Click here to switch to the map view.

The map label for this gene is nfrA2 [H]

Identifier: 116517075

GI number: 116517075

Start: 1543012

End: 1543725

Strand: Reverse

Name: nfrA2 [H]

Synonym: SPD_1520

Alternate gene names: 116517075

Gene position: 1543725-1543012 (Counterclockwise)

Preceding gene: 116516206

Following gene: 116515879

Centisome position: 75.45

GC content: 45.24

Gene sequence:

>714_bases
ATGACAGAAACCATTAAATTGATGAAGGCTCATACTTCAGTGCGCAGGTTTAAAGAGCAAGAAATTCCCCAAGTAGACTT
AAATGAGATTTTGACAGCAGCCCAGATGGCATCATCTTGGAAGAATTTCCAATCCTACTCTGTGATTGTGGTACGAAGTC
AAGAGAAGAAAGATGCCTTGTATGAATTGGTACCTCAAGAAGCCATTCGCCAGTCTGCTGTTTTCCTTCTCTTTGTCGGA
GATTTGAACCGAGCAGAAAAGGGAGCCCGACTTCATACCGACACCTTCCAACCCCAAGGTGTAGAAGGTCTCTTGATTAG
TTCGGTGGATGCGGCTTTGGCTGGACAAAATGCCCTGCTGGCAGCTGAAAGTTTGGGTTATGGTGGTGTGATTATTGGCT
TGGTACGGTACAAGTCAGAAGAAGTGGCAGAACTCTTTAACCTGCCTGACTACACCTATCCTGTCTTTGGGATGGCACTA
GGTGTTCCAAATCAAACTCATGATGTGAAACCAAGACTGCCACTAGATAATGTAGTCTTTGAGGAAGAATACCAAGAACA
GTCAACTGAGGCAATCCAAGCTTATGACCGTGTTCAGGCTGACTATGCTGGGGCGCGTGCGACCACAAGCTGGAGTCAGC
GTCTAGCAGAACAGTTTGGTCAAGCTGAACCAAGCTCAACTAGAAAAAATCTTGAACAGAAGAAATTATTGTAG

Upstream 100 bases:

>100_bases
AAGTGAAACATGGAAATGACGAAACCTGGGAAGCCAGACGGGTCATGGAACGCATCCGTTATTTGGCTGAGGAGACTCGT
TTAGAAGGAGTAAACCGTCG

Downstream 100 bases:

>100_bases
AAAGTGAGAAATTATGGCCCTACCAACTATTGCCATTGTAGGACGTCCCAATGTTGGGAAATCAACCCTATTTAATCGGA
TCGCTGGTGAGCGAATCTCC

Product: nitroreductase family protein

Products: NA

Alternate protein names: NAD(P)H-dependent FMN reductase; NAD(P)H-dependent nitroreductase; NAD(P)H-dependent oxidoreductase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVG
DLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL

Sequences:

>Translated_237_residues
MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVG
DLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL
>Mature_236_residues
TETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGD
LNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALG
VPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=220, Percent_Identity=36.3636363636364, Blast_Score=121, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 26472; Mature: 26341

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDAL
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
YELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALL
HHHCCHHHHHHHHEEEEEECCCCCHHCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCEE
AAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALGVPNQTHDVKPRLPLDNVVF
EHHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHH
EEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDAL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
YELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALL
HHHCCHHHHHHHHEEEEEECCCCCHHCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCEE
AAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALGVPNQTHDVKPRLPLDNVVF
EHHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHH
EEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]