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The map label for this gene is tenA [H]

Identifier: 116517113

GI number: 116517113

Start: 649724

End: 650416

Strand: Direct

Name: tenA [H]

Synonym: SPD_0628

Alternate gene names: 116517113

Gene position: 649724-650416 (Clockwise)

Preceding gene: 116516329

Following gene: 116516704

Centisome position: 31.75

GC content: 39.68

Gene sequence:

>693_bases
ATGGAATTTACAGATATTGCGATGGAATTATCCAAGAAAGCTTGGCAGGCTTCCTTTCATCATCCCTTTATTTTACAATT
ACAAGAGGGGAATTTAGAACCTGCCATTTTCCGCTATTACCTGATTCAGGATGCCTACTATCTGAAGGCCTTCTCAGAAA
TCTATCATCTTTTGGCTGATAAGACTTCAAACCAAGAGATGAAAAGACTCTTGAAACAAAATGCTCAGGGTTTAGTGGAG
GGTGAGTTATTTATTCGCCAACAATTTTTCAAGGAATTGGAAATCAGCGACCAGGAAATGGAGCAACATCCAATCGCTCC
AACCTGTTATCATTACATTTCTCATATTTATAGGCAATTTGCAGAACCGAACTTAGCCATCGCTTTTGCAAGCTTGCTTC
CTTGTCCTTGGTTATACCATGATATAGGCAAATCACTTAATCTTAAACCATCACCAAATCCTCTCTATCAACAATGGATT
GAAACTTATATTACGGATGAGTTAGAGCAACAGATCAGAGAGGAGGGAGCACTGGTCAATCAGCTCTATCGAGAAAGTGA
CGAGACAGACAAGCAAAAAATGCTAGATACCTTCCACATCAGTGTTCATATGGAAGCCAAGTTTTGGGAAATGGCCTACC
AACACCAGACATGGAAGAGCGATTTACAGTCTTTAGAAAAAGGAGAAGAATAG

Upstream 100 bases:

>100_bases
AATAGCTATCGGACTTTTTATATTCCTAAAAAAACAAAATTAATCTACCTGCTTTTCTTTTTATTGCTTCAAACCAGTCT
ATTTTTATAAAGGAGTTATT

Downstream 100 bases:

>100_bases
AAGCATGAGAAAGCACCAATTACAAGTTCACAAATTAACCATTTTATCTATGATGATTGCCCTTGATGTAGTCCTTACAC
CTATCTTTCGAATTGAGGGA

Product: transcriptional activator TenA, TENA/THI-4 family protein

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQDAYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVE
GELFIRQQFFKELEISDQEMEQHPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDTFHISVHMEAKFWEMAYQHQTWKSDLQSLEKGEE

Sequences:

>Translated_230_residues
MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQDAYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVE
GELFIRQQFFKELEISDQEMEQHPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDTFHISVHMEAKFWEMAYQHQTWKSDLQSLEKGEE
>Mature_230_residues
MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQDAYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVE
GELFIRQQFFKELEISDQEMEQHPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDTFHISVHMEAKFWEMAYQHQTWKSDLQSLEKGEE

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 27374; Mature: 27374

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQDAYYLKAFSEIYHLLAD
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQHPIAPTCYHYISHIYRQF
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHH
AEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWIETYITDELEQQIREEGALVN
CCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYRESDETDKQKMLDTFHISVHMEAKFWEMAYQHQTWKSDLQSLEKGEE
HHHHCCCCHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQDAYYLKAFSEIYHLLAD
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQHPIAPTCYHYISHIYRQF
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHH
AEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWIETYITDELEQQIREEGALVN
CCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYRESDETDKQKMLDTFHISVHMEAKFWEMAYQHQTWKSDLQSLEKGEE
HHHHCCCCHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]