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The map label for this gene is fdnH [H]

Identifier: 116622139

GI number: 116622139

Start: 3826251

End: 3827111

Strand: Reverse

Name: fdnH [H]

Synonym: Acid_3027

Alternate gene names: 116622139

Gene position: 3827111-3826251 (Counterclockwise)

Preceding gene: 116622142

Following gene: 116622138

Centisome position: 38.4

GC content: 62.25

Gene sequence:

>861_bases
ATGCGCGCATTAACGGCCGCCGAAGCGCATCACGTGGAAATGAGAGCCGCGCGCGACGATACCGCCAACCGCACGGTCCG
CCGAGGATTCTTCACCGATACCACGCTCTGCATCGGCTGCAAGGCCTGCGAGGTCGCCTGCAAGCAGTGGAACCAACTGC
CGGACGATGGCTTTGTTTTCACGGGAATGTCGTACGACAACACCGTGGCGCTGGGCGCATCCACGTGGAGGCACGTAGCA
TTCATCGAGCGTCCGGTGGCCCTTTCAACGCAGGACGGCGGCGCGTTCTCCTGGCTCTTCTCCTCCGATGTGTGCAAGCA
CTGCGCGCGCGCCGGATGCCTGGAGTCCTGTCCAACCGGCGCTATCATTCGCACCGAGTTCGACAGCGTCTACGTGCAGC
CCGACATCTGCAACGGCTGCGGCTACTGCGTGATCAACTGCCCGTTCGGAGTCATCGACCGAAGTCCGGACGACGGTCGC
GCCTGGAAGTGCACCTTGTGCTACGACCGTATGAAGTTCGATATGGAGCCGGCGTGCGCGAAGGCGTGCCCGACGGACTC
GATCCAGTATGGCGATTTGGACGAGCTACTCAATGCCGCGCAGCGGCGCGTTGCGAAACTGCACGAAAACGGCATGCAAG
AGGCCTACCTCTACGGAGCCGATGCCGCAAGCCAGCCGGGAACGGAGGGCTTGAATGCGTTCTTTCTGTTGGTGGACAGG
CCCGAGGTCTACAATCTTCCCCCGAATCCCGTGGTTCCAACCAAGACGGTGAGAGAGACGTGGCGATCATTCGCATGGAC
AGTCGCGGGACTCGCCGCAGTTGCCGTCGGGTCCGTGTTCAGCGCGAAAGGAGTGCGCTGA

Upstream 100 bases:

>100_bases
GCCCGACCGCCAAGCCGAGCGCGATCTGCCGGAGCTCGAACGCAAGCCCGATACGGGCCACGGACACAAAACCGAGCAGA
CGAAGGAGGGGAACATCTAA

Downstream 100 bases:

>100_bases
TGCAGGCGGAGGTTCACGATCGCAAACGCTACGCGGAGACGGTCAACCCTACCACTACGGATGGGCGCGACATCGACACG
TCGATCGGCACGCTGTCCGG

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MRALTAAEAHHVEMRAARDDTANRTVRRGFFTDTTLCIGCKACEVACKQWNQLPDDGFVFTGMSYDNTVALGASTWRHVA
FIERPVALSTQDGGAFSWLFSSDVCKHCARAGCLESCPTGAIIRTEFDSVYVQPDICNGCGYCVINCPFGVIDRSPDDGR
AWKCTLCYDRMKFDMEPACAKACPTDSIQYGDLDELLNAAQRRVAKLHENGMQEAYLYGADAASQPGTEGLNAFFLLVDR
PEVYNLPPNPVVPTKTVRETWRSFAWTVAGLAAVAVGSVFSAKGVR

Sequences:

>Translated_286_residues
MRALTAAEAHHVEMRAARDDTANRTVRRGFFTDTTLCIGCKACEVACKQWNQLPDDGFVFTGMSYDNTVALGASTWRHVA
FIERPVALSTQDGGAFSWLFSSDVCKHCARAGCLESCPTGAIIRTEFDSVYVQPDICNGCGYCVINCPFGVIDRSPDDGR
AWKCTLCYDRMKFDMEPACAKACPTDSIQYGDLDELLNAAQRRVAKLHENGMQEAYLYGADAASQPGTEGLNAFFLLVDR
PEVYNLPPNPVVPTKTVRETWRSFAWTVAGLAAVAVGSVFSAKGVR
>Mature_286_residues
MRALTAAEAHHVEMRAARDDTANRTVRRGFFTDTTLCIGCKACEVACKQWNQLPDDGFVFTGMSYDNTVALGASTWRHVA
FIERPVALSTQDGGAFSWLFSSDVCKHCARAGCLESCPTGAIIRTEFDSVYVQPDICNGCGYCVINCPFGVIDRSPDDGR
AWKCTLCYDRMKFDMEPACAKACPTDSIQYGDLDELLNAAQRRVAKLHENGMQEAYLYGADAASQPGTEGLNAFFLLVDR
PEVYNLPPNPVVPTKTVRETWRSFAWTVAGLAAVAVGSVFSAKGVR

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=259, Percent_Identity=37.8378378378378, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790326, Length=261, Percent_Identity=38.6973180076628, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1787122, Length=176, Percent_Identity=31.25, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787872, Length=176, Percent_Identity=30.1136363636364, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI2367345, Length=175, Percent_Identity=32, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=27.1739130434783, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI226510944, Length=172, Percent_Identity=30.8139534883721, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI2367245, Length=173, Percent_Identity=31.7919075144509, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788811, Length=171, Percent_Identity=28.0701754385965, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789067, Length=199, Percent_Identity=31.1557788944724, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=174, Percent_Identity=32.7586206896552, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082179, Length=180, Percent_Identity=29.4444444444444, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787478, Length=86, Percent_Identity=31.3953488372093, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 31300; Mature: 31300

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALTAAEAHHVEMRAARDDTANRTVRRGFFTDTTLCIGCKACEVACKQWNQLPDDGFVF
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCEEE
TGMSYDNTVALGASTWRHVAFIERPVALSTQDGGAFSWLFSSDVCKHCARAGCLESCPTG
ECCCCCCEEEECCCHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCC
AIIRTEFDSVYVQPDICNGCGYCVINCPFGVIDRSPDDGRAWKCTLCYDRMKFDMEPACA
CEEEEECCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEHHCCCCCHHHH
KACPTDSIQYGDLDELLNAAQRRVAKLHENGMQEAYLYGADAASQPGTEGLNAFFLLVDR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEEEEC
PEVYNLPPNPVVPTKTVRETWRSFAWTVAGLAAVAVGSVFSAKGVR
CCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRALTAAEAHHVEMRAARDDTANRTVRRGFFTDTTLCIGCKACEVACKQWNQLPDDGFVF
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCEEE
TGMSYDNTVALGASTWRHVAFIERPVALSTQDGGAFSWLFSSDVCKHCARAGCLESCPTG
ECCCCCCEEEECCCHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCC
AIIRTEFDSVYVQPDICNGCGYCVINCPFGVIDRSPDDGRAWKCTLCYDRMKFDMEPACA
CEEEEECCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEHHCCCCCHHHH
KACPTDSIQYGDLDELLNAAQRRVAKLHENGMQEAYLYGADAASQPGTEGLNAFFLLVDR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEEEEC
PEVYNLPPNPVVPTKTVRETWRSFAWTVAGLAAVAVGSVFSAKGVR
CCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]