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The map label for this gene is lcfB [H]

Identifier: 116516531

GI number: 116516531

Start: 955179

End: 956489

Strand: Direct

Name: lcfB [H]

Synonym: SPD_0945

Alternate gene names: 116516531

Gene position: 955179-956489 (Clockwise)

Preceding gene: 116515471

Following gene: 116515709

Centisome position: 46.68

GC content: 29.37

Gene sequence:

>1311_bases
ATGGAATTGTCGGAAATGATTAATAATGTGTTTGAAAGTTGTATACATCCTGAATATCATTTTATTGATTTATCAAAGAT
AAAAGAGAATAGGATAGCAATTATAGGAGGAAATAAATCTGAGTATTTAAATTTAATCTTAAATCTTATGCTACAAGGTA
AGGTTGTTATCCCTATTAATCCGATCCAAACTAATTCTCAAATAGAAACACTTTTGAATTTGTCTGATGTTGAAGCAGTA
TTAACAGATAATAGCTTTCAATGTAAAAGTGAGAAAAAAACGTATCTATTTTGTGAATTTAAACATATTTATAATAATCA
TGAAAGAGAGATTTCGGACACTCCTTTAGTGTTTTTTTCATCAGGTACAACAGGAGTGCCTAAGCCGGTAATGATTAATA
AATTAGCTTTTACAGAATTAATTAATCTGATGATAGCACAGTTAGAAATATCTAGTGATGATGTGTTGTATCTCCCCTCC
CACATTGCTTTCATTCAATCTCTCTGGGCTGCGATGATAGTAATTTTTTCGGGGGGAAGAGTAATTTTTAAAGATAGGAT
TACATATAAAACATTTTTTAAGGAACTTCAACTAAACGATGCAACAATAACGGTTATGGTACCTACTACGATGAATAAAC
TTGTTAATTTATATACAAATACTCTCCAAGAGTGTAAAAAATTAAGATGTATTATTGTAGGTGGAGAGATTATAAATAAG
GATAGTGCAAACTTTTTATTAAATAAATTGAAAAACACAAACATAGCAAATGCATATGGTATGGTAGAGACAACCGCAGT
TTGTTGTCTACAATATGGACGTGATTTAAAAAATATTAACTCTGTTGGTACAACTCTACCGGGGGTTGAAGTTGAAGTAA
AGAATATAGATAATGATGGTAATGGCGATATCTGGATAAAGACAAATCGCTTTATGAGCGGATATTACCCGAGTAAATAC
ACTGGTGAATGGTTATATACAGGAGATAGAGGATATAAAAATAAGGAAGGACTTTTATTTGTAACAGGCCGTGAAAGTTT
GTCAATTAATAAGGGAGGTATAAAAATTAATCCTATTGAAATTGAACAGATTATTCTTCAATTGCCAGATATTCAACTTT
GTAAAGTTGAAAAAATTAGTGATAATATTTACGGAGAAAATTATATATTAGAAGTTATTTCTGAAATTAAAGATAAAAAT
AGAATAACAGGACAGATTTTAAAAAGCTTAGGTAAATATTATTTACCTGATTCAATAAAATTTGTTGAGTCTATCGAAAC
AACTTCTAGTGGAAAATTGAAGAGAAATTAG

Upstream 100 bases:

>100_bases
TTTAGAAGAAAAGGAATAATCTTAAATTATAAAAATTATAGTCCATTTTCATTGAATTATGATTATTTAATAATTAATAA
GATTGAAGTTTAGGTGAGAT

Downstream 100 bases:

>100_bases
TATGGTTAGAAATGGAAATATTAAGTTAGATTATCCGAAAGGCTTTTTTGATCTTGATGAAATGCAATTTAAGAAGTATT
GTGACATAGAAAATCATTTA

Product: AMP-binding enzyme

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MELSEMINNVFESCIHPEYHFIDLSKIKENRIAIIGGNKSEYLNLILNLMLQGKVVIPINPIQTNSQIETLLNLSDVEAV
LTDNSFQCKSEKKTYLFCEFKHIYNNHEREISDTPLVFFSSGTTGVPKPVMINKLAFTELINLMIAQLEISSDDVLYLPS
HIAFIQSLWAAMIVIFSGGRVIFKDRITYKTFFKELQLNDATITVMVPTTMNKLVNLYTNTLQECKKLRCIIVGGEIINK
DSANFLLNKLKNTNIANAYGMVETTAVCCLQYGRDLKNINSVGTTLPGVEVEVKNIDNDGNGDIWIKTNRFMSGYYPSKY
TGEWLYTGDRGYKNKEGLLFVTGRESLSINKGGIKINPIEIEQIILQLPDIQLCKVEKISDNIYGENYILEVISEIKDKN
RITGQILKSLGKYYLPDSIKFVESIETTSSGKLKRN

Sequences:

>Translated_436_residues
MELSEMINNVFESCIHPEYHFIDLSKIKENRIAIIGGNKSEYLNLILNLMLQGKVVIPINPIQTNSQIETLLNLSDVEAV
LTDNSFQCKSEKKTYLFCEFKHIYNNHEREISDTPLVFFSSGTTGVPKPVMINKLAFTELINLMIAQLEISSDDVLYLPS
HIAFIQSLWAAMIVIFSGGRVIFKDRITYKTFFKELQLNDATITVMVPTTMNKLVNLYTNTLQECKKLRCIIVGGEIINK
DSANFLLNKLKNTNIANAYGMVETTAVCCLQYGRDLKNINSVGTTLPGVEVEVKNIDNDGNGDIWIKTNRFMSGYYPSKY
TGEWLYTGDRGYKNKEGLLFVTGRESLSINKGGIKINPIEIEQIILQLPDIQLCKVEKISDNIYGENYILEVISEIKDKN
RITGQILKSLGKYYLPDSIKFVESIETTSSGKLKRN
>Mature_436_residues
MELSEMINNVFESCIHPEYHFIDLSKIKENRIAIIGGNKSEYLNLILNLMLQGKVVIPINPIQTNSQIETLLNLSDVEAV
LTDNSFQCKSEKKTYLFCEFKHIYNNHEREISDTPLVFFSSGTTGVPKPVMINKLAFTELINLMIAQLEISSDDVLYLPS
HIAFIQSLWAAMIVIFSGGRVIFKDRITYKTFFKELQLNDATITVMVPTTMNKLVNLYTNTLQECKKLRCIIVGGEIINK
DSANFLLNKLKNTNIANAYGMVETTAVCCLQYGRDLKNINSVGTTLPGVEVEVKNIDNDGNGDIWIKTNRFMSGYYPSKY
TGEWLYTGDRGYKNKEGLLFVTGRESLSINKGGIKINPIEIEQIILQLPDIQLCKVEKISDNIYGENYILEVISEIKDKN
RITGQILKSLGKYYLPDSIKFVESIETTSSGKLKRN

