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The map label for this gene is narK [H]

Identifier: 116622044

GI number: 116622044

Start: 3710733

End: 3712952

Strand: Reverse

Name: narK [H]

Synonym: Acid_2929

Alternate gene names: 116622044

Gene position: 3712952-3710733 (Counterclockwise)

Preceding gene: 116622050

Following gene: 116622043

Centisome position: 37.26

GC content: 59.5

Gene sequence:

>2220_bases
ATGACCAACCGCCGGCACGTCACATTGGGCACGATCTCGTTCATGGTCTGTTTTGCGGCTTGGGGCTTGATCGCCGCTTT
CGCCCCTCGCTTCCGCGAGACCTTTCACCTGACACCCTCACAGACCGCCTTGCTGGTTGCCATCCCGGTGCTCCTCGGTT
CACTGGCCCGCATCCCCATGGGCATTCTGACCGATCGCTTTGGCGGCCGTGCAATCTTCTCAATATTAATGGCCGCCGTG
GCGATTCCCGTGTGGATCGTGCCCGAACAGTCCACCTATTCAGGCCTATTGATAGTTGCGTTCCTCCTGGGCTTGGCCGG
ATCTTCATTCGCCATCGGGGTGGGCTACGTCTCCCGTTGGACCCCGCCGGAACGTCAGGGGAGCGCGCTCGGAGTCTACG
GACTTGGGAACATCGGGCAGTCTGCCGCGGTATTTCTTGGCCCCGTAATCGCGGCGAAGGTTGGAATGGTATCGGTCTAT
CACGGTGTCGCCGCCATGCTGGTAATTTGGGCGATAGCATTCGGTTTGCTGGCGCGCAATGCTCCCGCCCCGGCGACTGC
CCAGCGGAAAGGCTTCGGTGTGATGATCGAGGTGCTTACCCGTGAGAAACTGTCCTGGCTGCTTTCGGGCTTTTACTTCC
TAACGTTTGGCGGCTTCGTCGCCTTTTCGATATATCTGCCGATCTTGCTGAAGGATGAATTCCATTTGACGCCAGCAGAT
GCGGGATTCCGAACTGCCGGCTTCGTGGTTTTGGCGACACTGCTTCGACCCCTGGGCGGATGGCTGTCGGACCGCATCGG
GGGAGCCCGCGTATTGTCCGCTGTCTTCCTCGGCGTGGCGCCCTTTGCGCTGCTTATGTCCTGGTCATCGATGATTCCGT
TTACGGTGGGGGCTCTGGGCTGCGCAGCGCTGCTCGGTTTAGGGAATGGTGCAGTCTTCAAACTCGTGCCGCAGTACTTC
CCGACTCAGACTGGGACGGTCACGGGACTGGTCGGCGCAATGGGAGGGCTCGGCGGCTTCTTTCCGCCCCTCCTGCTGGC
CTTCTTCCGCAGCCAGACCGGGGCAATCTGGCCTGGTTTCGCTTTACTAGCGATTACCTCGTGGCTGTTATGGTGGGCCA
ATCACAAAGTCTTCGTACCTCGGCAACAGGCTCTCGATATCGCCTTGCCGGCCGATCTGCGTCGGACCGCGGATCGCGTG
CAGGCCGGAGCGTGGGCCACTCTGTGGACCGCGTTGCTGGTCGCCGCAATTGTCGTGGGTTCTCGAAATCTGCAGAACTT
CGATCCGGCATTGGTCATTTACACCTTTGCCGTCGTTTTCGCCACATGGGGCGTGGTTTACCATTACAACGTTTGGCTCG
AAAAACCGCCGACGCGAATGTATTGGGATCGGGGCTGGGAGTTGTACCGCCGGAGAGGCGTCATCCGCAGCCTGTTTCGT
GTGGGCACACTTGCCGCAACGCATCTGGCTGGCCAAGGGTTTATTGCCAAGCGTTCCCGCCTGAGGTGGTGGATGCATCA
ATGCCTTTTCTGGGGATGCATGCTGGCCGTCGCCATCACGTTTCCGCTTGTCTTCGGCTGGATCCACTTCCGGACCCTTC
TTGGGGACCAATTGACCTACGTCACGTATCTGTTCGGCTTCCCGATGGGAGCCTTTCGCCTCCACACGATAACGGCGTGG
CTGCTCTTTCATGGTTTGGACATTTCGGCATTGCTGGTATTGGCTGGCATTGCGCTCTCCCTTTGGCGCCGGATGACCGA
CAAGGGAGCCCGCACTTTGCAGCAGTTCGGCATGGACTTCCTGCCGATCATCATGCTTTTCGCGATTTCCGTAACCGGCC
TGGCGCTGACCGTTTCGCAGGAATGGCTCCGTGGTTCTTCGTATAGCTTCCTGGCGATTCTGCATGCGATCACTGTGATA
GCCGCGCTGCTCTTCTTGCCGTTTGGCAAGTTCTTCCACATCTTCCAGCGGCCCGCCCAGCTCGGCGTCAAACTCTACCA
GGAGGCCGGCGTCAAAGACCCCGGCGCTCACTGTGTACGGTGTGGCGAGCGGTTCGCCTCGAAAATGCATATCGACGACC
TCCGCGCGGTGCTTCCGCAGGTTGGGTTCGATTACGCCACACCCGGTCCGGCCGGCCATTGGCAGGCTCTATGTCCGGCC
TGTAAACGAAAATCGCTGTCTACCGCCCAACTCAGAATCAAGGAGGAATCACGTGGCTAA

