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The map label for this gene is lacF [H]

Identifier: 116516754

GI number: 116516754

Start: 1509645

End: 1510532

Strand: Reverse

Name: lacF [H]

Synonym: SPD_1494

Alternate gene names: 116516754

Gene position: 1510532-1509645 (Counterclockwise)

Preceding gene: 116515405

Following gene: 116516043

Centisome position: 73.82

GC content: 39.53

Gene sequence:

>888_bases
GTGAAAGTCAATAAAATCCGTATGCGGGAAACAGTGATTTCCTACGCTTTCCTAGCACCAGTATTATTCTTCTTTGTCAT
CTTTGTGTTGGCTCCGATGGTGATGGGCTTCATTACAAGTTTCTTTAACTACTCAATGACTAAATTTGAGTTTGTAGGCT
TGGATAACTATATCCGTATGTTTAAAGATCCTGTCTTTACAAAATCTCTGATTAACACAGTTATTTTGGTTATTGGATCT
GTACCAGTTGTTGTTCTATTCTCACTCTTTGTAGCATCTCAGACCTATCATCAAAATGTCATTGCCAGATCCTTCTACCG
TTTCGTCTTCTTCCTTCCTGTTGTAACGGGTAGTGTTGCCGTGACAGTTGTTTGGAAATGGATTTATGACCCACTATCAG
GGATTCTAAACTTTGTCCTTAAGTCAAGCCACATCATCAGCCAAAACATTTCTTGGTTGGGAGATAAAAACTGGGCATTG
ATGGCGATTATGATTATTCTCTTGACCACTTCAGTTGGTCAGCCCATCATCCTTTATATCGCTGCCATGGGGAATATTGA
CAATTCACTGGTTGAAGCGGCGCGTGTTGATGGTGCAACTGAGTTTCAAGTTTTCTGGAAGATTAAATGGCCAAGCCTTC
TTCCAACAACTCTTTATATTGCAATCATCACAACAATTAACTCATTCCAGTGTTTCGCCTTGATTCAGCTTTTGACATCT
GGTGGTCCAAACTACTCAACAAGTACCTTGATGTACTACCTTTACGAAAAAGCCTTCCAATTGACAGAATACGGCTATGC
CAACACAATTGGTGTCTTCTTGGCAGTCATGATTGCTATCGTAAGCTTTGTTCAATTTAAAGTACTTGGAAACGACGTAG
AATACTAA

Upstream 100 bases:

>100_bases
TGAAGTTCTTACATAAGCGAATCATAAAAAATTTCATTTTGATTTTAAAACAGTTCAAGAAAGTCAAAAAATTATTCTAT
TTGAAAGAGAGGTGCCGACT

Downstream 100 bases:

>100_bases
AGAAAGGAGACAGCTATGCAATCTACAGAAAAAAAACCATTAACAGCCTTTACTGTTATTTCAACAATCATTTTGCTCTT
GTTGACTGTGCTGTTCATCT

Product: sugar ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKVNKIRMRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEFVGLDNYIRMFKDPVFTKSLINTVILVIGS
VPVVVLFSLFVASQTYHQNVIARSFYRFVFFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWAL
MAIMIILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITTINSFQCFALIQLLTS
GGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSFVQFKVLGNDVEY

Sequences:

>Translated_295_residues
MKVNKIRMRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEFVGLDNYIRMFKDPVFTKSLINTVILVIGS
VPVVVLFSLFVASQTYHQNVIARSFYRFVFFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWAL
MAIMIILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITTINSFQCFALIQLLTS
GGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSFVQFKVLGNDVEY
>Mature_295_residues
MKVNKIRMRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEFVGLDNYIRMFKDPVFTKSLINTVILVIGS
VPVVVLFSLFVASQTYHQNVIARSFYRFVFFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWAL
MAIMIILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITTINSFQCFALIQLLTS
GGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSFVQFKVLGNDVEY

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=301, Percent_Identity=25.2491694352159, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787570, Length=255, Percent_Identity=28.2352941176471, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790465, Length=254, Percent_Identity=26.7716535433071, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33418; Mature: 33418

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNKIRMRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEFVGLDNYIRM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECHHHHHHH
FKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFVFFLPVVTGSVA
HHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIILLTTSVGQPIILYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHE
AAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITTINSFQCFALIQLLTS
EHHCCCCHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSFVQFKVLGNDVEY
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKVNKIRMRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEFVGLDNYIRM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECHHHHHHH
FKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFVFFLPVVTGSVA
HHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIILLTTSVGQPIILYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHE
AAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITTINSFQCFALIQLLTS
EHHCCCCHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSFVQFKVLGNDVEY
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]