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The map label for this gene is grpE

Identifier: 116517069

GI number: 116517069

Start: 468584

End: 469108

Strand: Direct

Name: grpE

Synonym: SPD_0459

Alternate gene names: 116517069

Gene position: 468584-469108 (Clockwise)

Preceding gene: 116516738

Following gene: 116516498

Centisome position: 22.9

GC content: 43.81

Gene sequence:

>525_bases
ATGGCCCAAGATATAAAAAATGAAGAAGTAGAAGAAGTTCAAGAAGAGGAAGTTGTGGAAACAGCTGAAGAAACAACTCC
TGAAAAGTCTGAGTTGGACTTGGCAAATGAACGTGCAGATGAGTTCGAAAACAAATATCTTCGCGCTCATGCAGAAATGC
AAAATATCCAACGCCGTGCCAATGAAGAACGTCAAAACTTGCAACGTTATCGTAGCCAGGACTTGGCAAAAGCAATCTTA
CCATCTCTTGACAACCTTGAGCGTGCACTTGCAGTTGAAGGTTTGACAGATGATGTGAAGAAGGGCTTGGCGATGGTGCA
AGAAAGCTTGATTCACGCTTTGAAAGAAGAAGGAATTGAAGAAATCGCAGCAGATGGCGAATTTGACCATAACTACCATA
TGGCCATCCAAACTCTCCCAGGAGACGATGAACACCCAGTAGATACCATCGCCCAAGTCTTTCAAAAAGGCTACAAACTC
CATGACCGCATCCTACGCCCAGCAATGGTAGTGGTGTATAACTAA

Upstream 100 bases:

>100_bases
TCAACCGTGTTTTGACCATGAAGTTGACAGATTTTTACCGCTACCTCAGCAGTAATCATTACGAAGTACATTAAGATTGA
AATCATTAAAGGAGGCGAAC

Downstream 100 bases:

>100_bases
GATACAAAGCTCGTAAAAAGCTCGCAGTAAAAATAGGAGATTGACGAAGTGTTCGATGAACACAAGAAAATCTATCTTTT
TTACTCAGAGCTTAGGGCGT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFENKYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAIL
PSLDNLERALAVEGLTDDVKKGLAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPGDDEHPVDTIAQVFQKGYKL
HDRILRPAMVVVYN

Sequences:

>Translated_174_residues
MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFENKYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAIL
PSLDNLERALAVEGLTDDVKKGLAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPGDDEHPVDTIAQVFQKGYKL
HDRILRPAMVVVYN
>Mature_173_residues
AQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFENKYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILP
SLDNLERALAVEGLTDDVKKGLAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPGDDEHPVDTIAQVFQKGYKLH
DRILRPAMVVVYN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=132, Percent_Identity=31.0606060606061, Blast_Score=68, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=178, Percent_Identity=32.0224719101124, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653432, Length=171, Percent_Identity=30.4093567251462, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_STRP2 (Q04LY1)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815967.1
- ProteinModelPortal:   Q04LY1
- SMR:   Q04LY1
- STRING:   Q04LY1
- EnsemblBacteria:   EBSTRT00000019898
- GeneID:   4441918
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0459
- eggNOG:   COG0576
- GeneTree:   EBGT00050000028385
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14156
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 19969; Mature: 19838

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFENKYLRAHAEMQNIQRRA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGLAMVQESLIHALKEEGIE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EIAADGEFDHNYHMAIQTLPGDDEHPVDTIAQVFQKGYKLHDRILRPAMVVVYN
HHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
AQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFENKYLRAHAEMQNIQRRA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGLAMVQESLIHALKEEGIE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EIAADGEFDHNYHMAIQTLPGDDEHPVDTIAQVFQKGYKLHDRILRPAMVVVYN
HHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA