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The map label for this gene is pinE [H]

Identifier: 116622060

GI number: 116622060

Start: 3727839

End: 3728480

Strand: Direct

Name: pinE [H]

Synonym: Acid_2947

Alternate gene names: 116622060

Gene position: 3727839-3728480 (Clockwise)

Preceding gene: 116622059

Following gene: 116622062

Centisome position: 37.41

GC content: 63.55

Gene sequence:

>642_bases
ATGGCCGCCAAACGGACATCCGAGAAGGCAAAATCGCCGAGGGTTTTTGGCCAGACACGGAAACTGCCGAAAATGTTTCG
CGCCGGCCTCTACGCCCGCGTCTCTACGAACGACCAGCAGACCGTCCCGCTGCAGACCCGCGCCCTGCGGGAATATACCG
TCCGGCGGGGTTGGACGGTCGCTCTGCAGGTGAAAGAGGTCCGTAGCGGTGCATCCGAACGGCAACTGCGGGAGAAGTTG
TTGGCGGCGGCGCGCCGCGGCGAGATCGACGTGGTGCTGGTGTGGCGATTGGATCGTTGGGACAGATCGGTGGCGGACCT
GCTGGCAACTCTGCAGGAACTGGAGCATCTGGGTGTCGGCTTCGTCTCGCTGACAGAGGCTCTGGACCTGACGACGCCCG
CCGGTCGCGCCATGGCTGCACTACTTGCTGTATTCGCGGAGTTCGAAAGAGAAATTCTACGGGAACGCGTGCGCGCCGGC
CTAGCCCACGCCCGACAGAATGGTCAGACCCTGGGCCGACCCGTAACAGCCGGACTCCAGGCGGACAAGGCTCGAAAACT
GTATCTTGCCGGCGTCAGCAAAGCCGAGATCGCCCGTCGCTTGCAGATCGGTCGCACCTCCGTTCGCCGCATTCTGGCCT
GA

Upstream 100 bases:

>100_bases
CTATCGCGAGACGCTGGCGCACGCGCCTTCTGGGTAATATCATGATTTCCAGCATGGCCGAAAAGGATAGTCGTCGGCCA
TGCAGGAGCGACACCATCAA

Downstream 100 bases:

>100_bases
CCTATTTCTTGAAGAAATAGGTCAAGCCTCGCGAACCTCTTCATAGAGAAACTTCCCCCGGATGCTCTCGGCAAAATACC
CGCCTTTAGACTCTGCTGCA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MAAKRTSEKAKSPRVFGQTRKLPKMFRAGLYARVSTNDQQTVPLQTRALREYTVRRGWTVALQVKEVRSGASERQLREKL
LAAARRGEIDVVLVWRLDRWDRSVADLLATLQELEHLGVGFVSLTEALDLTTPAGRAMAALLAVFAEFEREILRERVRAG
LAHARQNGQTLGRPVTAGLQADKARKLYLAGVSKAEIARRLQIGRTSVRRILA

Sequences:

>Translated_213_residues
MAAKRTSEKAKSPRVFGQTRKLPKMFRAGLYARVSTNDQQTVPLQTRALREYTVRRGWTVALQVKEVRSGASERQLREKL
LAAARRGEIDVVLVWRLDRWDRSVADLLATLQELEHLGVGFVSLTEALDLTTPAGRAMAALLAVFAEFEREILRERVRAG
LAHARQNGQTLGRPVTAGLQADKARKLYLAGVSKAEIARRLQIGRTSVRRILA
>Mature_212_residues
AAKRTSEKAKSPRVFGQTRKLPKMFRAGLYARVSTNDQQTVPLQTRALREYTVRRGWTVALQVKEVRSGASERQLREKLL
AAARRGEIDVVLVWRLDRWDRSVADLLATLQELEHLGVGFVSLTEALDLTTPAGRAMAALLAVFAEFEREILRERVRAGL
AHARQNGQTLGRPVTAGLQADKARKLYLAGVSKAEIARRLQIGRTSVRRILA

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=181, Percent_Identity=36.4640883977901, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787638, Length=195, Percent_Identity=32.3076923076923, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1787827, Length=189, Percent_Identity=32.2751322751323, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23823; Mature: 23692

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKRTSEKAKSPRVFGQTRKLPKMFRAGLYARVSTNDQQTVPLQTRALREYTVRRGWTV
CCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEE
ALQVKEVRSGASERQLREKLLAAARRGEIDVVLVWRLDRWDRSVADLLATLQELEHLGVG
EEEHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCH
FVSLTEALDLTTPAGRAMAALLAVFAEFEREILRERVRAGLAHARQNGQTLGRPVTAGLQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
ADKARKLYLAGVSKAEIARRLQIGRTSVRRILA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAKRTSEKAKSPRVFGQTRKLPKMFRAGLYARVSTNDQQTVPLQTRALREYTVRRGWTV
CCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEE
ALQVKEVRSGASERQLREKLLAAARRGEIDVVLVWRLDRWDRSVADLLATLQELEHLGVG
EEEHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCH
FVSLTEALDLTTPAGRAMAALLAVFAEFEREILRERVRAGLAHARQNGQTLGRPVTAGLQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
ADKARKLYLAGVSKAEIARRLQIGRTSVRRILA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]