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The map label for this gene is pheS

Identifier: 116516862

GI number: 116516862

Start: 513117

End: 514163

Strand: Direct

Name: pheS

Synonym: SPD_0504

Alternate gene names: 116516862

Gene position: 513117-514163 (Clockwise)

Preceding gene: 116515964

Following gene: 116516224

Centisome position: 25.08

GC content: 45.37

Gene sequence:

>1047_bases
ATGTCAACTATTGAAGAACAATTAAAAGCGCTTCGCGAAGAAACGCTGACTAGCTTGAAGCAGATTACTGCTGGAAATGA
AAAAGAGATGCAAGATTTGCGTGTCTCTGTCCTTGGTAAAAAGGGTTCGCTCACTGAAATCCTCAAAGGGATGAAAGATG
TTTCTGCTGAGATGCGTCCAATCATCGGGAAACACGTCAATGAAGCTCGTGATGTCTTGACAGCTGCTTTTGAAGAAACA
GCTAAGCTCTTGGAAGAAAAGAAAGTCGCGGCTCAACTGGCTAGCGAGAGTATCGATGTGACGCTTCCAGGTCGTCCAGT
TGCGACTGGTCACCGTCACGTTTTGACACAAACCAGTGAAGAAATCGAAGATATCTTCATCGGTATGGGTTATCAAGTCG
TGGATGGTTTTGAAGTGGAGCAAGACTACTATAACTTTGAACGTATGAACCTTCCAAAAGACCACCCAGCTCGTGATATG
CAGGATACTTTCTATATCACTGAAGAAATCTTGCTCCGTACCCACACGTCTCCAGTTCAGGCGCGTGCTATGGATGCCCA
TGATTTTTCTAAAGGTCCTTTGAAGATGATCTCGCCAGGGCGTGTCTTCCGTCGCGATACGGACGATGCGACCCACAGTC
ACCAATTCCACCAAATCGAAGGCTTGGTAGTTGGGAAAAATATCTCTATGGCTGATCTTCAAGGAACGCTTCAGTTGATT
GTCCAAAAAATGTTTGGTGAAGAGCGTCAAATTCGTTTGCGTCCATCTTACTTCCCATTCACAGAGCCATCTGTTGAGGT
GGATGTTTCTTGCTTCAAGTGTGGTGGAGAAGGCTGTAACGTATGTAAGAAAACAGGTTGGATCGAAATTATGGGAGCCG
GTATGGTTCACCCACGTGTCCTTGAAATGAGTGGTATCGATGCGACTGTATACTCTGGTTTTGCCTTTGGTCTTGGACAA
GAGCGTGTAGCTATGCTCCGTTATGGAATCAACGATATCCGTGGATTCTACCAAGGAGATGTCCGCTTCTCAGAACAGTT
TAAATAA

Upstream 100 bases:

>100_bases
ACTTGTCAGATGAAAACGGATGGTACCGCGTGTCAACGCTCCGAGTGGAGTTTTTGGCATGTGGTTTTCTTTTTATCTAC
GAGAGACTGATGGAGGAAAT

Downstream 100 bases:

>100_bases
TGATTAGAAAAGTAGAAATGGCAGATGTTGAGGTGTTGGCTAAAATTGCCAAACAAGCCTTTCGTGAAACCTTTGCATAT
GATAATACGGAAGAGCAGTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSLTEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEET
AKLLEEKKVAAQLASESIDVTLPGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMADLQGTLQLI
VQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGGEGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQ
ERVAMLRYGINDIRGFYQGDVRFSEQFK

Sequences:

>Translated_348_residues
MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSLTEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEET
AKLLEEKKVAAQLASESIDVTLPGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMADLQGTLQLI
VQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGGEGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQ
ERVAMLRYGINDIRGFYQGDVRFSEQFK
>Mature_347_residues
STIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSLTEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETA
KLLEEKKVAAQLASESIDVTLPGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDMQ
DTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMADLQGTLQLIV
QKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGGEGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQE
RVAMLRYGINDIRGFYQGDVRFSEQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=33.0855018587361, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI5729820, Length=292, Percent_Identity=29.1095890410959, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=46.4615384615385, Blast_Score=301, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=31.5018315018315, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=31.5018315018315, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI32566635, Length=246, Percent_Identity=32.1138211382114, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=274, Percent_Identity=30.2919708029197, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=282, Percent_Identity=29.0780141843972, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18858079, Length=286, Percent_Identity=30.7692307692308, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17137424, Length=251, Percent_Identity=27.8884462151394, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_STRP2 (Q04LU1)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816007.1
- ProteinModelPortal:   Q04LU1
- SMR:   Q04LU1
- STRING:   Q04LU1
- PhosSite:   Q04LU1
- EnsemblBacteria:   EBSTRT00000019114
- GeneID:   4442144
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0504
- eggNOG:   COG0016
- GeneTree:   EBGT00050000028256
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39132; Mature: 39001

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSLTEILKGMKDVSAEMRP
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTLPGRPVATGHRHVLTQTSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCHH
EIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDMQDTFYITEEILLRTHTSPVQ
HHHHHHHCCCEEEECCEEECHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
ARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMADLQGTLQLI
HHHCCCCCCCCCCEEECCCCHHEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHH
VQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGGEGCNVCKKTGWIEIMGAGMVHPRV
HHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCCEEEEECCCCCCEE
LEMSGIDATVYSGFAFGLGQERVAMLRYGINDIRGFYQGDVRFSEQFK
EEECCCCHHHHCCHHHCCCHHHHHHHHHCHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
STIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSLTEILKGMKDVSAEMRP
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTLPGRPVATGHRHVLTQTSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCHH
EIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDMQDTFYITEEILLRTHTSPVQ
HHHHHHHCCCEEEECCEEECHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
ARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMADLQGTLQLI
HHHCCCCCCCCCCEEECCCCHHEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHH
VQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGGEGCNVCKKTGWIEIMGAGMVHPRV
HHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCCEEEEECCCCCCEE
LEMSGIDATVYSGFAFGLGQERVAMLRYGINDIRGFYQGDVRFSEQFK
EEECCCCHHHHCCHHHCCCHHHHHHHHHCHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA