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The map label for this gene is capD [H]

Identifier: 116517199

GI number: 116517199

Start: 99217

End: 101067

Strand: Direct

Name: capD [H]

Synonym: SPD_0099

Alternate gene names: 116517199

Gene position: 99217-101067 (Clockwise)

Preceding gene: 116516685

Following gene: 116516732

Centisome position: 4.85

GC content: 42.19

Gene sequence:

>1851_bases
ATGAATAAAAAACTAACAGATTATGTGATTGATCTGGTGGAAATTTTAAATAAACAACAAAAGCAGGTTTTCTGGGGAAT
ATTTGATATTTTCAGTATGGTGGTTTCCATCATTGTATCTTATATCTTATTCTACGGCTTGATTAATCCAGCACCTGTTG
ACTACATTATCTATACGAGTTTGGCCTTCCTGTTCTATCAATTGATGATTGGTTTTTGGGGGTTGAACGCTAGTATTAGT
CGTTACAGCAAGATTACGGATTTCATGAAAATCTTTTTTGGTGTGACTGCCAGCAGTGTCTTGTCATATAGTATCTGTTA
TGCCTTCTTGCCACTCTTCTCCATCCGTTTCATCATTCTCTTTATCTTGTTGAGTACCTTCTTGATTTTATTGCCACGGA
TTACTTGGCAGTTAATCTACTCCAGACGCAAAAAAGGTAGTGGTGATGGAGAACACCGTCGGACCTTCTTGATTGGTGCC
GGTGATGGTGGGGCTCTTTTTATGGATAGTTACCAACATCCAACCAGTGAATTAGAACTGGTCGGTATTTTGGATAAGGA
TTCTAAGAAAAAGGGTCAAAAACTTGGTGGTATTCCTGTTTTGGGCTCTTATGACAATCTGCCTGAATTAGCCAAACGCC
ATCAAATCGAGCGTGTCATCGTTGCGATTCCGTCGCTGGATCCGTCAGAATATGAGCGTATCTTGCAGATGTGTAATAAG
CTGGGTGTCAAATGTTACAAGATGCCTAAGGTTGAAACTGTTGTTCAGGGCCTTCACCAAGCAGGTACTGGCTTCCAAAA
AATTGATATTACGGACCTTTTGGGTCGTCAGGAAATCCGTCTTGACGAATCGCGTCTGGGTGCAGAACTGACAGGTAAGA
CCATCTTAGTCACAGGAGCTGGAGGTTCAATCGGTTCTGAAATCTGTCGTCAAGTTAGTCGCTTCAATCCTGAACGCATT
GTCTTGCTCGGTCATGGGGAAAACTCAATCTACCTTGTTTATCATGAATTGATTCGTAAGTTCCAAGGGATTGATTATGT
ACCTGTGATTGCGGACATTCAAGACTATGATCGTTTGTTGCAAGTCTTTGAGCAGTACAAACCTGCTATTGTTTATCATG
CGGCAGCCCACAAGCATGTTCCTATGATGGAGCGCAATCCAAAAGAAGCCTTCAAAAACAATATCCGTGGAACTTACAAT
GTTGCTAAGGCTGTTGATGAAGCTAAAGTGTCTAAGATGGTTATGATTTCGACAGATAAGGCAGTTAATCCACCAAATGT
TATGGGAGCAACCAAGCGCGTGGCGGAGTTGATTGTCACTGGCTTTAACCAACGTAGCCAATCAACCTACTGTGCAGTTC
GTTTTGGGAATGTTCTTGGTAGCCGTGGTAGTGTCATTCCAGTCTTTGAACGTCAGATTGCTGAAGGTGGGCCTGTAACG
GTGACAGACTTCCGCATGACCCGTTACTTTATGACCATTCCAGAAGCTAGCCGTCTGGTTATCCATGCTGGTGCTTATGC
CAAAGATGGGGAAGTCTTTATCCTTGATATGGGCAAACCAGTCAAGATTTATGACTTGGCCAAGAAGATGGTGCTTCTAA
GTGGCCACACTGAAAGTGAAATTCCAATCGTTGAAGTTGGAATCCGCCCAGGTGAAAAACTCTACGAAGAACTCTTGGTA
TCAACCGAACTCGTTGACAATCAAGTTATGGATAAGATTTTCGTTGGTAAGGTTAATGTCATGCCTTTAGAATCCATCAA
TCAAAAGATTAGAGAGTTCCGCACTCTCAGTGGAGATGAGTTGAAGCAAGCTATTATCGCCTTTGCTAATCAAACAACCC
ACATTGAATAA

Upstream 100 bases:

>100_bases
ACAAGGGAACAATAATTTATTTGGATAAAATAAGCCAAATAAATAAAAATATGCTATAATAACAATATAGTAAAACTCTT
TTAAGGAGGAGTAGATTTCT

Downstream 100 bases:

>100_bases
AAAAGAAAAACGCATATTACCAAGTTAAACAACCTTGGTAATATGCGTTTTATTATGTAGAGACTTATACTCTTCGAAAA
TCTCTTCAAACCACGTCAAC

Product: capsular polysaccharide biosynthesis protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASIS
RYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERI
VLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYN
VAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLV
STELVDNQVMDKIFVGKVNVMPLESINQKIREFRTLSGDELKQAIIAFANQTTHIE

Sequences:

>Translated_616_residues
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASIS
RYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERI
VLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYN
VAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLV
STELVDNQVMDKIFVGKVNVMPLESINQKIREFRTLSGDELKQAIIAFANQTTHIE
>Mature_616_residues
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASIS
RYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERI
VLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYN
VAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLV
STELVDNQVMDKIFVGKVNVMPLESINQKIREFRTLSGDELKQAIIAFANQTTHIE

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 69307; Mature: 69307

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTS
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIIL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGAGDGGALFMDSYQHPTSELEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEE
VGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
EEEECCCCHHCCCCCCCCCEECCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHH
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGA
CCCEEEECCCHHHHHHHHHHHCCCCEEECHHHHHCCHHHCCCHHHCCCEECCCEEEEECC
GGSIGSEICRQVSRFNPERIVLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLL
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCEEHHHCHHHHHHHH
QVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAKAVDEAKVSKMVMISTDK
HHHHHCCCEEEEEEHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCC
AVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCEEHHHHHHHCCCCCEE
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESE
EEEEEEEEHEEECCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHEEECCCCCC
IPIVEVGIRPGEKLYEELLVSTELVDNQVMDKIFVGKVNVMPLESINQKIREFRTLSGDE
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCHHH
LKQAIIAFANQTTHIE
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTS
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIIL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGAGDGGALFMDSYQHPTSELEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEE
VGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
EEEECCCCHHCCCCCCCCCEECCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHH
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGA
CCCEEEECCCHHHHHHHHHHHCCCCEEECHHHHHCCHHHCCCHHHCCCEECCCEEEEECC
GGSIGSEICRQVSRFNPERIVLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLL
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCEEHHHCHHHHHHHH
QVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAKAVDEAKVSKMVMISTDK
HHHHHCCCEEEEEEHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCC
AVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCEEHHHHHHHCCCCCEE
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESE
EEEEEEEEHEEECCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHEEECCCCCC
IPIVEVGIRPGEKLYEELLVSTELVDNQVMDKIFVGKVNVMPLESINQKIREFRTLSGDE
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCHHH
LKQAIIAFANQTTHIE
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]