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The map label for this gene is murA-2 [H]

Identifier: 116516843

GI number: 116516843

Start: 1762452

End: 1763735

Strand: Reverse

Name: murA-2 [H]

Synonym: SPD_1764

Alternate gene names: 116516843

Gene position: 1763735-1762452 (Counterclockwise)

Preceding gene: 116516010

Following gene: 116516278

Centisome position: 86.2

GC content: 46.81

Gene sequence:

>1284_bases
ATGGATAAAATTGTGGTTCAAGGTGGCGATAATCGTCTGGTAGGAAGCGTGACGATCGAGGGAGCAAAAAATGCAGTCTT
ACCCTTGTTGGCAGCGACTATTCTAGCAAGTGAAGGAAAGACCGTCTTGCAGAATGTTCCGATTTTGTCGGATGTCTTTA
TTATGAATCAGGTAGTTGGTGGTTTGAATGCCAAGGTTGACTTTGATGAGGAAGCTCATCTTGTCAAGGTGGATGCTACT
GGCGACATCACTGAGGAAGCCCCTTACAAGTATGTCAGCAAGATGCGCGCCTCCATCGTTGTATTAGGGCCAATCCTTGC
CCGTGTGGGTCATGCCAAGGTATCCATGCCAGGTGGTTGTACGATTGGTAGCCGTCCTATTGATCTTCATTTGAAAGGTC
TGGAAGCTATGGGGGTTAAGATTAGTCAGACAGCTGGTTACATCGAAGCCAAGGCAGAACGCTTGCATGGCGCTCATATC
TATATGGACTTTCCAAGTGTTGGTGCAACGCAGAACTTGATGATGGCAGCGACTCTGGCTGATGGGGTGACAGTGATTGA
GAATGCTGCGCGTGAGCCTGAGATTGTTGACTTAGCCATTCTCCTTAATGAAATGGGAGCCAAGGTCAAAGGTGCTGGTA
CAGAGACTATAACCATTACTGGTGTTGAGAAACTTCATGGTACGACTCACAATGTAGTCCAAGACCGTATCGAAGCAGGA
ACCTTTATGGTAGCTGCTGCCATGACTGGTGGTGATGTCTTGATTCGAGACGCTGTCTGGGAGCACAACCGTCCCTTGAT
TGCCAAGTTACTTGAAATGGGTGTTGAAGTAATTGAAGAAGACGAAGGAATTCGTGTTCGTTCTCAACTAGAAAATCTAA
AAGCTGTTCATGTGAAAACCTTGCCCCACCCAGGATTTCCAACAGATATGCAGGCTCAATTTACAGCCTTGATGACAGTT
GCAAAAGGCGAATCAACCATGGTGGAGACAGTTTTCGAAAATCGTTTCCAACACCTAGAAGAGATGCGCCGCATGGGCTT
GCATTCTGAGATTATCCGTGATACAGCTCGTATTGTTGGTGGACAGCCTTTGCAGGGAGCAGAAGTTCTTTCAACTGACC
TTCGTGCCAGTGCAGCCTTGATTTTGACAGGTTTGGTAGCACAGGGAGAAACTGTGGTCGGTAAATTGGTTCACTTGGAT
AGAGGTTACTACGGTTTCCATGAGAAGTTGGCGCAGCTAGGTGCTAAGATTCAGCGGATTGAGGCAAATGATGAAGATGA
ATAA

Upstream 100 bases:

>100_bases
TCATAGGCTACATCCTGAGTTCTTTCTTTATTAGCCTGTATCATTTGTGGCAAGAAGCGCTTAGAGGATTATTATGAAAT
CAAGAGTAAAGGAAACGAGT

Downstream 100 bases:

>100_bases
GAAATCAAGCTACGTAGTCAAGCGTTTACTTTTAGTCATCATAGTACTGATTTTAGGTACTCTGGCTCTAGGAATCGGTT
TAATGGTAGGTTATGGAATC

Product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Products: NA

Alternate protein names: Enoylpyruvate transferase 1; UDP-N-acetylglucosamine enolpyruvyl transferase 1; EPT 1 [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQNVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDAT
GDITEEAPYKYVSKMRASIVVLGPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITGVEKLHGTTHNVVQDRIEAG
TFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTV
AKGESTMVETVFENRFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETVVGKLVHLD
RGYYGFHEKLAQLGAKIQRIEANDEDE

Sequences:

>Translated_427_residues
MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQNVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDAT
GDITEEAPYKYVSKMRASIVVLGPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITGVEKLHGTTHNVVQDRIEAG
TFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTV
AKGESTMVETVFENRFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETVVGKLVHLD
RGYYGFHEKLAQLGAKIQRIEANDEDE
>Mature_427_residues
MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQNVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDAT
GDITEEAPYKYVSKMRASIVVLGPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITGVEKLHGTTHNVVQDRIEAG
TFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTV
AKGESTMVETVFENRFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETVVGKLVHLD
RGYYGFHEKLAQLGAKIQRIEANDEDE

Specific function: Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine [H]

COG id: COG0766

COG function: function code M; UDP-N-acetylglucosamine enolpyruvyl transferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family. MurA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789580, Length=422, Percent_Identity=50.4739336492891, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR013792
- InterPro:   IPR005750 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.7 [H]

Molecular weight: Translated: 45896; Mature: 45896

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQNVPILSDVFIMNQVVG
CCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHC
GLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVLGPILARVGHAKVSMPGGC
CCCCEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHEEEEHHHHHHCCCCEEECCCCC
TIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHIYMDFPSVGATQNLMMAATLA
CCCCCCEEEEEECCHHHCEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCHHEEEHHHH
DGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITGVEKLHGTTHNVVQDRIEAG
CCHHHHHHCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEEHHHHCCCHHHHHHHHHHCC
TFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIEEDEGIRVRSQLENLKAVHVKT
EEEEEEEECCCCEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEE
LPHPGFPTDMQAQFTALMTVAKGESTMVETVFENRFQHLEEMRRMGLHSEIIRDTARIVG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GQPLQGAEVLSTDLRASAALILTGLVAQGETVVGKLVHLDRGYYGFHEKLAQLGAKIQRI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHEEEE
EANDEDE
CCCCCCC
>Mature Secondary Structure
MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQNVPILSDVFIMNQVVG
CCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHC
GLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVLGPILARVGHAKVSMPGGC
CCCCEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHEEEEHHHHHHCCCCEEECCCCC
TIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHIYMDFPSVGATQNLMMAATLA
CCCCCCEEEEEECCHHHCEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCHHEEEHHHH
DGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITGVEKLHGTTHNVVQDRIEAG
CCHHHHHHCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEEHHHHCCCHHHHHHHHHHCC
TFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIEEDEGIRVRSQLENLKAVHVKT
EEEEEEEECCCCEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEE
LPHPGFPTDMQAQFTALMTVAKGESTMVETVFENRFQHLEEMRRMGLHSEIIRDTARIVG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GQPLQGAEVLSTDLRASAALILTGLVAQGETVVGKLVHLDRGYYGFHEKLAQLGAKIQRI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHEEEE
EANDEDE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]