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=352, Percent_Identity=26.9886363636364, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI38505220, Length=467, Percent_Identity=23.3404710920771, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI122937307, Length=360, Percent_Identity=23.6111111111111, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI156151445, Length=358, Percent_Identity=22.6256983240223, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI58082049, Length=461, Percent_Identity=20.824295010846, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157311624, Length=467, Percent_Identity=22.0556745182013, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157311622, Length=467, Percent_Identity=22.0556745182013, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693145, Length=341, Percent_Identity=24.0469208211144, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788107, Length=481, Percent_Identity=21.4137214137214, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI221142682, Length=463, Percent_Identity=22.0302375809935, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786810, Length=363, Percent_Identity=23.6914600550964, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788595, Length=285, Percent_Identity=22.4561403508772, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71996755, Length=347, Percent_Identity=26.5129682997118, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=348, Percent_Identity=25.8620689655172, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25141359, Length=426, Percent_Identity=24.6478873239437, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=341, Percent_Identity=25.2199413489736, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=350, Percent_Identity=26, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=343, Percent_Identity=22.7405247813411, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=439, Percent_Identity=20.9567198177677, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=351, Percent_Identity=23.0769230769231, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=288, Percent_Identity=24.3055555555556, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538037, Length=356, Percent_Identity=22.4719101123595, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=508, Percent_Identity=22.0472440944882, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648255, Length=346, Percent_Identity=23.9884393063584, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648253, Length=346, Percent_Identity=23.6994219653179, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356947, Length=451, Percent_Identity=21.9512195121951, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356441, Length=305, Percent_Identity=24.5901639344262, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI18859661, Length=468, Percent_Identity=23.0769230769231, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161076582, Length=291, Percent_Identity=25.7731958762887, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24653035, Length=288, Percent_Identity=25.3472222222222, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648260, Length=456, Percent_Identity=21.4912280701754, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24656500, Length=400, Percent_Identity=24.75, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648257, Length=351, Percent_Identity=23.0769230769231, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355181, Length=328, Percent_Identity=21.9512195121951, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17933690, Length=318, Percent_Identity=24.2138364779874, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281365686, Length=291, Percent_Identity=23.3676975945017, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358303, Length=295, Percent_Identity=22.7118644067797, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130357, Length=338, Percent_Identity=24.2603550295858, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922652, Length=412, Percent_Identity=21.6019417475728, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 49374; Mature: 49374

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSEMINNVFESCIHPEYHFIDLSKIKENRIAIIGGNKSEYLNLILNLMLQGKVVIPIN
CCHHHHHHHHHHHHCCCCEEEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEC
PIQTNSQIETLLNLSDVEAVLTDNSFQCKSEKKTYLFCEFKHIYNNHEREISDTPLVFFS
CCCCCHHHHHHHCHHHHHHEECCCCCEECCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEE
SGTTGVPKPVMINKLAFTELINLMIAQLEISSDDVLYLPSHIAFIQSLWAAMIVIFSGGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCE
VIFKDRITYKTFFKELQLNDATITVMVPTTMNKLVNLYTNTLQECKKLRCIIVGGEIINK
EEEECCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCEEEEECCEEECC
DSANFLLNKLKNTNIANAYGMVETTAVCCLQYGRDLKNINSVGTTLPGVEVEVKNIDNDG
CCHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCC
NGDIWIKTNRFMSGYYPSKYTGEWLYTGDRGYKNKEGLLFVTGRESLSINKGGIKINPIE
CCEEEEEECCEECCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCEEEEECCCEEECHHH
IEQIILQLPDIQLCKVEKISDNIYGENYILEVISEIKDKNRITGQILKSLGKYYLPDSIK
HHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHH
FVESIETTSSGKLKRN
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MELSEMINNVFESCIHPEYHFIDLSKIKENRIAIIGGNKSEYLNLILNLMLQGKVVIPIN
CCHHHHHHHHHHHHCCCCEEEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEC
PIQTNSQIETLLNLSDVEAVLTDNSFQCKSEKKTYLFCEFKHIYNNHEREISDTPLVFFS
CCCCCHHHHHHHCHHHHHHEECCCCCEECCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEE
SGTTGVPKPVMINKLAFTELINLMIAQLEISSDDVLYLPSHIAFIQSLWAAMIVIFSGGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCE
VIFKDRITYKTFFKELQLNDATITVMVPTTMNKLVNLYTNTLQECKKLRCIIVGGEIINK
EEEECCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCEEEEECCEEECC
DSANFLLNKLKNTNIANAYGMVETTAVCCLQYGRDLKNINSVGTTLPGVEVEVKNIDNDG
CCHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCC
NGDIWIKTNRFMSGYYPSKYTGEWLYTGDRGYKNKEGLLFVTGRESLSINKGGIKINPIE
CCEEEEEECCEECCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCEEEEECCCEEECHHH
IEQIILQLPDIQLCKVEKISDNIYGENYILEVISEIKDKNRITGQILKSLGKYYLPDSIK
HHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHH
FVESIETTSSGKLKRN
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]