHLLTALDQVPQDFRSVVLLVDVEEFSYKEASAILSVPIGTIMSRLSRGRKL
LRERLAEMARQYGILRDPQEGRSA

Sequences:

>Translated_184_residues
MLFANKPRMNEFEAAALPHLNDIYRTAARVLGDRARADDVVQDVYLQAWKSFDKFEPGTNCRAWLFKILFHTVNHYRRKW
LNIRMVKESEEVLRTAAADPPVPEQITDQHLLTALDQVPQDFRSVVLLVDVEEFSYKEASAILSVPIGTIMSRLSRGRKL
LRERLAEMARQYGILRDPQEGRSA
>Mature_184_residues
MLFANKPRMNEFEAAALPHLNDIYRTAARVLGDRARADDVVQDVYLQAWKSFDKFEPGTNCRAWLFKILFHTVNHYRRKW
LNIRMVKESEEVLRTAAADPPVPEQITDQHLLTALDQVPQDFRSVVLLVDVEEFSYKEASAILSVPIGTIMSRLSRGRKL
LRERLAEMARQYGILRDPQEGRSA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=144, Percent_Identity=23.6111111111111, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21255; Mature: 21255

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFANKPRMNEFEAAALPHLNDIYRTAARVLGDRARADDVVQDVYLQAWKSFDKFEPGTN
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CRAWLFKILFHTVNHYRRKWLNIRMVKESEEVLRTAAADPPVPEQITDQHLLTALDQVPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
DFRSVVLLVDVEEFSYKEASAILSVPIGTIMSRLSRGRKLLRERLAEMARQYGILRDPQE
HHHHEEHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GRSA
CCCC
>Mature Secondary Structure
MLFANKPRMNEFEAAALPHLNDIYRTAARVLGDRARADDVVQDVYLQAWKSFDKFEPGTN
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CRAWLFKILFHTVNHYRRKWLNIRMVKESEEVLRTAAADPPVPEQITDQHLLTALDQVPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
DFRSVVLLVDVEEFSYKEASAILSVPIGTIMSRLSRGRKLLRERLAEMARQYGILRDPQE
HHHHEEHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GRSA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]