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The map label for this gene is celD [H]

Identifier: 116516546

GI number: 116516546

Start: 279746

End: 281092

Strand: Direct

Name: celD [H]

Synonym: SPD_0283

Alternate gene names: 116516546

Gene position: 279746-281092 (Clockwise)

Preceding gene: 116515501

Following gene: 116515742

Centisome position: 13.67

GC content: 37.94

Gene sequence:

>1347_bases
ATGGATCAACAAAACGGGTTGTTTGGTTTCCTTGAAAACCATGTTATGGAACCAATGGGCAAACTTGCCCAGTTTAAAGT
AGTACGTGCTATCACGGCTGCTGGTATGGCAGCTGTACCATTTACTATTGTAGGATCAATGTTTTTGGTATTCAGTATTT
TGCCACAAGCTTTCTCATTTTGGCCAATTGTGGCAGATATTTTCTCTGCTTCATTTGGTAAATTCACATCACTTTACATG
GTTGCAAATTATGCGACTATGGGTTCTCTATCTCTTTATTTTGTTCTTTCACTTGCATATGAATTGACAAAAATTTATGC
AGAGGAAGAAGAACTCAATATGAATCCTCTTAATGGTTCCTTGCTTGCCTTGATGGCTTTTGTCATGACAGTACCGCAAA
TCATTTTTGATGGTGGAATGATGAAGGCTGTGACAAGTCTAAAAGAAGGCGCAGTAATTGCAGATGGATGGGCAATGGGA
AATGGAGTCGCACGTTTTGGGACAACAGGGATTTTTACCGCAATCATTATGGCAATTGTGACTGTTCTTATTTATCGTAT
GTGTGTTAAAAATAATTGGGTTATTAAAATGCCTGAAGCTGTTCCAGAAGGAGTTTCTCGTGGATTTACCGCTTTGATTC
CAGGATTTGTTGTTGCATTCGTTGTTATCTTTATCAACGGTCTTCTTGTAGCAATGGGAACAGATATTTTTAAAGTCATT
GCAATTCCATTTGGTTTTGTATCCAATCTGACTAATTCATGGATTGGTTTAATGATTATTTATCTGTTGACTCAACTACT
TTGGATTGTAGGTATCCACGGTGCGAACATTGTTTTTGCATTTGTTAGTCCAATTGCTCTTGCTAACATGGCTGAAAATG
CTGCTGGCGGGCACTTCGCTGTTGCAGGTGAATTTTCTAATATGTTTGTAATTGCAGGTGGTTCTGGTGCAACATTAGGC
CTATGTTTATATATTGCTTTTGCCTCTAAATCTGAACAGCTTAAAGCAATAGGACGAGCATCTGTAGTTCCAGCCTTATT
TAATATTAATGAACCATTAATTTTTGGATTACCTATTATCTATAATCCAGCCTTGGCTATACCATTTATTTTAGCACCAA
TGGTTACTGCTACTATTTATTACGTAGCGAATTCTCTAAACTTTATTAAGCCAATTATCGCACAGGTTCCATGGCCAACT
CCAGTAGGGATTGGAGCTTTCTTAGGGACAGCAGATCTTCGAGCTGTATTAGTTGCTCTCGTTTGTGCATTTGCAGCATT
CTTAGTTTATCTCCCATTCATCCGTGTATATGATCAAAAACTAGTGAAAGAAGAGCAAGGTATCTAA

Upstream 100 bases:

>100_bases
AAGAACTCACTTCGTTTATAATCCTGATCTAGTATCATTTGTATTTGAAACTAGTCTTCAAAAAGTAAAAATTATTATGT
TTTATTAAGGAGGTCTATTT

Downstream 100 bases:

>100_bases
TAAGTAAAAAAGTATACTCGATGATAGAATCCATATAGTTTAAATAATGAGTGTTAACATTAGCAGTGAAGAGATCTTTT
TAAGATATTCTTCTCTGCTT

Product: cellobiose phosphotransferase system IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MDQQNGLFGFLENHVMEPMGKLAQFKVVRAITAAGMAAVPFTIVGSMFLVFSILPQAFSFWPIVADIFSASFGKFTSLYM
VANYATMGSLSLYFVLSLAYELTKIYAEEEELNMNPLNGSLLALMAFVMTVPQIIFDGGMMKAVTSLKEGAVIADGWAMG
NGVARFGTTGIFTAIIMAIVTVLIYRMCVKNNWVIKMPEAVPEGVSRGFTALIPGFVVAFVVIFINGLLVAMGTDIFKVI
AIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGANIVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLG
LCLYIAFASKSEQLKAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPIIAQVPWPT
PVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI

Sequences:

>Translated_448_residues
MDQQNGLFGFLENHVMEPMGKLAQFKVVRAITAAGMAAVPFTIVGSMFLVFSILPQAFSFWPIVADIFSASFGKFTSLYM
VANYATMGSLSLYFVLSLAYELTKIYAEEEELNMNPLNGSLLALMAFVMTVPQIIFDGGMMKAVTSLKEGAVIADGWAMG
NGVARFGTTGIFTAIIMAIVTVLIYRMCVKNNWVIKMPEAVPEGVSRGFTALIPGFVVAFVVIFINGLLVAMGTDIFKVI
AIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGANIVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLG
LCLYIAFASKSEQLKAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPIIAQVPWPT
PVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI
>Mature_448_residues
MDQQNGLFGFLENHVMEPMGKLAQFKVVRAITAAGMAAVPFTIVGSMFLVFSILPQAFSFWPIVADIFSASFGKFTSLYM
VANYATMGSLSLYFVLSLAYELTKIYAEEEELNMNPLNGSLLALMAFVMTVPQIIFDGGMMKAVTSLKEGAVIADGWAMG
NGVARFGTTGIFTAIIMAIVTVLIYRMCVKNNWVIKMPEAVPEGVSRGFTALIPGFVVAFVVIFINGLLVAMGTDIFKVI
AIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGANIVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLG
LCLYIAFASKSEQLKAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPIIAQVPWPT
PVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=448, Percent_Identity=30.8035714285714, Blast_Score=165, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 48184; Mature: 48184

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]