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The map label for this gene is prsA

Identifier: 116516609

GI number: 116516609

Start: 28634

End: 29602

Strand: Direct

Name: prsA

Synonym: SPD_0033

Alternate gene names: 116516609

Gene position: 28634-29602 (Clockwise)

Preceding gene: 116515789

Following gene: 116516654

Centisome position: 1.4

GC content: 41.9

Gene sequence:

>969_bases
ATGTCTTTTTCTGATTTAAAGCTGTTTGCCCTTTCTTCTAATAAAGAATTGGCAGAACGTGTGGCGCAGGAGATTGGGAT
AGAGTTGGGGAAATCAAGTGTTCGCCAATTTTCAGATGGAGAGATTCAGGTCAACATTGAAGAATCAATCCGTGGGAAAC
ACGTCTTTATCTTACAATCAACTAGTTCGCCTGTAAATGACAATCTGCTTGAAATTTTGATTATGGTAGATGCTTTGAAG
CGTGCGAGTGCAGAGTCTGTCAATGTTGTCATGCCTTACTATGGGTATGCACGTCAGGATAGAAAGGCGAGAGCGCGTGA
GCCAATCACTTCAAAACTTGTCGCAAATATGCTTGAAGTAGCTGGAGTGGATCGTTTATTGACCATCGACTTGCATGCTG
CGCAAATTCAAGGATTCTTTGATATTCCTGTGGATCATTTGATGGGTGCTCCTCTAATTGCAGATTATTTTGAGCGGCGT
GGTATGGTTGGTTCTGACTATGTGGTTGTCAGCCCGGACCATGGAGGGGTGACTCGTGCTCGTAAGTTGGCAGAATTTTT
GAAAACATCTATCGCTATTATTGATAAACGTCGTAGCGTTGATAAGATGAATACTAGTGAAGTTATGAACATCATCGGTA
AGGTTGAAGGCAAGACTTGTATCTTGATTGATGATATGATTGATACCGCTGGAACGATTTGTCATGCGGCAGATGCTCTT
GCGGAAGCTGGTGCTGTTGAAGTCTATGCAAGCTGTACGCACCCAGTTCTTTCTGGTCCTGCTACGGACAATATCCAAAA
ATCAGCTATTAAGAAATTGGTTGTTTTGGATACCATCTATCTGCCAGAAGAGCGTTTGATTGATAAGATTGAGCAGATTT
CAATCGCTCATCTCCTAGGGGATGCTATCGTACGTATTCATGAAAAACGACCACTTTCTCCACTTTTCGATATTGAGAAA
AAAATTTAA

Upstream 100 bases:

>100_bases
TTCTTTTATTTTTGATTGAAAAATAGTGCAAAAAGCGCTACAATGGTAGATGGAAAATCTTGTGAAAAGCACAAGCGATA
CATATATACCGGAGGAAATC

Downstream 100 bases:

>100_bases
TGACCAAGCCTGAGATGATTCTCAGGTTTTTCGTCTTTTTTCCGAATAAATAGATAGTAGCGGTGAATCTAGTAAAGCTA
GATTTAAAACTGTGATATAA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 1; Phosphoribosyl pyrophosphate synthase 1; P-Rib-PP synthase 1; PRPP synthase 1

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQVNIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALK
RASAESVNVVMPYYGYARQDRKARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGKTCILIDDMIDTAGTICHAADAL
AEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVLDTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEK
KI

Sequences:

>Translated_322_residues
MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQVNIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALK
RASAESVNVVMPYYGYARQDRKARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGKTCILIDDMIDTAGTICHAADAL
AEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVLDTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEK
KI
>Mature_321_residues
SFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQVNIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKR
ASAESVNVVMPYYGYARQDRKARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERRG
MVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGKTCILIDDMIDTAGTICHAADALA
EAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVLDTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKK
I

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=315, Percent_Identity=46.031746031746, Blast_Score=280, Evalue=8e-76,
Organism=Homo sapiens, GI4506127, Length=315, Percent_Identity=45.3968253968254, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI84875539, Length=318, Percent_Identity=45.5974842767296, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=43.7699680511182, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI4506133, Length=344, Percent_Identity=36.9186046511628, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=33.9130434782609, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI310128524, Length=142, Percent_Identity=34.5070422535211, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI310115209, Length=142, Percent_Identity=34.5070422535211, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI310118259, Length=142, Percent_Identity=34.5070422535211, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI310119946, Length=142, Percent_Identity=34.5070422535211, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787458, Length=316, Percent_Identity=50, Blast_Score=320, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=46.0063897763578, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=45.4258675078864, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=45.4258675078864, Blast_Score=277, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=46.1290322580645, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=31.858407079646, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320946, Length=318, Percent_Identity=42.4528301886792, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319403, Length=318, Percent_Identity=41.1949685534591, Blast_Score=245, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6321776, Length=318, Percent_Identity=41.5094339622642, Blast_Score=238, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=40.1015228426396, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=86, Percent_Identity=46.5116279069767, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=45.0793650793651, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=42.3076923076923, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24651458, Length=357, Percent_Identity=34.1736694677871, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24651456, Length=357, Percent_Identity=34.1736694677871, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI281362873, Length=357, Percent_Identity=34.1736694677871, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24651454, Length=357, Percent_Identity=34.1736694677871, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24651462, Length=376, Percent_Identity=32.1808510638298, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24651464, Length=376, Percent_Identity=32.1808510638298, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45552010, Length=376, Percent_Identity=32.1808510638298, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS1_STRPN (P65239)

Other databases:

- EMBL:   AE005672
- PIR:   A95003
- RefSeq:   NP_344578.1
- ProteinModelPortal:   P65239
- SMR:   P65239
- EnsemblBacteria:   EBSTRT00000026757
- GeneID:   929774
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0027
- TIGR:   SP_0027
- GeneTree:   EBGT00050000027352
- HOGENOM:   HBG519284
- OMA:   CATHAVF
- ProtClustDB:   PRK02269
- BioCyc:   SPNE170187-1:SP_0027-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 35451; Mature: 35320

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQVNIEESIRGKHVFILQS
CCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEHHCCCCCEEEEEEC
TSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRKARAREPITSKLVANMLEV
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
AGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERRGMVGSDYVVVSPDHGGVTRA
HCCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
RKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGKTCILIDDMIDTAGTICHAADAL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHH
AEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVLDTIYLPEERLIDKIEQISIAHLLG
HHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DAIVRIHEKRPLSPLFDIEKKI
HHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQVNIEESIRGKHVFILQS
CCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEHHCCCCCEEEEEEC
TSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRKARAREPITSKLVANMLEV
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
AGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERRGMVGSDYVVVSPDHGGVTRA
HCCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
RKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGKTCILIDDMIDTAGTICHAADAL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHH
AEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVLDTIYLPEERLIDKIEQISIAHLLG
HHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DAIVRIHEKRPLSPLFDIEKKI
HHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916