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The map label for this gene is ispD

Identifier: 116517103

GI number: 116517103

Start: 1157851

End: 1158558

Strand: Reverse

Name: ispD

Synonym: SPD_1127

Alternate gene names: 116517103

Gene position: 1158558-1157851 (Counterclockwise)

Preceding gene: 116516501

Following gene: 116516315

Centisome position: 56.62

GC content: 38.84

Gene sequence:

>708_bases
ATGATTTATGCAGGAATTCTTGCCGGTGGAACTGGCACACGCATGGGGATCAGTAACTTGCCAAAACAATTTTTAGAGCT
AGGTGATCGACCTATTTTGATTCATACAATTGAAAAATTTGTCTTGGAGCCAAGTATTGAAAAAATTGTAGTTGGTGTTC
ATGGAGACTGGGTTTCTCATGCAGAAGATCTTGTAGATAAATATCTTCCTCTTTATAAGGAACGTATCATCATTACAAAG
GGTGGTGCTGACCGCAATACAAGTATTAAGAACATCATTGAAGCCATTGATGCTTATCGTCCGCTTACTCCAGAGGATAT
CGTTGTTACCCACGATTCTGTTCGTCCATTTATTACACTTCGCATGATTCAGGACAATATCCAACTTGCCCAAAATCATG
ACGCAGTGGACACAGTGGTAGAAGCGGTTGATACTATCGTTGAAAGTACCAATGGTCAATTTATTACAGATATTCCAAAT
CGTGCTCACCTTTATCAAGGACAAACACCTCAAACATTCCGTTGCAAGGACTTCATGGACCTTTATGGATCTCTTTCTGA
TGAAGAGAAGGAAATCTTGACAGATGCATGTAAAATCTTTGTGATCAAAGGAAAAGATGTGGCTTTGGCCAAAGGTGAAT
ACTCAAATCTGAAGATTACAACCGTAACAGATTTGAAGATTGCAAAAAGTATGATTGAGAAAGACTAG

Upstream 100 bases:

>100_bases
CTATTCTAGAAGGGGAGGACCAGTATTTCTTTTATGATAGGACTAGATTGTGGTATAATAGAGAGAATAAGTTTTTTTAG
TAAGACAAAGGAGAAAATAG

Downstream 100 bases:

>100_bases
TAAAATGATTAATCAAATTTATCAACTAACTAAGCCTAAGTTTATCAATGTCAAATATCAGGAAGAGGCTATTGACCAAG
AGAATCATATCCTTATCCGT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITK
GGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD

Sequences:

>Translated_235_residues
MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITK
GGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD
>Mature_235_residues
MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITK
GGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=28.3333333333333, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI157671913, Length=202, Percent_Identity=26.7326732673267, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_STRP2 (Q04K52)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816596.1
- ProteinModelPortal:   Q04K52
- SMR:   Q04K52
- STRING:   Q04K52
- EnsemblBacteria:   EBSTRT00000019995
- GeneID:   4441914
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1127
- eggNOG:   COG1211
- GeneTree:   EBGT00050000027822
- HOGENOM:   HBG672839
- OMA:   GELFNIK
- ProtClustDB:   PRK00155
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26243; Mature: 26243

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPSIEKIVVGVHGDWVSH
CEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCHHEEEEEECCCHHHH
AEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITL
HHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEE
RMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTPQTFRCKDFMD
EEHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCHHHHHH
LYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD
HHCCCCCHHHHHHHHHHEEEEECCCCEEEECCCCCCEEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPSIEKIVVGVHGDWVSH
CEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCHHEEEEEECCCHHHH
AEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITL
HHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEE
RMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTPQTFRCKDFMD
EEHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCHHHHHH
LYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD
HHCCCCCHHHHHHHHHHEEEEECCCCEEEECCCCCCEEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA