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The map label for this gene is gltX

Identifier: 116516946

GI number: 116516946

Start: 1865079

End: 1866539

Strand: Reverse

Name: gltX

Synonym: SPD_1896

Alternate gene names: 116516946

Gene position: 1866539-1865079 (Counterclockwise)

Preceding gene: 116516610

Following gene: 116515341

Centisome position: 91.22

GC content: 40.86

Gene sequence:

>1461_bases
ATGTCAAAAGATATCCGCGTACGTTACGCACCAAGTCCAACAGGACTACTACACATCGGAAATGCTCGTACAGCATTGTT
TAATTACTTGTATGCGCGCCATCATGGTGGAACATTTCTCATCCGTATCGAAGATACTGACCGTAAACGCCATGTCGAGG
ATGGTGAACGTTCACAACTTGAAAACCTTCGCTGGTTAGGCATGGATTGGGATGAAAGTCCAGAATCACATGAGAATTAT
CGCCAGTCTGAGCGTTTGGACTTGTATCAAAAATACATTGACCAACTATTAGCTGAAGGAAAAGCCTATAAATCTTACGT
TACAGAAGAAGAGTTGGCAGCTGAACGCGAACGCCAAGAAGTAGCTGGCGAAACACCACGCTACATCAATGAATACCTTG
GTATGAGTGAAGAAGAAAAAGCAGCTTACATCGCAGAACGTGAAGCAGCAGGGATCATCCCAACTGTTCGTTTGGCTGTC
AATGAGTCAGGTATCTACAAGTGGCATGATATGGTCAAAGGCGATATCGAATTTGAAGGTGGCAATATCGGTGGTGACTG
GATTATCCAAAAGAAAGACGGTTACCCAACTTACAACTTTGCCGTTGCCATCGATGACCATGATATGCAAATCTCTCATG
TTATCCGTGGAGACGACCATATTGCTAACACACCAAAACAGCTCATGGTTTATGAAGCACTTGGTTGGGAAGCTCCAGAG
TTTGGTCACATGACCTTGATTATCAACTCTGAGACTGGTAAGAAGTTATCTAAACGTGATACTAATACACTTCAATTTAT
CGAAGATTACCGAAAGAAAGGTTACCTTCCAGAAGCAGTCTTTAACTTTATTGCTCTTCTTGGTTGGAACCCAGGTGGTG
AAGATGAGATTTTCTCTCGTGAAGAACTCATTAAACTTTTTGATGAAAACCGCCTCAGCAAGTCACCAGCAGCCTTTGAT
CAGAAAAAACTAGACTGGATGAGCAATGATTATATCAAGAATGCAGACCTTGAAACCATCTTTGAAATGGCAAAACCATT
CTTAGAGGAAGCAGGCCGTTTGACTGACAAGGCTGAAAAATTAGTTGAGCTCTATAAACCACAAATGAAATCAGTAGATG
AGATTATCCCATTGACAGATCTTTTCTTCTCAGATTTCCCAGAATTGACAGAAGCAGAGCGCGAAGTCATGACGGGTGAA
ACAGTTCCAACAGTTCTTGAAGCATTCAAAGCAAAACTTGAAGCGATGACAGATGATAAATTTGTGACAGAAAATATCTT
CCCACAAATTAAAGCAGTTCAAAAAGAAACAGGTATTAAAGGGAAAAATCTTTTCATGCCTATTCGTATCGCAGTTTCAG
GCGAAATGCATGGGCCAGAATTACCAGATACAATTTTCTTGCTTGGACGTGAAAAATCAATTCAGCATATCGAAAACATG
CTAAAAGAAATCTCTAAATAA

Upstream 100 bases:

>100_bases
ACTCTTTTTACTCTCTTTATCCATAGAAATTGGACTCAGCCAAGACTTGTGATATAATATAGAAAGCAAAAAGGCAGACG
CCTAGATAATAGGAGAAACT

Downstream 100 bases:

>100_bases
AGAGGATACAATAATGGACATCTGGGAAAAGATGTACGAAGAAGCACAGAAACTATACAATCCACATGAAGTATCAGACT
TTGTTTATGCCAATCATGTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQLENLRWLGMDWDESPESHENY
RQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFD
QKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGE
TVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENM
LKEISK

Sequences:

>Translated_486_residues
MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQLENLRWLGMDWDESPESHENY
RQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFD
QKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGE
TVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENM
LKEISK
>Mature_485_residues
SKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYR
QSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAVN
ESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPEF
GHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQ
KKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGET
VPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENML
KEISK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=29.5180722891566, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI62241042, Length=258, Percent_Identity=26.7441860465116, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4826960, Length=298, Percent_Identity=24.8322147651007, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788743, Length=485, Percent_Identity=38.3505154639175, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=30.0395256916996, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786895, Length=217, Percent_Identity=25.8064516129032, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=499, Percent_Identity=30.2605210420842, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17543332, Length=298, Percent_Identity=25.8389261744966, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=509, Percent_Identity=31.8271119842829, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=218, Percent_Identity=27.5229357798165, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=310, Percent_Identity=24.1935483870968, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=30.2186878727634, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17864488, Length=300, Percent_Identity=27.6666666666667, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_STRP2 (Q04IA3)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817285.1
- ProteinModelPortal:   Q04IA3
- SMR:   Q04IA3
- STRING:   Q04IA3
- EnsemblBacteria:   EBSTRT00000018981
- GeneID:   4442022
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1896
- eggNOG:   COG0008
- GeneTree:   EBGT00050000027201
- HOGENOM:   HBG628189
- OMA:   YRCFTSE
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55913; Mature: 55782

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQL
CCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQE
HCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
VAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAVNESGIYKWHDMVKGDIEFEG
HCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCEEEECC
GNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
CCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCHHCCCHHHHHEEHHHCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHCH
EELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGETVPTVLEAFKAKLEAMTDDK
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
FVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENM
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHH
LKEISK
HHHHCC
>Mature Secondary Structure 
SKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQL
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQE
HCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
VAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAVNESGIYKWHDMVKGDIEFEG
HCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCEEEECC
GNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
CCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCHHCCCHHHHHEEHHHCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHCH
EELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGETVPTVLEAFKAKLEAMTDDK
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
FVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENM
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHH
LKEISK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA