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The map label for this gene is rsuA-2 [H]

Identifier: 116516226

GI number: 116516226

Start: 616398

End: 617123

Strand: Reverse

Name: rsuA-2 [H]

Synonym: SPD_0592

Alternate gene names: 116516226

Gene position: 617123-616398 (Counterclockwise)

Preceding gene: 116515352

Following gene: 116517096

Centisome position: 30.16

GC content: 43.39

Gene sequence:

>726_bases
ATGCGTTTAGATAATTTATTAGCCCAAGAAAAAATCAGCCGAAAGGCCATGAAGCAAGCACTCCTCAGAGGGAAAATTCT
AGTCGATGGTTGCCCAGCCCGCTCCCTAGCTCAAAATATCGATACAGGACTACAAGAACTCCTTTTTCAGGATCGAATCA
TTCAAGGCTATGAACACACCTATCTTATGCTTCATAAACCTGCTGGTGTCGTTACAGCCAACAAAGACAAGGAACTTCCG
ACCGTCATGGACCTGCTTCCATCTGACATCCAGTCTGACAAGCTCTATGCCGTTGGCCGACTGGACCGAGATACAACGGG
ACTCCTCCTCTTGACCGATAACGGTCCCTTGGGCTTTCAGCTCCTCCATCCCCAATATCATGTCGATAAGACTTACCAAG
TTGAGGTTAATGGACTTCTAACACCTGACCATATCCAAGCCTTTCAAAAGGGAATTGTCTTTTTAGATGGTACTGTCTGT
AAACCCGCAAAACTAGAGATTCTATCTGCAAGTCCCTCCATCAGTCAAGCCTCTATCACCATTTCAGAAGGAAAATTTCA
TCAAATCAAGAAAATGTTCCTCTCGGTTGGTGTTAAGGTGACTAGCCTCAAAAGAATCCAATTTGGGGACTTCACATTGA
ACCCAGATTTAGCAGAAAGTAACTACCGCCCTTTGAACCAAAAAGAGTTACAAATCATTAAAAACTATTTAGAGATGAGT
CGATAA

Upstream 100 bases:

>100_bases
TAAATAACATAACTCAAGCAAGTGTAAATGTTTTCACTCCGCTTTTCTTTTCACGTCAAGCCTTTTCAAAGCAAGCGACT
TATGATAAGATAGGCACAGT

Downstream 100 bases:

>100_bases
AACAAAAAAAGCTTTAAAACTAAAGCTTTTTTCTTTTATTTACCGAAAAATTAAGGCGATTGCTACAATCCAGTTAACTA
CAGAAATCACAATTCCTAAG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGLQELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELP
TVMDLLPSDIQSDKLYAVGRLDRDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAESNYRPLNQKELQIIKNYLEMS
R

Sequences:

>Translated_241_residues
MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGLQELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELP
TVMDLLPSDIQSDKLYAVGRLDRDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAESNYRPLNQKELQIIKNYLEMS
R
>Mature_241_residues
MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGLQELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELP
TVMDLLPSDIQSDKLYAVGRLDRDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAESNYRPLNQKELQIIKNYLEMS
R

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=36.6379310344828, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1787524, Length=182, Percent_Identity=31.3186813186813, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081838, Length=180, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=24.2553191489362, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27044; Mature: 27044

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGLQELLFQDRIIQGYEHT
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLDRDTTGLLLLTDNGPLGFQ
EEEEECCCCEEECCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEE
LLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVCKPAKLEILSASPSISQASIT
EECCEECCCCEEEEEECCCCCHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCEEEEE
ISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAESNYRPLNQKELQIIKNYLEMS
ECCCHHHHHHHHHHHHCCEEEEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
R
C
>Mature Secondary Structure
MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGLQELLFQDRIIQGYEHT
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLDRDTTGLLLLTDNGPLGFQ
EEEEECCCCEEECCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEE
LLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVCKPAKLEILSASPSISQASIT
EECCEECCCCEEEEEECCCCCHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCEEEEE
ISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAESNYRPLNQKELQIIKNYLEMS
ECCCHHHHHHHHHHHHCCEEEEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]