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The map label for this gene is nasC [H]

Identifier: 116622043

GI number: 116622043

Start: 3708548

End: 3710740

Strand: Reverse

Name: nasC [H]

Synonym: Acid_2928

Alternate gene names: 116622043

Gene position: 3710740-3708548 (Counterclockwise)

Preceding gene: 116622044

Following gene: 116622042

Centisome position: 37.24

GC content: 60.51

Gene sequence:

>2193_bases
GTGGCTAAAGTGCCGGGTTCACAAGACGAACGGACAAGCCGCTACGGTCCTCATTTGAATTACACACCGCCAGGCGGCTG
GAAAGGTGAGTCGGGAAAGGGCGGAGAGAACCTCGTAAAGACCCACTGTTGCTTTTGCGGCCAGCAGTGCGGCATTCAGC
TCAAGGTCCGCGACAACCGCGTGATGGGCTTCGAGCCATGGGAGGAGTTTCCCTTCAACCGCGGAATGCTTTGCCCCAAG
GGCGTTAAGCGTTACATGCAGGGCAGCCACCCCGACCGCCTTCTCGATCCCCTGATGAGAACGCCGGAAGGCTTCCGTCC
CGCGAAGTGGGACGAAGCTCTGGATTTCACGGCGCGGCGTCTCCGTGAGCTGCAAGAGAAATATGGAAAAGATTCCGTGG
CGGTATATGGCGGTGCCTCGCTGACCACCGAGAAATCGTATCTCATGGGCAAGTTTGCGCGTGTGGGGCTGGGAACCCGC
CACATCGACTACAACGGACGGCTCTGCATGGTTTCCGCCGGCACCGCCTACAAGACGGCGCTGGGTGTCGATCGCGCGCC
AAACCCCTGGAACGACATTTCCGAAGCCGAAGTCCTCTTCGTCATCGGCGCCAACATCGGTGAGTGTGCACCCATCACCA
CCGACTACATCTGGCGCGCCCGTGACAAGGGCGCCAAGTTGATTGTTGCCGATCCGCGCTTTACTCCCATTGCGCGCAAT
GCGGACCTGTATCTCCCGGTGCGGCCCGGGACCGATCTCGCGCTGCTAATGGGAATGCTTCACGTAATCATTCGCGACGG
CCTGACGGATCCAGCTTTCATCGAGGCGCACACTACCGGATTCGAGAAGACCGCGGAATCGGTAGAAGCCTGGAATCCAC
GACGCGCGGCCGAGGTGAGTGGCGTCGCGCCGGAGGCCATCGAGAAGGCAGCGCATCTAATTGGCGAGTCGCGGCGGGCG
ATGCTCATGCACGCGCGCGGTCTGGAACACCAGTCCAAGGGCGTCGAGAATTGCGAAGCCGTAGTCGCCATCGCACTGGC
AACTGGCAATATCGGTCGCGAGGGTGCCGGACCAATCATGGTGACCGGTCAGGGCAACGGCCAGGGCGGTCGCGAACACG
GGCAGAAGTGCGACCAATTGCCCGGCCAACGAAGCCTCACGGATCCTGCAGCGCGTGCGCATGTGGCGGGAGTCTGGGGG
ATCTCACCGGACGATTTGCCGCAACCGGGCTACACAGCAGTTGAGATCATGAACGCCATTCACAGCGGCGAAATCAAAGG
GCTGCTCTCCATCTGCTTCAACCCGCTGGTATCCCTGCCAGATGCGAACTTTACGCGCGAAGCCCTGGAAAAGCTCGAGT
TTTTTGCCGTGATCGACTTCTTTCTTTCGGAAACGGCGGCGCACGCGGATGTTGTACTGGCCGGCAGCCTGCAGGAGGAA
GAAGAAGGCGTAACCGCCAACGTGGAAGCGCGGGTCATCCATATCAAGAAGGCCGTCGACCCGCCTGGCAATGCTCGCGC
AGACTCCTCCATCATCTGCGACCTGGCACACCGACTGGGTCGCGGCCAGTTTTTCCCCTTTAAAGAGCCGCGCGAGATCT
TTGAAGAATTACGCCGAGCCTCGCAAGGCGGCCTCGCCGACTACTACGGCATCACCTACGAGAAGATCGACCAACAGATG
GGCGTGTTCTGGCCTTGCCCCACGCTCGATCATCCCGGCACGCCCCGACTGTTTGAAGACGGCCGCTTCTTCCATCCTGA
CGGCAAAGCGCGCTTCATGAGTCTCGTTTGGCGGGATAGCGGCGATCCGGTGGACGCTGAGTTCTCAATCTACCTCACCA
CGGGCCGTGTAGTAAGCCAATATCTGTCAGGTACGCAGACCCGCCGGATCGGAGCGTTGGTCGATCAATATCCCGAACCG
AAGCTGGAACTTCATCCGCGTTTGGCGGAACAACACAAGATCAACAGCGGCGATTGGGTCACGGTGACGTCCCGCCGGAC
CGCGATCACTTTACAGGCGATGGTGGTGCGCACCATCCGGCCCGACACCGTGTTCATTCCGTACCACTGGCCAGGTGGTA
GAAGCGCGAACCGGCTGACCCATCGAACGCTGGACCCACGGAGTAAGATTCCCGAGTACAAAGTGTCGGCGTGCCGCATC
GAGAAGGCTGTCGCTCCAGGAGGTCAGTCGTAA