Upstream 100 bases:

>100_bases
CCCGGAACTCAGAATAGTTTTGTTGCCGTTACTAGGGCAGTGACCTCAGTCACCACGCCAAGAATCGGCAAACGGTAGAC
TTGGGTATCGAGAGAGGGAT

Downstream 100 bases:

>100_bases
AGTGCCGGGTTCACAAGACGAACGGACAAGCCGCTACGGTCCTCATTTGAATTACACACCGCCAGGCGGCTGGAAAGGTG
AGTCGGGAAAGGGCGGAGAG

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MTNRRHVTLGTISFMVCFAAWGLIAAFAPRFRETFHLTPSQTALLVAIPVLLGSLARIPMGILTDRFGGRAIFSILMAAV
AIPVWIVPEQSTYSGLLIVAFLLGLAGSSFAIGVGYVSRWTPPERQGSALGVYGLGNIGQSAAVFLGPVIAAKVGMVSVY
HGVAAMLVIWAIAFGLLARNAPAPATAQRKGFGVMIEVLTREKLSWLLSGFYFLTFGGFVAFSIYLPILLKDEFHLTPAD
AGFRTAGFVVLATLLRPLGGWLSDRIGGARVLSAVFLGVAPFALLMSWSSMIPFTVGALGCAALLGLGNGAVFKLVPQYF
PTQTGTVTGLVGAMGGLGGFFPPLLLAFFRSQTGAIWPGFALLAITSWLLWWANHKVFVPRQQALDIALPADLRRTADRV
QAGAWATLWTALLVAAIVVGSRNLQNFDPALVIYTFAVVFATWGVVYHYNVWLEKPPTRMYWDRGWELYRRRGVIRSLFR
VGTLAATHLAGQGFIAKRSRLRWWMHQCLFWGCMLAVAITFPLVFGWIHFRTLLGDQLTYVTYLFGFPMGAFRLHTITAW
LLFHGLDISALLVLAGIALSLWRRMTDKGARTLQQFGMDFLPIIMLFAISVTGLALTVSQEWLRGSSYSFLAILHAITVI
AALLFLPFGKFFHIFQRPAQLGVKLYQEAGVKDPGAHCVRCGERFASKMHIDDLRAVLPQVGFDYATPGPAGHWQALCPA
CKRKSLSTAQLRIKEESRG

