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The map label for this gene is ddl

Identifier: 116621914

GI number: 116621914

Start: 3546881

End: 3547858

Strand: Reverse

Name: ddl

Synonym: Acid_2799

Alternate gene names: 116621914

Gene position: 3547858-3546881 (Counterclockwise)

Preceding gene: 116621915

Following gene: 116621913

Centisome position: 35.6

GC content: 60.53

Gene sequence:

>978_bases
ATGAAATTACGAGTAGCCGTAATATACGGCGGCCGCACCGGCGAACATGAAGTGTCCGTGCGTTCCGCCAAGTCAATCCT
CGCCGGACTCGACTGCGAGAAGTACGAAACGATCGAATACTTTATCGACCAGAACGGCAAGTGGAGCCCTGGCCCCATCC
TGCCCGAACCCGGCGCTCACGCCAACATCGATGTCGTCTTCCCCGTCCTCCATGGCACTTTCGGCGAGGATGGCACCGTG
CAGGGACTGCTCGAGCTCGCCGACCTGCCCTACGTCGGCGCCGGAGTCCTCGGCTCCGCCGTCTCAATGGATAAGGAGAT
GATGAAGCGTGTCTGCAAGGAACGCATGTTGCCAATCGTCGATTATGTGACCGTTACACGTCAGACACCCAGCATCGTTG
ATGCCTGCCGCCGCCTCCCGTTCCCCATGTTCGTTAAGCCCGCTAATCTCGGCTCCTCGGTCGGCATTTCCAAGGCCCAC
GACGAACAGGAGCTCGAGGCTGCCTTCTCCCTCGCCAAACAGTACGACCGCAAGATCATCGTTGAGCGCGGCATCGAGGG
CCGCGAGCTCGAATGCTCCGTCCTCGGAAATGAAGCCCCCATGGCTTCCCTGCCCTGCGAAATCCTCCCCTCCCGTGAAT
TCTACGATTACGAGGATAAGTACCTCCTCGATATGGCCCGCACCCAGGTCCCCGCCGACCTCCCCGGCGAGCGCACCGAC
GAAATCCGCCGCCTCGCCGTCGAATGCTACCGGGCCGTGGAATGCGAGGGCATGGCCCGCGTCGATTTCTTCCTGGAGCA
CGGGACCAATCGCCTGTACATCAACGAGATCAACACCATCCCTGGCTTCACTTCCATCAGCATGTACCCCAAAATGTGGG
AGCACAGCGGCATCCCCTTCTGCACCCTGCTCGATCAGCTGATCGAGTTCGCCCTGGACCGCCACCGCCAAAAACAGGTG
ACCCGCTTTGCCCGGTAA

Upstream 100 bases:

>100_bases
CGATGGAGGTGGTCGAGGTGAATCCCGTTATCGACGAGAAGAACCGCACTGCCGATTTCGCCGTCGAGCTCATCATGTCC
GGACTAGGAAAACGTATCTT

Downstream 100 bases:

>100_bases
GGCCGGCCTCGTTGCCGGTCTTCTTCTCCTGACATCACCGCTCGCGGCGCAGCCGAAGCCCGCGCACTACACCTGCCGCC
GGGCGTCGTCGCCCATCCGC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKLRVAVIYGGRTGEHEVSVRSAKSILAGLDCEKYETIEYFIDQNGKWSPGPILPEPGAHANIDVVFPVLHGTFGEDGTV
QGLLELADLPYVGAGVLGSAVSMDKEMMKRVCKERMLPIVDYVTVTRQTPSIVDACRRLPFPMFVKPANLGSSVGISKAH
DEQELEAAFSLAKQYDRKIIVERGIEGRELECSVLGNEAPMASLPCEILPSREFYDYEDKYLLDMARTQVPADLPGERTD
EIRRLAVECYRAVECEGMARVDFFLEHGTNRLYINEINTIPGFTSISMYPKMWEHSGIPFCTLLDQLIEFALDRHRQKQV
TRFAR

Sequences:

>Translated_325_residues
MKLRVAVIYGGRTGEHEVSVRSAKSILAGLDCEKYETIEYFIDQNGKWSPGPILPEPGAHANIDVVFPVLHGTFGEDGTV
QGLLELADLPYVGAGVLGSAVSMDKEMMKRVCKERMLPIVDYVTVTRQTPSIVDACRRLPFPMFVKPANLGSSVGISKAH
DEQELEAAFSLAKQYDRKIIVERGIEGRELECSVLGNEAPMASLPCEILPSREFYDYEDKYLLDMARTQVPADLPGERTD
EIRRLAVECYRAVECEGMARVDFFLEHGTNRLYINEINTIPGFTSISMYPKMWEHSGIPFCTLLDQLIEFALDRHRQKQV
TRFAR
>Mature_325_residues
MKLRVAVIYGGRTGEHEVSVRSAKSILAGLDCEKYETIEYFIDQNGKWSPGPILPEPGAHANIDVVFPVLHGTFGEDGTV
QGLLELADLPYVGAGVLGSAVSMDKEMMKRVCKERMLPIVDYVTVTRQTPSIVDACRRLPFPMFVKPANLGSSVGISKAH
DEQELEAAFSLAKQYDRKIIVERGIEGRELECSVLGNEAPMASLPCEILPSREFYDYEDKYLLDMARTQVPADLPGERTD
EIRRLAVECYRAVECEGMARVDFFLEHGTNRLYINEINTIPGFTSISMYPKMWEHSGIPFCTLLDQLIEFALDRHRQKQV
TRFAR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=43.0594900849858, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1786280, Length=318, Percent_Identity=35.5345911949685, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_SOLUE (Q023Q5)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_824070.1
- ProteinModelPortal:   Q023Q5
- SMR:   Q023Q5
- STRING:   Q023Q5
- GeneID:   4429055
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_2799
- NMPDR:   fig|234267.9.peg.2675
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- PhylomeDB:   Q023Q5
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 36542; Mature: 36542

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRVAVIYGGRTGEHEVSVRSAKSILAGLDCEKYETIEYFIDQNGKWSPGPILPEPGAH
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCCCCCCCCCCCCC
ANIDVVFPVLHGTFGEDGTVQGLLELADLPYVGAGVLGSAVSMDKEMMKRVCKERMLPIV
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
DYVTVTRQTPSIVDACRRLPFPMFVKPANLGSSVGISKAHDEQELEAAFSLAKQYDRKII
HHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VERGIEGRELECSVLGNEAPMASLPCEILPSREFYDYEDKYLLDMARTQVPADLPGERTD
HHCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
EIRRLAVECYRAVECEGMARVDFFLEHGTNRLYINEINTIPGFTSISMYPKMWEHSGIPF
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCHHHHHCCCCCH
CTLLDQLIEFALDRHRQKQVTRFAR
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLRVAVIYGGRTGEHEVSVRSAKSILAGLDCEKYETIEYFIDQNGKWSPGPILPEPGAH
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCCCCCCCCCCCCC
ANIDVVFPVLHGTFGEDGTVQGLLELADLPYVGAGVLGSAVSMDKEMMKRVCKERMLPIV
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
DYVTVTRQTPSIVDACRRLPFPMFVKPANLGSSVGISKAHDEQELEAAFSLAKQYDRKII
HHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VERGIEGRELECSVLGNEAPMASLPCEILPSREFYDYEDKYLLDMARTQVPADLPGERTD
HHCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
EIRRLAVECYRAVECEGMARVDFFLEHGTNRLYINEINTIPGFTSISMYPKMWEHSGIPF
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCHHHHHCCCCCH
CTLLDQLIEFALDRHRQKQVTRFAR
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA