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The map label for this gene is glgB

Identifier: 116517137

GI number: 116517137

Start: 1017799

End: 1019727

Strand: Direct

Name: glgB

Synonym: SPD_1005

Alternate gene names: 116517137

Gene position: 1017799-1019727 (Clockwise)

Preceding gene: 116516523

Following gene: 116516326

Centisome position: 49.74

GC content: 40.69

Gene sequence:

>1929_bases
ATGGATAATAGAGAAGCATTAAAAACCTTTATGACGGGTGAAAATTTTTATCTCCAACATTATCTAGGAGCACATAGGGA
AGAACTAAATGGAGAGCATGGCTATACCTTCCGTGTTTGGGCACCTAATGCTCAGGCTGTTCACTTGGTTGGTGATTTTA
CCAACTGGATTGAAAATCAGATTCCAATGGTAAGAAATGATTTTGGGGTCTGGGAAGTCTTTACCAATATGGCTCAAGAA
GGGCATATTTACAAATATCATGTCACACGTCAAAATGGTCATCAACTGATGAAGATTGACCCTTTTGCTGTCAGGTATGA
GGCTCGTCCAGGAACAGGGGCAATCGTAACAGAGCTTCCTGAGAAGAAATGGAAGGATGGACTTTGGCTGGCACGAAGAA
AACGTTGGGGCTTTGCTGAACGTCCTGTCAATATTTACGAGGTTCATGCTGGCTCTTGGAAGAGAAATCCTGATGGCAGT
CCTTATAGTTTTGCCCAATTGAAAGATGAACTCATTCCTTACCTAGTTGAGATGAACTATACTCATATTGAGTTTATGCC
CTTGATGTCCCACCCACTAGGTTTGAGTTGGGGGTACCAGCTTATGGGTTACTTCGCTTTAGAGCATGCCTATGGTAGAC
CAGAGGAGTTTCAAGATTTTGTCGAGGAGTGTCATACCCATAATATTGGGGTTATTGTGGACTGGGTACCAGGTCACTTT
ACCATCAATGATGATGCCTTAGCCTATTATGATGGGACACCGACTTTTGAATACCAAGACCATAATAAGGCTCATAACCA
TGGTTGGGGTGCCCTTAATTTTGACCTTGGAAAAAATGAAGTCCAGTCCTTCTTAATTTCTTGCATTAAGCATTGGATTG
ATGTCTATCATTTGGATGGTATTCGTGTGGATGCTGTTAGCAACATGCTCTATTTGGACTATGATGATGCTCCATGGACA
CCTAATAAAGATGGCGGAAATCTCAACTATGAAGGTTATTATTTCCTTCAACGCTTGAATGAAGTTATTAAGTTAGAATA
TCCAGATGTGATGATGATTGCAGAAGAAAGTTCGTCTGCAACCAAGATTACGGGAATGAAAGAGATTGGTGGTCTAGGAT
TTGACTACAAATGGAACATGGGCTGGATGAATGATATCCTCCGTTTCTACGAAGAAGATCCGATTTATCGTAAATATGAC
TTTAACCTGGTGACTTTCAGCTTTATGTATGTTTTCAAGGAGAATTATCTCTTGCCATTCTCGCACGATGAAGTGGTTCA
TGGCAAGAAGAGTATGATGCATAAGATGTGGGGAGATCGTTACAATCAATTCGCAGGCTTGCGCAATCTCTATACGTACC
AAATTTGTCACCCTGGTAAGAAATTGCTCTTCATGGGTAGCGAATACGGTCAATTCCTAGAATGGAAATCTGAAGAACAG
TTGGAATGGTCTAACCTAGAAGACCCAATGAATGCTAAGATGAAGTATTTCACTTCTCAGCTAAACCAGTTTTACAAAGA
TCATCGCTGTCTGTGGGAAATCGATACCAGCTATGATGGTATTGAAATCATTGATGCGGATAATCGAGACCAGAGTGTTC
TTTCCTTTATTCGTAAGGGTAAAAAGGGAGAAATGTTAGTCTGTATCTTTAACATGGTACCTGTTGAGCGGAAAGATTTT
ACAATCGGACTACCCGTTGCAGGAATTTACGAAGAAGTATGGAATACTGAGTTGGAAGAGTGGGGAGGCGTTTGGAAAGA
ACATAATCAAACTGTTCAAACGCAAGAAGGACTATGGAAAGATTATGAGCAGACCTTAACCTTTACCCTACCGGCTATGG
GAGCAAGTGTATGGAAAATCAAACGTCGCTTGAAATCTACTAAAACCGTCACAAATAAAAACCCAAAAGGAGTAGAAAAT
GAAAAATGA

Upstream 100 bases:

>100_bases
TTTTCAAAATAATTAAAGAAGTATAGTAATTTACTTGAATCTTCTCTAAAAAGGTAGTATAATAAAAACATAGAAAACGC
TTACAGGAGAAGGGGAGTAT

Downstream 100 bases:

>100_bases
AATGTTAGCTTTGATTCTTGCTGGTGGGCAAGGAACTCGTCTCGGTAAACTCACTCAAAGCATCGCAAAACCAGCTGTGC
AATTTGGTGGGCGCTACCGT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNAQAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQE
GHIYKYHVTRQNGHQLMKIDPFAVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQDFVEECHTHNIGVIVDWVPGHF
TINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDLGKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWT
PNKDGGNLNYEGYYFLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEEDPIYRKYD
FNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYTYQICHPGKKLLFMGSEYGQFLEWKSEEQ
LEWSNLEDPMNAKMKYFTSQLNQFYKDHRCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDF
TIGLPVAGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRLKSTKTVTNKNPKGVEN
EK

Sequences:

>Translated_642_residues
MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNAQAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQE
GHIYKYHVTRQNGHQLMKIDPFAVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQDFVEECHTHNIGVIVDWVPGHF
TINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDLGKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWT
PNKDGGNLNYEGYYFLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEEDPIYRKYD
FNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYTYQICHPGKKLLFMGSEYGQFLEWKSEEQ
LEWSNLEDPMNAKMKYFTSQLNQFYKDHRCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDF
TIGLPVAGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRLKSTKTVTNKNPKGVEN
EK
>Mature_642_residues
MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNAQAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQE
GHIYKYHVTRQNGHQLMKIDPFAVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQDFVEECHTHNIGVIVDWVPGHF
TINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDLGKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWT
PNKDGGNLNYEGYYFLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEEDPIYRKYD
FNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYTYQICHPGKKLLFMGSEYGQFLEWKSEEQ
LEWSNLEDPMNAKMKYFTSQLNQFYKDHRCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDF
TIGLPVAGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRLKSTKTVTNKNPKGVEN
EK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=615, Percent_Identity=26.1788617886179, Blast_Score=156, Evalue=7e-38,
Organism=Escherichia coli, GI1789839, Length=625, Percent_Identity=37.28, Blast_Score=453, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17554896, Length=591, Percent_Identity=24.7038917089679, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32564391, Length=576, Percent_Identity=23.9583333333333, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320826, Length=654, Percent_Identity=24.1590214067278, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28573410, Length=618, Percent_Identity=25.5663430420712, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_STRP2 (Q04KG8)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816480.1
- ProteinModelPortal:   Q04KG8
- STRING:   Q04KG8
- EnsemblBacteria:   EBSTRT00000020140
- GeneID:   4442375
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1005
- eggNOG:   COG0296
- GeneTree:   EBGT00050000026664
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK12313
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 75543; Mature: 75543

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: ACT_SITE 199-199 ACT_SITE 234-234 ACT_SITE 239-239 ACT_SITE 302-302 ACT_SITE 304-304 ACT_SITE 355-355 ACT_SITE 422-422 ACT_SITE 423-423

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNAQAVHLVGDFTNWIENQ
CCCHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHC
IPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPFAVRYEARPGTGAIVTELP
CCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCEEEEEECCCCCCCEEECCC
EKKWKDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGSPYSFAQLKDELIPYLVEMNY
HHHCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEECCC
THIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQDFVEECHTHNIGVIVDWVPGHF
CEEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCEE
TINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDLGKNEVQSFLISCIKHWIDVYHLDG
EECCCEEEEECCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEECC
IRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYYFLQRLNEVIKLEYPDVMMIAEESSSA
EEEEEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCEEEEEECCCCC
TKITGMKEIGGLGFDYKWNMGWMNDILRFYEEDPIYRKYDFNLVTFSFMYVFKENYLLPF
EEECCHHHHCCCCCCEEECCCHHHHHHHHHCCCCCEEEECCCCHHHHHEEHHHCCEEECC
SHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYTYQICHPGKKLLFMGSEYGQFLEWKSEEQ
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCHHHHHCCCCCCC
LEWSNLEDPMNAKMKYFTSQLNQFYKDHRCLWEIDTSYDGIEIIDADNRDQSVLSFIRKG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHCC
KKGEMLVCIFNMVPVERKDFTIGLPVAGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWK
CCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHCCCHHHHCCHHHHCCCCCHHHHHHHH
DYEQTLTFTLPAMGASVWKIKRRLKSTKTVTNKNPKGVENEK
HHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNAQAVHLVGDFTNWIENQ
CCCHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHC
IPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPFAVRYEARPGTGAIVTELP
CCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCEEEEEECCCCCCCEEECCC
EKKWKDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGSPYSFAQLKDELIPYLVEMNY
HHHCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEECCC
THIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQDFVEECHTHNIGVIVDWVPGHF
CEEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCEE
TINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDLGKNEVQSFLISCIKHWIDVYHLDG
EECCCEEEEECCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEECC
IRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYYFLQRLNEVIKLEYPDVMMIAEESSSA
EEEEEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCEEEEEECCCCC
TKITGMKEIGGLGFDYKWNMGWMNDILRFYEEDPIYRKYDFNLVTFSFMYVFKENYLLPF
EEECCHHHHCCCCCCEEECCCHHHHHHHHHCCCCCEEEECCCCHHHHHEEHHHCCEEECC
SHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYTYQICHPGKKLLFMGSEYGQFLEWKSEEQ
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCHHHHHCCCCCCC
LEWSNLEDPMNAKMKYFTSQLNQFYKDHRCLWEIDTSYDGIEIIDADNRDQSVLSFIRKG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHCC
KKGEMLVCIFNMVPVERKDFTIGLPVAGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWK
CCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHCCCHHHHCCHHHHCCCCCHHHHHHHH
DYEQTLTFTLPAMGASVWKIKRRLKSTKTVTNKNPKGVENEK
HHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA