Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 116516807

GI number: 116516807

Start: 1099803

End: 1100918

Strand: Reverse

Name: mdeA [H]

Synonym: SPD_1073

Alternate gene names: 116516807

Gene position: 1100918-1099803 (Counterclockwise)

Preceding gene: 116516884

Following gene: 116515946

Centisome position: 53.81

GC content: 42.47

Gene sequence:

>1116_bases
TTGAGGAAACCAGGGAACATTTATACTCGTATCACCAATCCTACAACAGCTGCCCTTGAAGGTGGTGTTGAAGCGCTAGC
AACAGCATCAGGTATGACTGCAGTGACTTATACGATTTTGGCGCTTGCCCATGCTGGTGAGCATGTAGTGGCTGCTTCGA
CTATTTACGGTGGAACCTTCAATCTTTTGAAAGAACCCCTTCCTCGTTATGGTATCACAACAACCTTTGTCGATGTTGAT
AATTTGGAGGAAGTAGAAGCAGCTATCAAAGACAATACCAAGCTTGTCTTGATTGAAACCTTGGGTAACCCCTTGATTAA
TATTCCAGACCTGGAAAAACTGGCAGAGATTGCTCATAAACATCAAATCCCACTTGTGTCAGACAATACTTTTGCAACAC
CTTATTTGATTAACGTCTTCTCTCATGGCGTTGACATTGCCATTCACTCTGCGACTAAGTTTATCGGTGGGCATGGTACA
ACTATTGGAGGAATAATTGTCGATAGTGGTCGTTTTGACTGGACGGCTTCAGGGAAATTCCCTCAATTTGTTGACGAGAG
TCCAAGCTGCCACAATTTGAGCTATACTCATGATGTGGGTGCAGCAGCCTTTATTATAGCTGTTCGAGTTCAATTGCTTC
GTGATACAGGTGCAGCCTTGTCACCATTCAATGCTTTCCTCTTGCTACAAAGACTTGAAACCCTTTCACTTCGTGTGGAA
CGCCATGTACAAAATGCTGAGACAATTGTTGATTTTCTTGTCAACCATCCTAAGGTAGAAAAGGTAAATTATCCAAAACT
TGCAGATAGTCCTTATTATGCCTTGGCTGAGAAATACTTGCCAAAAGGTGTCGGTTCAATCTTTACCTTCCACGTCAAAG
GTGACGAGGAGGAAGCACGCAAGGTCATTGATAATTTAGAAATCTTTTCTGACCTTGCAAACGCGGCAGATGCTAAATCG
CTTGTTGTCCATCCAGCAACAATCACTCACGGTCAATTGTCAGAAAAAGACCTAGAAGCAGCAGGTGTCACACCAAATCA
AATCCACTTGTCTATCGGTCTTGAAAATGTAGAAGATTTGATTGAAGACTTACGCTTGGCCTTGGAAAAAATTTAA

Upstream 100 bases:

>100_bases
TCAAGTTGTGGCTCCAGCTACTAAGTCTCGTGCAGTGCCGATTTATCAAACAACATTTTTTGTTTTTGATGACACGTAGG
AAGGTGCCGATCTGTTTGCC

Downstream 100 bases:

>100_bases
AGTAAAAGAAGATAAACAGTGGGTTTTGACTCACTGTTTTTGATTTTCCCTCAGGCATGATATAATGGTTACAGAAGTCT
AGAAAGAGGAATGTAATGAA

Product: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGEHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVD
NLEEVEAAIKDNTKLVLIETLGNPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGT
TIGGIIVDSGRFDWTASGKFPQFVDESPSCHNLSYTHDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETLSLRVE
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALAEKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKS
LVVHPATITHGQLSEKDLEAAGVTPNQIHLSIGLENVEDLIEDLRLALEKI

Sequences:

>Translated_371_residues
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGEHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVD
NLEEVEAAIKDNTKLVLIETLGNPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGT
TIGGIIVDSGRFDWTASGKFPQFVDESPSCHNLSYTHDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETLSLRVE
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALAEKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKS
LVVHPATITHGQLSEKDLEAAGVTPNQIHLSIGLENVEDLIEDLRLALEKI
>Mature_371_residues
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGEHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVD
NLEEVEAAIKDNTKLVLIETLGNPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGT
TIGGIIVDSGRFDWTASGKFPQFVDESPSCHNLSYTHDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETLSLRVE
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALAEKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKS
LVVHPATITHGQLSEKDLEAAGVTPNQIHLSIGLENVEDLIEDLRLALEKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=355, Percent_Identity=33.5211267605634, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI299473759, Length=348, Percent_Identity=30.7471264367816, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI25453487, Length=354, Percent_Identity=27.683615819209, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1790375, Length=364, Percent_Identity=31.043956043956, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789383, Length=363, Percent_Identity=25.3443526170799, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=376, Percent_Identity=34.0425531914894, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=358, Percent_Identity=31.8435754189944, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=361, Percent_Identity=30.4709141274238, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=387, Percent_Identity=45.4780361757106, Blast_Score=295, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6319307, Length=376, Percent_Identity=33.2446808510638, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=25.1552795031056, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321254, Length=392, Percent_Identity=23.7244897959184, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=384, Percent_Identity=24.7395833333333, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922554, Length=375, Percent_Identity=32.2666666666667, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 40246; Mature: 40246

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGEHVVAASTIYGGTF
CCCCCCEEEEECCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
NLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLGNPLINIPDLEKLAEIAHK
HHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
HQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGTTIGGIIVDSGRFDWTASGKF
CCCCEECCCCCCCHHHHHHHHCCCEEEEECHHHHHCCCCCEEEEEEEECCCCEEEECCCC
PQFVDESPSCHNLSYTHDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETLSLRVE
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALAEKYLPKGVGSIFTFHVKGDEEEAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCHHHHH
KVIDNLEIFSDLANAADAKSLVVHPATITHGQLSEKDLEAAGVTPNQIHLSIGLENVEDL
HHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCHHHHHCCCCCCEEEEEECHHHHHHH
IEDLRLALEKI
HHHHHHHHHCC
>Mature Secondary Structure
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGEHVVAASTIYGGTF
CCCCCCEEEEECCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
NLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLGNPLINIPDLEKLAEIAHK
HHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
HQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGTTIGGIIVDSGRFDWTASGKF
CCCCEECCCCCCCHHHHHHHHCCCEEEEECHHHHHCCCCCEEEEEEEECCCCEEEECCCC
PQFVDESPSCHNLSYTHDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETLSLRVE
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALAEKYLPKGVGSIFTFHVKGDEEEAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCHHHHH
KVIDNLEIFSDLANAADAKSLVVHPATITHGQLSEKDLEAAGVTPNQIHLSIGLENVEDL
HHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCHHHHHCCCCCCEEEEEECHHHHHHH
IEDLRLALEKI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]