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The map label for this gene is manX [H]

Identifier: 116516395

GI number: 116516395

Start: 69847

End: 70251

Strand: Direct

Name: manX [H]

Synonym: SPD_0069

Alternate gene names: 116516395

Gene position: 69847-70251 (Clockwise)

Preceding gene: 116515325

Following gene: 116515512

Centisome position: 3.41

GC content: 43.95

Gene sequence:

>405_bases
ATGACTAAATCATTAATTTTGGTGAGCCATGGTCGCTTCTGTGAGGAGCTTAGAGGTAGCACAGAAATGATTATGGGCCC
ACAAGACAACATTTACACAGTAGCTCTTCTTCCAGAAGATGGCCCAGAAGAATTTACTGCTAAATTTGAAGCTGTTATTG
AAGGGTTGGATGATTTCCTAGTCTTTGCGGATCTTCTCGGTGGGACACCTTGTAATGTGGTGAGTCGCTTGATCATGGAA
GGTCGTGATATTGACCTTTACGCAGGGATGAATCTTCCAATGGTGATTGAATTTATCAATGCGAGCCTTACAGGCGCAGA
TGCGGACTACAAGAGCCGTGCTGCAGAAAGCATTGTGAAAGTTAATGACCTGTTAGCGGGCTTCGATGATGACGAAGATG
AATAA

Upstream 100 bases:

>100_bases
TAAAAAAGGTATGAACTCTACTAAAGCTATCGGTATTATTATCGTACTTGCTTTGGCTCTTTCTGCCCTTGGTCACTTTG
CACTTGGAATGTAATTCCTT

Downstream 100 bases:

>100_bases
TACTCTTCGAAAATCTCTTCAAACTACGTCAACGTCGCCTTACCGTAGGTATATGTTACTGACTTCGTCAGTCTTATCCG
GCAACCTCAAAACGGTGTTT

Product: PTS system, IIA component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEEFTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIME
GRDIDLYAGMNLPMVIEFINASLTGADADYKSRAAESIVKVNDLLAGFDDDEDE

Sequences:

>Translated_134_residues
MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEEFTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIME
GRDIDLYAGMNLPMVIEFINASLTGADADYKSRAAESIVKVNDLLAGFDDDEDE
>Mature_133_residues
TKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEEFTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEG
RDIDLYAGMNLPMVIEFINASLTGADADYKSRAAESIVKVNDLLAGFDDDEDE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=104, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR004720 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 14670; Mature: 14539

Theoretical pI: Translated: 3.79; Mature: 3.79

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEEFTAKFEAVIEGLDDFL
CCCCEEEEECCHHHHHHCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINASLTGADADYKSRAAESIVK
HHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VNDLLAGFDDDEDE
HHHHHCCCCCCCCC
>Mature Secondary Structure 
TKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEEFTAKFEAVIEGLDDFL
CCCEEEEECCHHHHHHCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINASLTGADADYKSRAAESIVK
HHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VNDLLAGFDDDEDE
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA