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The map label for this gene is gyrB

Identifier: 116516156

GI number: 116516156

Start: 723349

End: 725295

Strand: Direct

Name: gyrB

Synonym: SPD_0709

Alternate gene names: 116516156

Gene position: 723349-725295 (Clockwise)

Preceding gene: 116515873

Following gene: 116516893

Centisome position: 35.35

GC content: 42.78

Gene sequence:

>1947_bases
ATGACAGAAGAAATCAAAAATCTGCAGGCACAGGATTATGATGCCAGTCAAATTCAAGTTTTAGAGGGCTTAGAGGCTGT
TCGTATGCGTCCAGGGATGTACATTGGATCAACCTCAAAAGAAGGTCTTCACCATCTAGTCTGGGAAATTGTTGATAACT
CAATTGACGAGGCCTTGGCAGGATTTGCCAGCCATATTCAAGTTTTTATTGAGCCAGATGATTCGATTACTGTTGTGGAT
GATGGGCGTGGTATCCCAGTCGATATTCAGGAAAAAACAGGTCGTCCTGCTGTTGAGACCGTCTTTACAGTCCTTCACGC
TGGAGGAAAGTTCGGCGGTGGTGGATACAAGGTTTCAGGTGGTCTTCACGGGGTGGGGTCGTCAGTTGTTAATGCCCTTT
CCACTCAATTAGACGTTCATGTCCATAAAAACGGTAAGATTCATTACCAAGAATACCGTCGTGGTCATGTTGTCGCAGAT
CTTGAAATAGTTGGAGATACGGATAAAACAGGAACAACTGTTCACTTCACACCGGACCCAAAAATCTTCACTGAAACAAC
AATCTTTGATTTTGATAAATTAAATAAACGGATTCAAGAGTTGGCCTTTCTAAATCGCGGTCTTCAAATTTCTATCACTG
ATAAGCGCCAAGGTTTGGAACAAACCAAGCATTATCATTATGAAGGTGGGATTGCTAGTTACGTTGAATATATCAACGAG
AACAAGGATGTAATCTTTGATACACCAATCTATACAGACGGTGAGATGGATGATATCACAGTTGAGGTAGCCATGCAGTA
CACAACGGGTTACCATGAAAATGTCATGAGTTTCGCCAATAATATTCATACACATGAAGGTGGAACGCATGAACAAGGTT
TCCGTACAGCCTTGACACGTGTTATCAACGATTATGCTCGTAAGAATAAGTTACTGAAAGACAATGAAGACAATCTAACA
GGGGAAGATGTTCGCGAAGGCTTAACTGCAGTTATCTCAGTTAAACACCCAAATCCACAGTTTGAAGGACAAACGAAGAC
CAAATTGGGAAATAGCGAAGTGGTCAAGATTACCAATCGCCTCTTCAGTGAAGCCTTCTCCGATTTCCTCATGGAAAATC
CACAGATTGCCAAACGTATCGTAGAAAAAGGAATTTTGGCTGCCAAGGCTCGTGTGGCTGCCAAGCGTGCGCGTGAAGTC
ACACGTAAAAAATCTGGTTTGGAAATTTCCAACCTTCCAGGGAAACTAGCAGACTGTTCTTCTAATAACCCTGCTGAAAC
AGAACTCTTCATCGTCGAAGGAGACTCAGCTGGTGGATCAGCCAAATCTGGTCGTAACCGTGAGTTTCAGGCTATCCTTC
CAATTCGCGGTAAGATTTTGAACGTTGAAAAAGCAAGTATGGATAAGATTCTAGCTAACGAAGAAATTCGTAGTCTTTTC
ACAGCCATGGGAACAGGATTTGGCGCAGAATTTGATGTTTCGAAAGCCCGTTACCAAAAACTCGTTTTGATGACCGATGC
CGATGTCGATGGAGCCCACATTCGTACCCTTCTTTTAACCTTGATTTATCGTTATATGAAACCAATCCTAGAAGCTGGCT
ATGTTTATATTGCCCAACCACCAATCTATGGTGTCAAGGTTGGAAGCGAGATTAAAGAATATATCCAGCCGGGTGCAGAT
CAAGAAATCAAACTCCAAGAAGCTTTAGCCCGTTATAGTGAAGGTCGTACCAAACCGACTATTCAGCGTTATAAGGGGCT
AGGTGAAATGGACGATCATCAGCTGTGGGAAACAACCATGGATCCCGAACATCGCTTGATGGCTAGAGTTTCTGTAGATG
ATGCTGCAGAAGCAGATAAAATCTTTGATATGTTGATGGGGGATCGAGTAGAGCCTCGTCGTGAGTTTATCGAAGAAAAT
GCTGTCTATAGTACACTTGATGTCTAA

Upstream 100 bases:

>100_bases
TTGATATCGAAGCAGGTCAAGCTGCAGGACTTGATACCCACTTGTTTACCAGTATCGTGAATTTAAGACAAGTATTAGAC
ATATAAGAAAAAGGAATAAG

Downstream 100 bases:

>100_bases
AAAATAGGGAGAAGTAGTTCCCTTGGTCTAAAAAATATGATATAATCGTTACGATTGTTTCAAGTAAAAAAGGAGTTTGA
TATGTCTAATGGACAACTAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVD
DGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLT
GEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREV
TRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGAD
QEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEEN
AVYSTLDV

Sequences:

>Translated_648_residues
MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVD
DGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLT
GEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREV
TRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGAD
QEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEEN
AVYSTLDV
>Mature_647_residues
TEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDD
GRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVADL
EIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINEN
KDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTG
EDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREVT
RKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLFT
AMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQ
EIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEENA
VYSTLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI19913408, Length=678, Percent_Identity=25.6637168141593, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19913406, Length=665, Percent_Identity=25.4135338345865, Blast_Score=123, Evalue=7e-28,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=53.4513274336283, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1789408, Length=646, Percent_Identity=39.1640866873065, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=23.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17535065, Length=669, Percent_Identity=24.813153961136, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=235, Percent_Identity=25.1063829787234, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=595, Percent_Identity=25.2100840336134, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136538, Length=693, Percent_Identity=24.8196248196248, Blast_Score=136, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_STRPN (P0A4L9)

Other databases:

- EMBL:   X83917
- EMBL:   AE005672
- PIR:   F95093
- RefSeq:   NP_345303.1
- ProteinModelPortal:   P0A4L9
- EnsemblBacteria:   EBSTRT00000027034
- GeneID:   930756
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0806
- TIGR:   SP_0806
- GeneTree:   EBGT00050000028252
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK05644
- BioCyc:   SPNE170187-1:SP_0806-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 72238; Mature: 72107

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALA
CCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
GFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSG
HHHHCEEEEECCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVADLEIVGDTDKTGTTVHFTPDP
CCCHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCEEEEEEEECCCCCCCCEEEECCCC
KIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
CEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCEECCCCHHHHHHHHCC
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTR
CCCEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VINDYARKNKLLKDNEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNR
HHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHH
LFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREVTRKKSGLEISNLPGKLADCS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCC
SNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQP
HHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PIYGVKVGSEIKEYIQPGADQEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTM
CCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHCCC
DPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEENAVYSTLDV
CHHHHHEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALA
CCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
GFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSG
HHHHCEEEEECCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVADLEIVGDTDKTGTTVHFTPDP
CCCHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCEEEEEEEECCCCCCCCEEEECCCC
KIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
CEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCEECCCCHHHHHHHHCC
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTR
CCCEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VINDYARKNKLLKDNEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNR
HHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHH
LFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREVTRKKSGLEISNLPGKLADCS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCC
SNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQP
HHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PIYGVKVGSEIKEYIQPGADQEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTM
CCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHCCC
DPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEENAVYSTLDV
CHHHHHEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7608096; 11463916