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The map label for this gene is fdoH [H]

Identifier: 116622042

GI number: 116622042

Start: 3707949

End: 3708548

Strand: Reverse

Name: fdoH [H]

Synonym: Acid_2927

Alternate gene names: 116622042

Gene position: 3708548-3707949 (Counterclockwise)

Preceding gene: 116622043

Following gene: 116622041

Centisome position: 37.21

GC content: 56.83

Gene sequence:

>600_bases
ATGTTCGGGATGGAGTTTTACATCGACCCCTCACGCTGCATCGGATGTCAGAGCTGTCTGAAGGCCTGCGAGGAATGCGA
TACGCACCGCGGCATCTCGATGATCAACTTCGACTTCGTGGACCGTAAGGAGTCCATCGCCACCGCGGCCTACGTATGCT
GGCACTGTGACGAGCCAACATGCGCTCAGGTGTGCCCGGCCGATGCCATCAAGAAGTATGAAGACGGCATTGTCGGCTCC
TCGTTGAAACCGCGCTGCATCGGCTGTTCGAACTGCGTACTGGCGTGCCCCTTCGGAATCCCGAAGCTAATACCGGAATA
CGAGCAGATGATGAAGTGCGACATGTGCTACGACCGCACGTCCGTAGGAAAGAAACCGATGTGCGCGACGGTATGCCCCA
GCCAGGCGTTGACCTATGTGACACCGGAAACGATCGCGGCCACACGGCGCGAAAAGCCCGTGAATACCTTCTGGTTCGGA
AACCAGAAAATCACAACCAAGATCTACATGATGGCTCCGGCCGATGCGGCTGACATTGCAATCGACGTCGCCGATTACAC
GTGGGAGGGAGAGAATGAGCTGGTTTCGCAAGCCTTCTAG

Upstream 100 bases:

>100_bases
GGCTGACCCATCGAACGCTGGACCCACGGAGTAAGATTCCCGAGTACAAAGTGTCGGCGTGCCGCATCGAGAAGGCTGTC
GCTCCAGGAGGTCAGTCGTA

Downstream 100 bases:

>100_bases
TACACCGCTCTGGCGGGATGAATTCTCGGTGCATACGGCCGACGAACGGTACGTGAACCGCCGCCAGTTCACCAAATTTC
TGACGTTGACGAGCCTCGGA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH2 [C]

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MFGMEFYIDPSRCIGCQSCLKACEECDTHRGISMINFDFVDRKESIATAAYVCWHCDEPTCAQVCPADAIKKYEDGIVGS
SLKPRCIGCSNCVLACPFGIPKLIPEYEQMMKCDMCYDRTSVGKKPMCATVCPSQALTYVTPETIAATRREKPVNTFWFG
NQKITTKIYMMAPADAADIAIDVADYTWEGENELVSQAF

Sequences:

>Translated_199_residues
MFGMEFYIDPSRCIGCQSCLKACEECDTHRGISMINFDFVDRKESIATAAYVCWHCDEPTCAQVCPADAIKKYEDGIVGS
SLKPRCIGCSNCVLACPFGIPKLIPEYEQMMKCDMCYDRTSVGKKPMCATVCPSQALTYVTPETIAATRREKPVNTFWFG
NQKITTKIYMMAPADAADIAIDVADYTWEGENELVSQAF
>Mature_199_residues
MFGMEFYIDPSRCIGCQSCLKACEECDTHRGISMINFDFVDRKESIATAAYVCWHCDEPTCAQVCPADAIKKYEDGIVGS
SLKPRCIGCSNCVLACPFGIPKLIPEYEQMMKCDMCYDRTSVGKKPMCATVCPSQALTYVTPETIAATRREKPVNTFWFG
NQKITTKIYMMAPADAADIAIDVADYTWEGENELVSQAF

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=204, Percent_Identity=30.3921568627451, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787872, Length=147, Percent_Identity=38.7755102040816, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1787122, Length=147, Percent_Identity=38.7755102040816, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787749, Length=167, Percent_Identity=31.7365269461078, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI2367245, Length=155, Percent_Identity=33.5483870967742, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082179, Length=159, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI226510944, Length=191, Percent_Identity=29.3193717277487, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082114, Length=192, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082180, Length=136, Percent_Identity=32.3529411764706, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788811, Length=159, Percent_Identity=29.559748427673, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI2367345, Length=164, Percent_Identity=32.3170731707317, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789370, Length=200, Percent_Identity=26, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789067, Length=174, Percent_Identity=30.4597701149425, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789079, Length=187, Percent_Identity=29.9465240641711, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: NA

Molecular weight: Translated: 22152; Mature: 22152

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
13.1 %Cys+Met (Translated Protein)
8.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
13.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGMEFYIDPSRCIGCQSCLKACEECDTHRGISMINFDFVDRKESIATAAYVCWHCDEPT
CCCCEEEECHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHEEEEEEECCCCC
CAQVCPADAIKKYEDGIVGSSLKPRCIGCSNCVLACPFGIPKLIPEYEQMMKCDMCYDRT
HHHHCCHHHHHHHHCCCCCCCCCCCEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCHH
SVGKKPMCATVCPSQALTYVTPETIAATRREKPVNTFWFGNQKITTKIYMMAPADAADIA
CCCCCCCEEEECCCCCEEEECCHHHHHHHCCCCCCEEEECCEEEEEEEEEECCCCCCCEE
IDVADYTWEGENELVSQAF
EEEECCEECCCHHHHHHCC
>Mature Secondary Structure
MFGMEFYIDPSRCIGCQSCLKACEECDTHRGISMINFDFVDRKESIATAAYVCWHCDEPT
CCCCEEEECHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHEEEEEEECCCCC
CAQVCPADAIKKYEDGIVGSSLKPRCIGCSNCVLACPFGIPKLIPEYEQMMKCDMCYDRT
HHHHCCHHHHHHHHCCCCCCCCCCCEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCHH
SVGKKPMCATVCPSQALTYVTPETIAATRREKPVNTFWFGNQKITTKIYMMAPADAADIA
CCCCCCCEEEECCCCCEEEECCHHHHHHHCCCCCCEEEECCEEEEEEEEEECCCCCCCEE
IDVADYTWEGENELVSQAF
EEEECCEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD [C]

Specific reaction: formate + NAD = CO2 + NADH2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]