Sequences:

>Translated_739_residues
MTNRRHVTLGTISFMVCFAAWGLIAAFAPRFRETFHLTPSQTALLVAIPVLLGSLARIPMGILTDRFGGRAIFSILMAAV
AIPVWIVPEQSTYSGLLIVAFLLGLAGSSFAIGVGYVSRWTPPERQGSALGVYGLGNIGQSAAVFLGPVIAAKVGMVSVY
HGVAAMLVIWAIAFGLLARNAPAPATAQRKGFGVMIEVLTREKLSWLLSGFYFLTFGGFVAFSIYLPILLKDEFHLTPAD
AGFRTAGFVVLATLLRPLGGWLSDRIGGARVLSAVFLGVAPFALLMSWSSMIPFTVGALGCAALLGLGNGAVFKLVPQYF
PTQTGTVTGLVGAMGGLGGFFPPLLLAFFRSQTGAIWPGFALLAITSWLLWWANHKVFVPRQQALDIALPADLRRTADRV
QAGAWATLWTALLVAAIVVGSRNLQNFDPALVIYTFAVVFATWGVVYHYNVWLEKPPTRMYWDRGWELYRRRGVIRSLFR
VGTLAATHLAGQGFIAKRSRLRWWMHQCLFWGCMLAVAITFPLVFGWIHFRTLLGDQLTYVTYLFGFPMGAFRLHTITAW
LLFHGLDISALLVLAGIALSLWRRMTDKGARTLQQFGMDFLPIIMLFAISVTGLALTVSQEWLRGSSYSFLAILHAITVI
AALLFLPFGKFFHIFQRPAQLGVKLYQEAGVKDPGAHCVRCGERFASKMHIDDLRAVLPQVGFDYATPGPAGHWQALCPA
CKRKSLSTAQLRIKEESRG
>Mature_738_residues
TNRRHVTLGTISFMVCFAAWGLIAAFAPRFRETFHLTPSQTALLVAIPVLLGSLARIPMGILTDRFGGRAIFSILMAAVA
IPVWIVPEQSTYSGLLIVAFLLGLAGSSFAIGVGYVSRWTPPERQGSALGVYGLGNIGQSAAVFLGPVIAAKVGMVSVYH
GVAAMLVIWAIAFGLLARNAPAPATAQRKGFGVMIEVLTREKLSWLLSGFYFLTFGGFVAFSIYLPILLKDEFHLTPADA
GFRTAGFVVLATLLRPLGGWLSDRIGGARVLSAVFLGVAPFALLMSWSSMIPFTVGALGCAALLGLGNGAVFKLVPQYFP
TQTGTVTGLVGAMGGLGGFFPPLLLAFFRSQTGAIWPGFALLAITSWLLWWANHKVFVPRQQALDIALPADLRRTADRVQ
AGAWATLWTALLVAAIVVGSRNLQNFDPALVIYTFAVVFATWGVVYHYNVWLEKPPTRMYWDRGWELYRRRGVIRSLFRV
GTLAATHLAGQGFIAKRSRLRWWMHQCLFWGCMLAVAITFPLVFGWIHFRTLLGDQLTYVTYLFGFPMGAFRLHTITAWL
LFHGLDISALLVLAGIALSLWRRMTDKGARTLQQFGMDFLPIIMLFAISVTGLALTVSQEWLRGSSYSFLAILHAITVIA
ALLFLPFGKFFHIFQRPAQLGVKLYQEAGVKDPGAHCVRCGERFASKMHIDDLRAVLPQVGFDYATPGPAGHWQALCPAC
KRKSLSTAQLRIKEESRG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=397, Percent_Identity=28.2115869017632, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787743, Length=406, Percent_Identity=29.064039408867, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560030, Length=358, Percent_Identity=21.7877094972067, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 80872; Mature: 80740

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRRHVTLGTISFMVCFAAWGLIAAFAPRFRETFHLTPSQTALLVAIPVLLGSLARIPM
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCH
GILTDRFGGRAIFSILMAAVAIPVWIVPEQSTYSGLLIVAFLLGLAGSSFAIGVGYVSRW
HHHHHCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHCC
TPPERQGSALGVYGLGNIGQSAAVFLGPVIAAKVGMVSVYHGVAAMLVIWAIAFGLLARN
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APAPATAQRKGFGVMIEVLTREKLSWLLSGFYFLTFGGFVAFSIYLPILLKDEFHLTPAD
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AGFRTAGFVVLATLLRPLGGWLSDRIGGARVLSAVFLGVAPFALLMSWSSMIPFTVGALG
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
CAALLGLGNGAVFKLVPQYFPTQTGTVTGLVGAMGGLGGFFPPLLLAFFRSQTGAIWPGF
HHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHH
ALLAITSWLLWWANHKVFVPRQQALDIALPADLRRTADRVQAGAWATLWTALLVAAIVVG
HHHHHHHHHHHHHCCEEECCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
SRNLQNFDPALVIYTFAVVFATWGVVYHYNVWLEKPPTRMYWDRGWELYRRRGVIRSLFR
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHH
VGTLAATHLAGQGFIAKRSRLRWWMHQCLFWGCMLAVAITFPLVFGWIHFRTLLGDQLTY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VTYLFGFPMGAFRLHTITAWLLFHGLDISALLVLAGIALSLWRRMTDKGARTLQQFGMDF
HHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LPIIMLFAISVTGLALTVSQEWLRGSSYSFLAILHAITVIAALLFLPFGKFFHIFQRPAQ
HHHHHHHHHHHHHHHEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVKLYQEAGVKDPGAHCVRCGERFASKMHIDDLRAVLPQVGFDYATPGPAGHWQALCPA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
CKRKSLSTAQLRIKEESRG
HHCCCCCHHHEEECCCCCC
>Mature Secondary Structure 
TNRRHVTLGTISFMVCFAAWGLIAAFAPRFRETFHLTPSQTALLVAIPVLLGSLARIPM
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCH
GILTDRFGGRAIFSILMAAVAIPVWIVPEQSTYSGLLIVAFLLGLAGSSFAIGVGYVSRW
HHHHHCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHCC
TPPERQGSALGVYGLGNIGQSAAVFLGPVIAAKVGMVSVYHGVAAMLVIWAIAFGLLARN
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APAPATAQRKGFGVMIEVLTREKLSWLLSGFYFLTFGGFVAFSIYLPILLKDEFHLTPAD
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AGFRTAGFVVLATLLRPLGGWLSDRIGGARVLSAVFLGVAPFALLMSWSSMIPFTVGALG
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
CAALLGLGNGAVFKLVPQYFPTQTGTVTGLVGAMGGLGGFFPPLLLAFFRSQTGAIWPGF
HHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHH
ALLAITSWLLWWANHKVFVPRQQALDIALPADLRRTADRVQAGAWATLWTALLVAAIVVG
HHHHHHHHHHHHHCCEEECCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
SRNLQNFDPALVIYTFAVVFATWGVVYHYNVWLEKPPTRMYWDRGWELYRRRGVIRSLFR
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHH
VGTLAATHLAGQGFIAKRSRLRWWMHQCLFWGCMLAVAITFPLVFGWIHFRTLLGDQLTY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VTYLFGFPMGAFRLHTITAWLLFHGLDISALLVLAGIALSLWRRMTDKGARTLQQFGMDF
HHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LPIIMLFAISVTGLALTVSQEWLRGSSYSFLAILHAITVIAALLFLPFGKFFHIFQRPAQ
HHHHHHHHHHHHHHHEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVKLYQEAGVKDPGAHCVRCGERFASKMHIDDLRAVLPQVGFDYATPGPAGHWQALCPA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
CKRKSLSTAQLRIKEESRG
HHCCCCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]