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The map label for this gene is nei [C]

Identifier: 116621959

GI number: 116621959

Start: 3601841

End: 3602593

Strand: Reverse

Name: nei [C]

Synonym: Acid_2844

Alternate gene names: 116621959

Gene position: 3602593-3601841 (Counterclockwise)

Preceding gene: 116621960

Following gene: 116621958

Centisome position: 36.15

GC content: 64.28

Gene sequence:

>753_bases
ATGCCGGAAGGGGATACGATTTTTCGGACGGCGCGGACGCTGCAAGCGGCGATCGGCGGGCGTGAGGTTACGCGGTTCGA
ATCTGTACTGCCGAAGCTGGAGCGGGTGGACTACGATACTCCGCTAGCTGGCCGCACAGTGGAGAAGGTGGAGGCGAACG
GCAAGTGGTTGCTGATCTACTTTTCGGGTGACCTGATTCTGCTCACGCACATGCTGATGAGCGGGAGCTGGCACATATAC
CGTCCGGGGGAGCGGTGGCAGCGGCCTCGGAACGAGATGCGGATCGTGATCGAAACCGACGCAATGGTAGCGGTGGCGTT
CCGGGTTCCGGTGGCGGAGTTCCATACGGCGCACAGCCTGGCGCGCCGCGAGGGGCTTAATCAGCTCGGCCCATCAGCGC
TGGCGGAGAATTTCGATGCGGAGACGGCGGTCGCGAACCTAGCGTCGCGGCCCGATCTCGAATTGGGGCTGGCTCTGCTC
GATCAGCGATTGATCGCGGGCTTGGGGAATCTCTTCAAGAGTGAAGTGGCGTTCGCGTGCGGGCTCAGTCCATTCCGCAC
CGTCGCGAGCTTGTCGCGGATCCAGTTGGAGGAACTGGTCACGACATCGCGGAGGCTGCTGCAAGCCAGGCCCGTGCCAA
ACGTCTACGGCCGCGCCGGTGAGCCGTGCCGCCGGTGCGGGACGTCGATCCGCAGCGCCAAGCATCGCGACGATGGCCGC
GTCAGTTTCTGGTGCCCGGTGTGCCAGACGTGA

Upstream 100 bases:

>100_bases
GGTTTCGTGAGCTCGGCACCGGGGTTCCAGATGCGGCGGGTGGACGCGGGGTGACTGTGGGCGAACGCTCCCTTGCGGTC
GCGCTCGTTGGGGAAGTGAG

Downstream 100 bases:

>100_bases
CAGCAGGAATTGTGTATGATCGAACAATGTCAGTCGAAGCTAAGCAGTTGGATTCGGGCATCGGAGTGGTGACGATCGCG
GGCCGGCTGGCCCTGGGCGG

Product: endonuclease VIII/DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase SCO5760; Putative AP lyase SCO5760 [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPEGDTIFRTARTLQAAIGGREVTRFESVLPKLERVDYDTPLAGRTVEKVEANGKWLLIYFSGDLILLTHMLMSGSWHIY
RPGERWQRPRNEMRIVIETDAMVAVAFRVPVAEFHTAHSLARREGLNQLGPSALAENFDAETAVANLASRPDLELGLALL
DQRLIAGLGNLFKSEVAFACGLSPFRTVASLSRIQLEELVTTSRRLLQARPVPNVYGRAGEPCRRCGTSIRSAKHRDDGR
VSFWCPVCQT

Sequences:

>Translated_250_residues
MPEGDTIFRTARTLQAAIGGREVTRFESVLPKLERVDYDTPLAGRTVEKVEANGKWLLIYFSGDLILLTHMLMSGSWHIY
RPGERWQRPRNEMRIVIETDAMVAVAFRVPVAEFHTAHSLARREGLNQLGPSALAENFDAETAVANLASRPDLELGLALL
DQRLIAGLGNLFKSEVAFACGLSPFRTVASLSRIQLEELVTTSRRLLQARPVPNVYGRAGEPCRRCGTSIRSAKHRDDGR
VSFWCPVCQT
>Mature_249_residues
PEGDTIFRTARTLQAAIGGREVTRFESVLPKLERVDYDTPLAGRTVEKVEANGKWLLIYFSGDLILLTHMLMSGSWHIYR
PGERWQRPRNEMRIVIETDAMVAVAFRVPVAEFHTAHSLARREGLNQLGPSALAENFDAETAVANLASRPDLELGLALLD
QRLIAGLGNLFKSEVAFACGLSPFRTVASLSRIQLEELVTTSRRLLQARPVPNVYGRAGEPCRRCGTSIRSAKHRDDGRV
SFWCPVCQT

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786932, Length=273, Percent_Identity=31.5018315018315, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790066, Length=230, Percent_Identity=29.5652173913043, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27860; Mature: 27729

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS01242 ZF_FPG_1 ; PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDTIFRTARTLQAAIGGREVTRFESVLPKLERVDYDTPLAGRTVEKVEANGKWLLIY
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEE
FSGDLILLTHMLMSGSWHIYRPGERWQRPRNEMRIVIETDAMVAVAFRVPVAEFHTAHSL
ECCCHHHHHHHHHCCCCEEECCCHHHHCCCCCEEEEEECCCEEEHHHHCCHHHHHHHHHH
ARREGLNQLGPSALAENFDAETAVANLASRPDLELGLALLDQRLIAGLGNLFKSEVAFAC
HHHHCHHHCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSPFRTVASLSRIQLEELVTTSRRLLQARPVPNVYGRAGEPCRRCGTSIRSAKHRDDGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
VSFWCPVCQT
EEEEECCCCC
>Mature Secondary Structure 
PEGDTIFRTARTLQAAIGGREVTRFESVLPKLERVDYDTPLAGRTVEKVEANGKWLLIY
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEE
FSGDLILLTHMLMSGSWHIYRPGERWQRPRNEMRIVIETDAMVAVAFRVPVAEFHTAHSL
ECCCHHHHHHHHHCCCCEEECCCHHHHCCCCCEEEEEECCCEEEHHHHCCHHHHHHHHHH
ARREGLNQLGPSALAENFDAETAVANLASRPDLELGLALLDQRLIAGLGNLFKSEVAFAC
HHHHCHHHCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSPFRTVASLSRIQLEELVTTSRRLLQARPVPNVYGRAGEPCRRCGTSIRSAKHRDDGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
VSFWCPVCQT
EEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]