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The map label for this gene is tilS

Identifier: 116516781

GI number: 116516781

Start: 10335

End: 11612

Strand: Direct

Name: tilS

Synonym: SPD_0011

Alternate gene names: 116516781

Gene position: 10335-11612 (Clockwise)

Preceding gene: 116515953

Following gene: 116516681

Centisome position: 0.51

GC content: 33.72

Gene sequence:

>1278_bases
ATGAGGGAACCAGATTTTTTAAATCATTTTCTCAAGAAGGGATATTTCAAAAAGCATGCTAAGGCGGTTCTAGCTCTTTC
TGGTGGATTAGATTCCATGTTTCTATTTAAGGTATTGTCTACTTATCAAAAAGAGTTAGAGATTGAATTGATTCTAGCTC
ATGTGAATCATAAGCAGAGAATTGAATCAGATTGGGAAGAAAAGGAATTAAGGAAGTTGGCTGCTGAAGCAGAGCTTCCT
ATTTATATCAGCAATTTTTCAGGAGAATTTTCAGAAGCGCGTGCACGAAATTTTCGTTATGATTTTTTTCAAGAGGTCAT
GAAAAAGACAGGTGCGACAGCTTTAGTCACTGCCCACCATGCTGATGATCAGGTGGAAACGATTTTTATGCGCTTGATTC
GAGGAACTCGCTTGCGCTATCTATCAGGAATTAAGGAGAAGCAAGTAGTCGGAGAGATAGAAATCATTCGTCCCTTCTTG
CATTTTCAGAAAAAAGACTTTCCATCAATTTTTCACTTTGAAGATACATCAAATCAGGAGAATCATTATTTTCGAAATCG
TATTCGAAATTCTTACTTACCAGAATTGGAAAAAGAAAATCCTCGATTTAGGGATGCAATCTTAGGCATTGGCAATGAAA
TTTTAGATTATGATTTGGCAATAGCTGAATTATCTAACAATATTAATGTGGAAGATTTACAGCAGTTATTTTCTTACTCT
GAGTCTACACAAAGAGTTTTACTTCAAACTTATCTGAATCGTTTTCCAGATTTGAATCTTACAAAAGCTCAGTTTGCTGA
AGTTCAGCAGATTTTAAAATTTAAAAGCCAGTATCGTCATCCGATTAAAAATGGCTATGAATTGATAAAAGAGTACCAAC
AGTTTCAGATTTGTAAAATCAGTCCGCAGGCTGATGAAAAGGAAGATGAACTTGTGTTACACTATCAAAATCAGGTAGCT
TATCAAGGATATTTATTTTCTTTTGGACTTCCATTAGAAGGTGAATTAATTCAACAAATACCTGTTTCACGTGAAACATC
CATACACATTCGTCATCGAAAAACAGGAGATGTTTTGATTAAAAATGGGCATAGAAAAAAACTCAGACGTTTATTTATTG
ATTTGAAAATCCCTATGGAAAAGAGAAACTCTGCTCTTATTATTGAGCAATTTGGTGAAATTGTCTCAATTTTGGGAATT
GCGACCAATAATTTGAGTAAAAAAACGAAAAATGATATAATGAACACTGTACTTTATATAGAAAAAATAGATAGGTAA

Upstream 100 bases:

>100_bases
TTGTCTATGCAGATTCTCCATTTATTCTTTCTATTTTCACTAAGAATTCTGATTATGATACGATTTCTAAGATAGCCAAG
GATGTTTATGAGGTTCTAAA

Downstream 100 bases:

>100_bases
AAAATGTTAGAAAACGATATTAAAAAAGTCCTCGTTTCACACGATGAAATTACAGAAGCAGCTAAAAAACTAGGTGCTCA
ATTAACTAAAGACTATGCAG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQRIESDWEEKELRKLAAEAELP
IYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKFKSQYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVA
YQGYLFSFGLPLEGELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGI
ATNNLSKKTKNDIMNTVLYIEKIDR

Sequences:

>Translated_425_residues
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQRIESDWEEKELRKLAAEAELP
IYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKFKSQYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVA
YQGYLFSFGLPLEGELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGI
ATNNLSKKTKNDIMNTVLYIEKIDR
>Mature_425_residues
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQRIESDWEEKELRKLAAEAELP
IYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKFKSQYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVA
YQGYLFSFGLPLEGELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGI
ATNNLSKKTKNDIMNTVLYIEKIDR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=413, Percent_Identity=23.2445520581114, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_STRP2 (Q04N53)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815434.1
- ProteinModelPortal:   Q04N53
- SMR:   Q04N53
- STRING:   Q04N53
- EnsemblBacteria:   EBSTRT00000020579
- GeneID:   4442234
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0011
- eggNOG:   COG0037
- GeneTree:   EBGT00050000027463
- HOGENOM:   HBG697356
- OMA:   GHHKKLR
- ProtClustDB:   CLSK876678
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 49967; Mature: 49967

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQR
CCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHH
IESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHH
HHCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
ADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFLHFQKKDFPSIFHFEDTSNQE
CCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
NHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKFKSQYRHPIKNGYELIKEYQQFQICKI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEE
SPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEGELIQQIPVSRETSIHIRHRKTGDVLI
CCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEE
KNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGIATNNLSKKTKNDIMNTVLYI
ECCCHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKIDR
HHCCC
>Mature Secondary Structure
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQR
CCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHH
IESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHH
HHCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
ADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFLHFQKKDFPSIFHFEDTSNQE
CCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
NHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKFKSQYRHPIKNGYELIKEYQQFQICKI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEE
SPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEGELIQQIPVSRETSIHIRHRKTGDVLI
CCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEE
KNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGIATNNLSKKTKNDIMNTVLYI
ECCCHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKIDR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA