Click here to switch to the map view.

The map label for this gene is tig

Identifier: 116516755

GI number: 116516755

Start: 367473

End: 368756

Strand: Direct

Name: tig

Synonym: SPD_0365

Alternate gene names: 116516755

Gene position: 367473-368756 (Clockwise)

Preceding gene: 116516231

Following gene: 116516919

Centisome position: 17.96

GC content: 41.04

Gene sequence:

>1284_bases
ATGTCTGTATCATTTGAAAACAAAGAAACAAACCGTGGTGTCTTGACTTTCACTATCTCTCAAGACCAAATCAAACCAGA
ATTGGACCGTGTCTTCAAGTCAGTGAAGAAATCTCTTAATGTTCCAGGTTTCCGTAAAGGTCACCTTCCACGCCCTATCT
TCGACCAAAAATTTGGTGAAGAAGCTCTTTATCAAGATGCAATGAACGCACTTTTGCCAAACGCTTATGAAGCAGCTGTA
AAAGAAGCTGGTCTTGAAGTGGTTGCCCAACCAAAAATTGACGTAACTTCAATGGAAAAAGGTCAAGACTGGGTTATCAC
TGCTGAAGTCGTTACAAAACCTGAAGTAAAATTGGGTGACTACAAAAACCTTGAAGTATCAGTTGATGTAGAAAAAGAAG
TAACTGACGCTGATGTCGAAGAGCGTATCGAACGCGAACGCAACAACCTGGCTGAATTGGTTATCAAGGAAGCTGCTGCT
GAAAACGGCGACACTGTTGTGATCGACTTCGTTGGTTCTATCGACGGTGTTGAATTTGACGGTGGAAAAGGTGAAAACTT
CTCACTTGGACTTGGTTCAGGTCAATTCATCCCTGGTTTCGAAGACCAATTGGTAGGTCACTCAGCTGGCGAAACCGTTG
ATGTTATCGTAACATTCCCAGAAGACTACCAAGCAGAAGACCTTGCAGGTAAAGAAGCTAAATTCGTGACAACTATCCAC
GAAGTAAAAGCTAAAGAAGTTCCAGCTCTTGACGATGAACTTGCAAAAGACATTGATGAAGAAGTTGAAACACTTGCTGA
CTTGAAAGAAAAATACCGCAAAGAATTGGCTGCTGCTAAAGAAGAAACTTACAAAGATGCAGTTGAAGGTGCAGCAATTG
ATACAGCTGTAGAAAACGCTGAAATCGTAGAACTTCCAGAAGAAATGATCCATGAAGAAGTTCACCGTTCAGTAAATGAA
TTCCTTGGGAACTTGCAACGTCAAGGGATCAACCCTGACATGTACTTCCAAATCACTGGAACTACTCAAGAAGACCTTCA
CAACCAATACCAAGCAGAAGCTGAGTCACGTACTAAGACTAACCTTGTTATCGAAGCAGTTGCCAAAGCTGAAGGATTTG
ATGCTTCAGAAGAAGAAATACAAAAAGAAGTTGAGCAATTGGCAGCAGACTACAACATGGAAGTTGCACAAGTTCAAAAC
TTGCTTTCAGCTGACATGTTGAAACATGATATCACTATCAAAAAAGCTGTTGAATTGATCACAAGCACAGCAACAGTAAA
ATAA

Upstream 100 bases:

>100_bases
AAAGGCTTGTTGTCTATAATTGGCTGCATTAGTCTAGATTTTATTTATAGAAAATGTTATAATAGACTGTATTTAAAAAA
TTTTAAGGAGAAATGACAGA

Downstream 100 bases:

>100_bases
TCTTAATAAACAGAAAACCCACCTGAATTGGTGGGTTTTCTGATGCACTATTTTCCAAAAATCTCTTTGAGGTCTGTGTC
TGTAATCCCAATCATGGCTG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAV
KEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAKFVTTIH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEETYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNE
FLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQN
LLSADMLKHDITIKKAVELITSTATVK

Sequences:

>Translated_427_residues
MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAV
KEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAKFVTTIH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEETYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNE
FLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQN
LLSADMLKHDITIKKAVELITSTATVK
>Mature_426_residues
SVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVK
EAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAAE
NGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAKFVTTIHE
VKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEETYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEF
LGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQNL
LSADMLKHDITIKKAVELITSTATVK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=397, Percent_Identity=31.2342569269521, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_STRP2 (Q04M73)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815875.1
- ProteinModelPortal:   Q04M73
- STRING:   Q04M73
- EnsemblBacteria:   EBSTRT00000020074
- GeneID:   4442266
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0365
- eggNOG:   COG0544
- GeneTree:   EBGT00050000027887
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47298; Mature: 47167

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDQKFGE
CCCCCCCCCCCCCEEEEEECHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCH
EALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGD
HHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
YKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAAENGDTVVIDFVGSIDGVEFD
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAKFVTTIH
CCCCCEEEEECCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCHHHHHHHH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEETYKDAVEGAAIDTAVENA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
EIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKT
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
NLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQNLLSADMLKHDITIKKAVELI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSTATVK
HHHHCCC
>Mature Secondary Structure 
SVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDQKFGE
CCCCCCCCCCCCEEEEEECHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCH
EALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGD
HHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
YKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAAENGDTVVIDFVGSIDGVEFD
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAKFVTTIH
CCCCCEEEEECCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCHHHHHHHH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEETYKDAVEGAAIDTAVENA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
EIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKT
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
NLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQNLLSADMLKHDITIKKAVELI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSTATVK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA