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The map label for this gene is groEL [H]

Identifier: 116516636

GI number: 116516636

Start: 1709934

End: 1711556

Strand: Reverse

Name: groEL [H]

Synonym: SPD_1709

Alternate gene names: 116516636

Gene position: 1711556-1709934 (Counterclockwise)

Preceding gene: 116516929

Following gene: 116515970

Centisome position: 83.65

GC content: 44.42

Gene sequence:

>1623_bases
ATGTCAAAAGAAATTAAATTTTCATCAGATGCCCGTTCAGCCATGGTTCGTGGTGTCGATATCCTTGCAGACACTGTTAA
AGTAACCTTGGGACCAAAAGGTCGCAATGTCGTTCTTGAAAAGTCATTCGGTTCACCCTTGATTACCAATGACGGTGTGA
CCATTGCCAAAGAAATCGAATTGGAAGACCATTTTGAAAATATGGGTGCTAAGTTAGTATCAGAAGTAGCTTCTAAAACC
AATGATATCGCAGGTGACGGGACTACGACTGCAACAGTCTTGACCCAAGCTATCGTCCGTGAAGGAATCAAAAACGTCAC
AGCAGGTGCAAATCCAATCGGTATTCGTCGTGGGATTGAAACAGCAGTTGCCGCAGCAGTTGAAGCTTTGAAAAACAACG
CCATCCCTGTTGCCAATAAAGAAGCTATCGCTCAAGTTGCAGCCGTATCTTCTCGTTCTGAAAAAGTTGGTGAGTACATC
TCTGAAGCAATGGAAAAAGTTGGCAAAGACGGTGTCATCACCATCGAAGAGTCACGTGGTATGGAAACAGAGCTTGAAGT
CGTAGAAGGAATGCAGTTTGACCGTGGTTACCTTTCACAGTACATGGTGACTGATAGCGAAAAAATGGTGGCTGACCTTG
AAAATCCGTACATTTTGATTACAGACAAGAAAATTTCCAATATCCAAGAAATCTTGCCACTTTTGGAAAGCATTCTCCAA
AGCAATCGTCCACTCTTGATTATTGCGGATGATGTGGATGGCGAGGCTCTTCCAACTCTTGTTTTGAACAAGATTCGTGG
AACCTTCAACGTAGTAGCAGTCAAGGCACCTGGTTTTGGTGACCGTCGCAAAGCCATGCTTGAAGATATCGCCATCTTAA
CAGGCGGAACAGTTATCACAGAAGACCTTGGTCTTGAGTTGAAAGATGCGACAATTGAAGCTCTTGGTCAAGCAGCGAGA
GTGACCGTGGACAAAGATAGCACGGTTATTGTAGAAGGTGCAGGAAATCCTGAAGCGATTTCTCACCGTGTTGCGGTTAT
CAAGTCTCAAATCGAAACTACAACTTCTGAATTTGACCGTGAAAAATTGCAAGAACGCTTGGCCAAATTGTCAGGTGGTG
TAGCGGTTATTAAGGTTGGAGCCGCAACTGAAACTGAGTTGAAAGAAATGAAACTCCGCATTGAAGATGCCCTCAACGCT
ACTCGTGCAGCTGTTGAAGAAGGTATTGTTGCAGGTGGTGGAACAGCTCTTGCCAATGTGATTCCAGCTGTTGCTACCTT
GGAATTGACAGGAGATGAAGCAACAGGACGTAATATTGTTCTCCGTGCTTTGGAAGAACCCGTTCGTCAAATTGCTCACA
ATGCAGGATTTGAAGGATCTATCGTTATCGATCGTTTGAAAAATGCTGAGCTTGGTATAGGATTTAACGCAGCAACTGGC
GAGTGGGTTAACATGATTGATCAAGGTATCATTGATCCAGTTAAAGTGAGTCGTTCAGCCCTACAAAATGCAGCATCTGT
AGCCAGCTTGATTTTGACAACAGAAGCAGTCGTAGCCAATAAACCAGAACCAGTAGCCCCAGCTCCAGCAATGGATCCAA
GCATGATGGGCGGGATGATGTAA

Upstream 100 bases:

>100_bases
CCACGCAGGTCTTGATGTCAAAGATGGCGATGAAAAGTACATCATCGTAGGCGAAGCTAACATTTTGGCAATCATTGAGG
AATAGAAGGAGAAAGTAAGT

Downstream 100 bases:

>100_bases
GCTTTCTATAGAAAACAACTTATAAAAAACACAAAAGGAGGGAATGACTAACCCTTCTTTTTATAGGCTCTTTGTCAACT
GTAGTGGGTTGAAGTCAGCT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFGSPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKT
NDIAGDGTTTATVLTQAIVREGIKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYILITDKKISNIQEILPLLESILQ
SNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAAR
VTVDKDSTVIVEGAGNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKLRIEDALNA
TRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQIAHNAGFEGSIVIDRLKNAELGIGFNAATG
EWVNMIDQGIIDPVKVSRSALQNAASVASLILTTEAVVANKPEPVAPAPAMDPSMMGGMM

Sequences:

>Translated_540_residues
MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFGSPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKT
NDIAGDGTTTATVLTQAIVREGIKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYILITDKKISNIQEILPLLESILQ
SNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAAR
VTVDKDSTVIVEGAGNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKLRIEDALNA
TRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQIAHNAGFEGSIVIDRLKNAELGIGFNAATG
EWVNMIDQGIIDPVKVSRSALQNAASVASLILTTEAVVANKPEPVAPAPAMDPSMMGGMM
>Mature_539_residues
SKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFGSPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTN
DIAGDGTTTATVLTQAIVREGIKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYIS
EAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYILITDKKISNIQEILPLLESILQS
NRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARV
TVDKDSTVIVEGAGNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKLRIEDALNAT
RAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQIAHNAGFEGSIVIDRLKNAELGIGFNAATGE
WVNMIDQGIIDPVKVSRSALQNAASVASLILTTEAVVANKPEPVAPAPAMDPSMMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=526, Percent_Identity=50.7604562737643, Blast_Score=521, Evalue=1e-148,
Organism=Homo sapiens, GI31542947, Length=526, Percent_Identity=50.7604562737643, Blast_Score=521, Evalue=1e-148,
Organism=Homo sapiens, GI302058290, Length=518, Percent_Identity=21.8146718146718, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI58331173, Length=550, Percent_Identity=21.4545454545455, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI5453603, Length=559, Percent_Identity=23.613595706619, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI5453607, Length=560, Percent_Identity=22.8571428571429, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI24307939, Length=361, Percent_Identity=25.207756232687, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790586, Length=524, Percent_Identity=61.8320610687023, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=527, Percent_Identity=48.9563567362429, Blast_Score=490, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI193210679, Length=206, Percent_Identity=48.0582524271845, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25144674, Length=173, Percent_Identity=31.7919075144509, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=53.8022813688213, Blast_Score=531, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6320058, Length=186, Percent_Identity=26.3440860215054, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322524, Length=142, Percent_Identity=29.5774647887324, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641193, Length=526, Percent_Identity=50.3802281368821, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24641191, Length=526, Percent_Identity=50.3802281368821, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=47.1374045801527, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=47.1374045801527, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=47.1374045801527, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864606, Length=523, Percent_Identity=43.4034416826004, Blast_Score=433, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24584129, Length=535, Percent_Identity=36.6355140186916, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI19921262, Length=535, Percent_Identity=36.6355140186916, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI17647245, Length=459, Percent_Identity=24.400871459695, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24652903, Length=446, Percent_Identity=24.6636771300448, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645179, Length=511, Percent_Identity=22.5048923679061, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583944, Length=181, Percent_Identity=26.5193370165746, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57096; Mature: 56965

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFGSPLITNDGVTIAKEIE
CCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCEEECCCCEEEEECC
LEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREGIKNVTAGANPIGIRRGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYISEAMEKVGKDGVITIEESRG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
METELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYILITDKKISNIQEILPLLESILQ
CCHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHH
SNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGTVIT
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEE
EDLGLELKDATIEALGQAARVTVDKDSTVIVEGAGNPEAISHRVAVIKSQIETTTSEFDR
CCCCCEEHHHHHHHHCCHHEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLSGGVAVIKVGAATETELKEMKLRIEDALNATRAAVEEGIVAGGGTALANV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IPAVATLELTGDEATGRNIVLRALEEPVRQIAHNAGFEGSIVIDRLKNAELGIGFNAATG
HHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCC
EWVNMIDQGIIDPVKVSRSALQNAASVASLILTTEAVVANKPEPVAPAPAMDPSMMGGMM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFGSPLITNDGVTIAKEIE
CCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCEEECCCCEEEEECC
LEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREGIKNVTAGANPIGIRRGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYISEAMEKVGKDGVITIEESRG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
METELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYILITDKKISNIQEILPLLESILQ
CCHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHH
SNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGTVIT
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEE
EDLGLELKDATIEALGQAARVTVDKDSTVIVEGAGNPEAISHRVAVIKSQIETTTSEFDR
CCCCCEEHHHHHHHHCCHHEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLSGGVAVIKVGAATETELKEMKLRIEDALNATRAAVEEGIVAGGGTALANV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IPAVATLELTGDEATGRNIVLRALEEPVRQIAHNAGFEGSIVIDRLKNAELGIGFNAATG
HHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCC
EWVNMIDQGIIDPVKVSRSALQNAASVASLILTTEAVVANKPEPVAPAPAMDPSMMGGMM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12202549 [H]