Upstream 100 bases:

>100_bases
TACGCCACACCCGGTCCGGCCGGCCATTGGCAGGCTCTATGTCCGGCCTGTAAACGAAAATCGCTGTCTACCGCCCAACT
CAGAATCAAGGAGGAATCAC

Downstream 100 bases:

>100_bases
TGTTCGGGATGGAGTTTTACATCGACCCCTCACGCTGCATCGGATGTCAGAGCTGTCTGAAGGCCTGCGAGGAATGCGAT
ACGCACCGCGGCATCTCGAT

Product: assimilatory nitrate reductase (NADH) subunit alpha apoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 729

Protein sequence:

>730_residues
MAKVPGSQDERTSRYGPHLNYTPPGGWKGESGKGGENLVKTHCCFCGQQCGIQLKVRDNRVMGFEPWEEFPFNRGMLCPK
GVKRYMQGSHPDRLLDPLMRTPEGFRPAKWDEALDFTARRLRELQEKYGKDSVAVYGGASLTTEKSYLMGKFARVGLGTR
HIDYNGRLCMVSAGTAYKTALGVDRAPNPWNDISEAEVLFVIGANIGECAPITTDYIWRARDKGAKLIVADPRFTPIARN
ADLYLPVRPGTDLALLMGMLHVIIRDGLTDPAFIEAHTTGFEKTAESVEAWNPRRAAEVSGVAPEAIEKAAHLIGESRRA
MLMHARGLEHQSKGVENCEAVVAIALATGNIGREGAGPIMVTGQGNGQGGREHGQKCDQLPGQRSLTDPAARAHVAGVWG
ISPDDLPQPGYTAVEIMNAIHSGEIKGLLSICFNPLVSLPDANFTREALEKLEFFAVIDFFLSETAAHADVVLAGSLQEE
EEGVTANVEARVIHIKKAVDPPGNARADSSIICDLAHRLGRGQFFPFKEPREIFEELRRASQGGLADYYGITYEKIDQQM
GVFWPCPTLDHPGTPRLFEDGRFFHPDGKARFMSLVWRDSGDPVDAEFSIYLTTGRVVSQYLSGTQTRRIGALVDQYPEP
KLELHPRLAEQHKINSGDWVTVTSRRTAITLQAMVVRTIRPDTVFIPYHWPGGRSANRLTHRTLDPRSKIPEYKVSACRI
EKAVAPGGQS

Sequences:

>Translated_730_residues
MAKVPGSQDERTSRYGPHLNYTPPGGWKGESGKGGENLVKTHCCFCGQQCGIQLKVRDNRVMGFEPWEEFPFNRGMLCPK
GVKRYMQGSHPDRLLDPLMRTPEGFRPAKWDEALDFTARRLRELQEKYGKDSVAVYGGASLTTEKSYLMGKFARVGLGTR
HIDYNGRLCMVSAGTAYKTALGVDRAPNPWNDISEAEVLFVIGANIGECAPITTDYIWRARDKGAKLIVADPRFTPIARN
ADLYLPVRPGTDLALLMGMLHVIIRDGLTDPAFIEAHTTGFEKTAESVEAWNPRRAAEVSGVAPEAIEKAAHLIGESRRA
MLMHARGLEHQSKGVENCEAVVAIALATGNIGREGAGPIMVTGQGNGQGGREHGQKCDQLPGQRSLTDPAARAHVAGVWG
ISPDDLPQPGYTAVEIMNAIHSGEIKGLLSICFNPLVSLPDANFTREALEKLEFFAVIDFFLSETAAHADVVLAGSLQEE
EEGVTANVEARVIHIKKAVDPPGNARADSSIICDLAHRLGRGQFFPFKEPREIFEELRRASQGGLADYYGITYEKIDQQM
GVFWPCPTLDHPGTPRLFEDGRFFHPDGKARFMSLVWRDSGDPVDAEFSIYLTTGRVVSQYLSGTQTRRIGALVDQYPEP
KLELHPRLAEQHKINSGDWVTVTSRRTAITLQAMVVRTIRPDTVFIPYHWPGGRSANRLTHRTLDPRSKIPEYKVSACRI
EKAVAPGGQS
>Mature_729_residues
AKVPGSQDERTSRYGPHLNYTPPGGWKGESGKGGENLVKTHCCFCGQQCGIQLKVRDNRVMGFEPWEEFPFNRGMLCPKG
VKRYMQGSHPDRLLDPLMRTPEGFRPAKWDEALDFTARRLRELQEKYGKDSVAVYGGASLTTEKSYLMGKFARVGLGTRH
IDYNGRLCMVSAGTAYKTALGVDRAPNPWNDISEAEVLFVIGANIGECAPITTDYIWRARDKGAKLIVADPRFTPIARNA
DLYLPVRPGTDLALLMGMLHVIIRDGLTDPAFIEAHTTGFEKTAESVEAWNPRRAAEVSGVAPEAIEKAAHLIGESRRAM
LMHARGLEHQSKGVENCEAVVAIALATGNIGREGAGPIMVTGQGNGQGGREHGQKCDQLPGQRSLTDPAARAHVAGVWGI
SPDDLPQPGYTAVEIMNAIHSGEIKGLLSICFNPLVSLPDANFTREALEKLEFFAVIDFFLSETAAHADVVLAGSLQEEE
EGVTANVEARVIHIKKAVDPPGNARADSSIICDLAHRLGRGQFFPFKEPREIFEELRRASQGGLADYYGITYEKIDQQMG
VFWPCPTLDHPGTPRLFEDGRFFHPDGKARFMSLVWRDSGDPVDAEFSIYLTTGRVVSQYLSGTQTRRIGALVDQYPEPK
LELHPRLAEQHKINSGDWVTVTSRRTAITLQAMVVRTIRPDTVFIPYHWPGGRSANRLTHRTLDPRSKIPEYKVSACRIE
KAVAPGGQS

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=697, Percent_Identity=32.5681492109039, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI1788534, Length=793, Percent_Identity=24.968474148802, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI3868719, Length=679, Percent_Identity=24.1531664212077, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI3868720, Length=616, Percent_Identity=25.3246753246753, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1787870, Length=730, Percent_Identity=24.7945205479452, Blast_Score=146, Evalue=6e-36,
Organism=Escherichia coli, GI171474008, Length=556, Percent_Identity=25.5395683453237, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87081797, Length=749, Percent_Identity=23.7650200267023, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1787778, Length=686, Percent_Identity=22.7405247813411, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI145693196, Length=662, Percent_Identity=23.5649546827795, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081994, Length=531, Percent_Identity=25.0470809792844, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 80434; Mature: 80303

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVPGSQDERTSRYGPHLNYTPPGGWKGESGKGGENLVKTHCCFCGQQCGIQLKVRDNR
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCE
VMGFEPWEEFPFNRGMLCPKGVKRYMQGSHPDRLLDPLMRTPEGFRPAKWDEALDFTARR
EECCCCHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRELQEKYGKDSVAVYGGASLTTEKSYLMGKFARVGLGTRHIDYNGRLCMVSAGTAYKTA
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECCCHHHHH
LGVDRAPNPWNDISEAEVLFVIGANIGECAPITTDYIWRARDKGAKLIVADPRFTPIARN
HCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHEEECCCCCEEEEECCCCCCEECC
ADLYLPVRPGTDLALLMGMLHVIIRDGLTDPAFIEAHTTGFEKTAESVEAWNPRRAAEVS
CCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHC
GVAPEAIEKAAHLIGESRRAMLMHARGLEHQSKGVENCEAVVAIALATGNIGREGAGPIM
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCHHHHHEEEEEEEECCCCCCCCCCCEE
VTGQGNGQGGREHGQKCDQLPGQRSLTDPAARAHVAGVWGISPDDLPQPGYTAVEIMNAI
EEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHH
HSGEIKGLLSICFNPLVSLPDANFTREALEKLEFFAVIDFFLSETAAHADVVLAGSLQEE
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
EEGVTANVEARVIHIKKAVDPPGNARADSSIICDLAHRLGRGQFFPFKEPREIFEELRRA
HCCCEECCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
SQGGLADYYGITYEKIDQQMGVFWPCPTLDHPGTPRLFEDGRFFHPDGKARFMSLVWRDS
HCCCCHHHHCCHHHHHHHHCCCEECCCCCCCCCCCCEECCCCEECCCCHHEEEEEEECCC
GDPVDAEFSIYLTTGRVVSQYLSGTQTRRIGALVDQYPEPKLELHPRLAEQHKINSGDWV
CCCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCEE
TVTSRRTAITLQAMVVRTIRPDTVFIPYHWPGGRSANRLTHRTLDPRSKIPEYKVSACRI
EEECCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHCCCCHHHCCCCCHHHHHHH
EKAVAPGGQS
HHHCCCCCCC
>Mature Secondary Structure 
AKVPGSQDERTSRYGPHLNYTPPGGWKGESGKGGENLVKTHCCFCGQQCGIQLKVRDNR
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCE
VMGFEPWEEFPFNRGMLCPKGVKRYMQGSHPDRLLDPLMRTPEGFRPAKWDEALDFTARR
EECCCCHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRELQEKYGKDSVAVYGGASLTTEKSYLMGKFARVGLGTRHIDYNGRLCMVSAGTAYKTA
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECCCHHHHH
LGVDRAPNPWNDISEAEVLFVIGANIGECAPITTDYIWRARDKGAKLIVADPRFTPIARN
HCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHEEECCCCCEEEEECCCCCCEECC
ADLYLPVRPGTDLALLMGMLHVIIRDGLTDPAFIEAHTTGFEKTAESVEAWNPRRAAEVS
CCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHC
GVAPEAIEKAAHLIGESRRAMLMHARGLEHQSKGVENCEAVVAIALATGNIGREGAGPIM
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCHHHHHEEEEEEEECCCCCCCCCCCEE
VTGQGNGQGGREHGQKCDQLPGQRSLTDPAARAHVAGVWGISPDDLPQPGYTAVEIMNAI
EEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHH
HSGEIKGLLSICFNPLVSLPDANFTREALEKLEFFAVIDFFLSETAAHADVVLAGSLQEE
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
EEGVTANVEARVIHIKKAVDPPGNARADSSIICDLAHRLGRGQFFPFKEPREIFEELRRA
HCCCEECCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
SQGGLADYYGITYEKIDQQMGVFWPCPTLDHPGTPRLFEDGRFFHPDGKARFMSLVWRDS
HCCCCHHHHCCHHHHHHHHCCCEECCCCCCCCCCCCEECCCCEECCCCHHEEEEEEECCC
GDPVDAEFSIYLTTGRVVSQYLSGTQTRRIGALVDQYPEPKLELHPRLAEQHKINSGDWV
CCCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCEE
TVTSRRTAITLQAMVVRTIRPDTVFIPYHWPGGRSANRLTHRTLDPRSKIPEYKVSACRI
EEECCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHCCCCHHHCCCCCHHHHHHH
EKAVAPGGQS
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]