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The map label for this gene is purK [H]

Identifier: 116516392

GI number: 116516392

Start: 57784

End: 58875

Strand: Direct

Name: purK [H]

Synonym: SPD_0060

Alternate gene names: 116516392

Gene position: 57784-58875 (Clockwise)

Preceding gene: 116515776

Following gene: 116516948

Centisome position: 2.82

GC content: 47.71

Gene sequence:

>1092_bases
ATGAGCTCATCTAAAACAATCGGAATTATCGGTGGCGGTCAACTGGGTCAGATGATGGCCATTTCTGCTATCTACATGGG
CCACAAGGTTATCGCGCTGGATCCTGCGGCGGATTGCCCGGCCTCTCGCGTGGCGGAAATCATTGTGGCACCTTATAACG
ATGTGGACGCCCTCCGTCAGTTGGCAGACCGTTGCGATGTCCTCACTTACGAGTTTGAAAATGTCGACGCTGACGGTTTG
GATGCCGTTATCAAGGATGGACAACTCCCTCAAGGAACAGATCTGCTCCGCATTTCGCAAAATCGTATTTTTGAAAAGGA
CTTTTTGTCAAACAAGTCTCAAGTCACTGTGGCACCCTACAAGGTCGTGACTTCTAGCCTAGACTTGGCAGATATCGACT
TGTCGAAAAACTATGTCCTCAAGACTGCGACTGGTGGCTACGATGGTCATGGACAAAAGGTTATTCGTTCAGAAGCAGAC
TTGGAAGCAGCCTATGCGCTAGCAGACTCAGCAGACTGCGTCTTGGAAGAATTTGTCAACTTTGACCTTGAGATTTCTGT
CATCGTGTCAGGAAATGGCAAGGAGGTGACGTTTTTCCCAGTTCAGGAAAATATCCACCGCAACAATATCCTGTCTAAGA
CCATCGTACCAGCCCGCATTTCTGAAAGTCTAGTAGATAAGGCTAAAGCTATGGCAGTGCGAATCGCAGAACAACTCAAC
TTGTCTGGAACTCTCTGTGTGGAAATGTTTGCGACTGATGATGACGTTATTGTCAATGAAATCGCCCCACGACCACATAA
CTCTGGGCACTATTCTATTGAAGCCTGTGATTTCTCTCAGTTTGACACCCATATTCTCGGTGTTCTCGGAGCACCATTAC
CAGTCATCAAACTCCATGCGCCAGCCGTTATGCTTAATGTCCTCGGTCAGCATGTCGAGGCTGCTGAGCAATATGTGACA
GAAAATTCAAGCGCCCACCTCCACATGTATGGTAAAATAGAAGCGAAGCACAACCGCAAGATGGGACATGTGACCTTGTT
TAGTGATGTACCGGATAGTGTGGATGAGCTTGGGGAAGGGATTGATTTTTAG

Upstream 100 bases:

>100_bases
CTCTTTGCCCTCCGTCTCCTCTCTGTAGAAGATAAGTCCATTGCGGATGCACTTGCCAACTTTGCTGAAGAACAAGGAAA
AATCGCAGAGGAGTCGTCAA

Downstream 100 bases:

>100_bases
GACAAGTCTATGATACAAATTATCGTTAATACATTTATTGAAAAGGATAAGACTGGAGCAGTTGTTGAAGTGTTGTATGC
CAGTGCTGACCAAGATAAGG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVAEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGL
DAVIKDGQLPQGTDLLRISQNRIFEKDFLSNKSQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPARISESLVDKAKAMAVRIAEQLN
LSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVT
ENSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVDELGEGIDF

Sequences:

>Translated_363_residues
MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVAEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGL
DAVIKDGQLPQGTDLLRISQNRIFEKDFLSNKSQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPARISESLVDKAKAMAVRIAEQLN
LSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVT
ENSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVDELGEGIDF
>Mature_362_residues
SSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVAEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLD
AVIKDGQLPQGTDLLRISQNRIFEKDFLSNKSQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEADL
EAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPARISESLVDKAKAMAVRIAEQLNL
SGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTE
NSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVDELGEGIDF

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=346, Percent_Identity=27.1676300578035, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788155, Length=359, Percent_Identity=25.6267409470752, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=33.0601092896175, Blast_Score=179, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006140
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C; PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39357; Mature: 39226

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVAEIIVAPYNDVDALRQ
CCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHEEEEEECCCCHHHHHH
LADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNRIFEKDFLSNKSQVTVAPY
HHHHCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEEEHH
KVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEADLEAAYALADSADCVLEEFVN
HHHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHC
FDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPARISESLVDKAKAMAVRIAEQLN
CCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
LSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPVIKLHA
CCCEEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECC
PAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVDELGEG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEECCCCHHHHHHCCC
IDF
CCC
>Mature Secondary Structure 
SSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVAEIIVAPYNDVDALRQ
CCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHEEEEEECCCCHHHHHH
LADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNRIFEKDFLSNKSQVTVAPY
HHHHCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEEEHH
KVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEADLEAAYALADSADCVLEEFVN
HHHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHC
FDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPARISESLVDKAKAMAVRIAEQLN
CCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
LSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPVIKLHA
CCCEEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECC
PAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVDELGEG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEECCCCHHHHHHCCC
IDF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA