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The map label for this gene is aspS

Identifier: 116516137

GI number: 116516137

Start: 1914397

End: 1916160

Strand: Reverse

Name: aspS

Synonym: SPD_1941

Alternate gene names: 116516137

Gene position: 1916160-1914397 (Counterclockwise)

Preceding gene: 116516424

Following gene: 116517145

Centisome position: 93.65

GC content: 45.63

Gene sequence:

>1764_bases
ATGAAACGTAGTATGTATGCTGGTCGTGTTCGTGAGGAACACATCGGACAAGAAATAACCTTGAAAGGATGGGTTGGCCG
TCGTCGTGACCTTGGTGGTTTGATCTTTATCGATCTTCGTGACCGTGAAGGAATCATGCAGTTGGTTATCAACCCTGAAA
AAGTATCTGCAGAGGTTATGGCAACAGCTGAAAGCCTTCGTAGCGAATTTGTTATTGAGGTGACTGGTCAGGTCGCTGCG
CGTGAGCAAGCCAATGATAAGTTGCCAACTGGTGCGGTTGAGTTAAACGTGACAGCTCTTATTGTGCTTAACACAGCTAA
GACAACACCATTTGAGATTAAGGATGGCATTGAGGCAAATGACGATACACGTTTGCGTTACCGTTACCTTGACCTTCGTC
GTCCAGAAATGTTGGAAAATCTTAAACTTCGTGCCAAGGTGACCCACTCTATCCGCAACTACTTGGATGAGTTGGAGTTT
ATCGACGTGGAGACACCATTCCTTTCTAAGTCAACGCCTGAAGGGGCGCGTGATTATTTAGTGCCGTCTCGTGTTAATAA
GGGGCATTTTTACGCTCTTCCTCAAAGTCCACAAATCACGAAACAGCTCTTGATGAATGCTGGTTTTGACCGTTACTACC
AAATCGTTAAATGTTTCCGTGACGAGGACTTGCGTGGAGACCGCCAGCCTGAATTTACTCAGGTCGACTTGGAAACGTCC
TTCCTTACTGAGCAAGAAATCCAAGATATTACAGAAGGCTTGATCGCGCGCGTGATGAAGGAAACAAAAGACATCGAAGT
AACGCTACCGTTCCCTCGTATGAAATACGATGATGCTATGGCTCTTTACGGTTCTGACAAGCCAGATACTCGTTTTGACA
TGTTGCTTCAGGACTTGACAGAAGTGGTCAAAGGTGTAGACTTTAAAGTCTTTTCAGAAGCACTTGCTGTAAAAGCGATT
GTGGTCAAAGGAGCTGCGGACAACTATTCACGTAAAGACATCGACAAGATGACGGAAGTAGCCAAACAGTATGGTGCCAA
AGGTCTTGCTTGGGTCAAGGTGGTTGATGGAGAATTAAACGGACCAGTTGCCAAGTTCTTGACTGGTATCCAAGAAGAAT
TGACAACAGCGCTTGCTCTTGAAGATAAGGACTTGGTTCTCTTTGTGGCGGATACGCTTGAAGTGGCTAATGCAACACTG
GGTGCCCTTCGTGGACGTATTGCTAAAGAGCTTGGCTTGATTGATAATGATAAGTTCAACTTCCTTTGGGTGGTTGACTG
GCCGATGTTTGAATGGTCTGAAGAAGAAGGCCGCTACATGAGCGCCCACCATCCATTCACACTTCCACAGGAAGAGACTG
CTCACGAATTAGAAGGTGATTTGGCTAAGGTTCGTGCCATTGCTTACGATATCGTCTTGAACGGTTATGAGCTTGGTGGT
GGTAGCCTTCGTATCAACCAAAAAGACCTTCAAGAACGCATGTTCAAGGCTCTTGGTTTCTCAGCTGAAGAAGCCAATGA
CCAGTTTGGTTTCCTTCTTGAAGCCATGGACTATGGTTTCCCACCACACGGTGGTTTGGCTATCGGGCTTGACCGTTTTG
TCATGTTGCTTGCTGGAGAAGAAAATATCCGTGAAGTCATTGCCTTTCCTAAGAACAACAAGGCAACTGACCCAATGACA
CAAGCTCCATCAACAGTCGCTCTCAAACAACTAGAGGAACTCAGCTTACAAGTAGAAGAAGATGAAACAAGCAAAACGAA
TTAA

Upstream 100 bases:

>100_bases
TCACCTGACTTTTAAAGACTGGCAAACTTTTCTGATGATTTTCATGAAGAGCCTGCGCTTTTATGGTAAAATAGTAACAG
AATAAAAGAGGAGAGAAATA

Downstream 100 bases:

>100_bases
GCGGTGGCGCTATTATCTGCGCCGCTTTGCTTATCAGATAAAAATTTTACGTGTCTTACAAAGTATCTCTCGAGAAAAGT
ATGATGAGAAGATTTCGGCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAA
REQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLTEQEIQDITEGLIARVMKETKDIEVTLPFPRMKYDDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAI
VVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATL
GALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMT
QAPSTVALKQLEELSLQVEEDETSKTN

Sequences:

>Translated_587_residues
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAA
REQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLTEQEIQDITEGLIARVMKETKDIEVTLPFPRMKYDDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAI
VVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATL
GALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMT
QAPSTVALKQLEELSLQVEEDETSKTN
>Mature_587_residues
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAA
REQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLTEQEIQDITEGLIARVMKETKDIEVTLPFPRMKYDDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAI
VVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATL
GALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMT
QAPSTVALKQLEELSLQVEEDETSKTN

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=41.4023372287145, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI45439306, Length=296, Percent_Identity=26.0135135135135, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI5031815, Length=282, Percent_Identity=26.9503546099291, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI194272210, Length=282, Percent_Identity=26.9503546099291, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=50.4230118443316, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1789256, Length=295, Percent_Identity=26.1016949152542, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790571, Length=252, Percent_Identity=25.3968253968254, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=35.5, Blast_Score=334, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=309, Percent_Identity=22.6537216828479, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=309, Percent_Identity=22.6537216828479, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=311, Percent_Identity=22.508038585209, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=301, Percent_Identity=24.5847176079734, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=302, Percent_Identity=26.158940397351, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=301, Percent_Identity=37.2093023255814, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=243, Percent_Identity=29.2181069958848, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=288, Percent_Identity=25.6944444444444, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24584738, Length=605, Percent_Identity=37.6859504132231, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=28.4518828451883, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=26.6932270916335, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=26.6932270916335, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_STRP2 (Q04I59)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817329.1
- ProteinModelPortal:   Q04I59
- SMR:   Q04I59
- STRING:   Q04I59
- EnsemblBacteria:   EBSTRT00000020541
- GeneID:   4442499
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1941
- eggNOG:   COG0173
- GeneTree:   EBGT00050000027607
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66256; Mature: 66256

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVM
CCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEAN
HHHHHHHCCEEEEECCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEFIDVETPFLSKSTPEGARDYL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHHCC
VPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLTEQEIQDITEGLIARVMKETKDIEVTLPFPRMKYDDAMALYGSDKPDTRFDMLLQDLT
HCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
EVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELN
HHHHCCCHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATLGALRGRIAKELGLIDNDKFN
CCHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGE
CEEEECHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
ENIREVIAFPKNNKATDPMTQAPSTVALKQLEELSLQVEEDETSKTN
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCC
>Mature Secondary Structure
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVM
CCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEAN
HHHHHHHCCEEEEECCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEFIDVETPFLSKSTPEGARDYL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHHCC
VPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLTEQEIQDITEGLIARVMKETKDIEVTLPFPRMKYDDAMALYGSDKPDTRFDMLLQDLT
HCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
EVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELN
HHHHCCCHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATLGALRGRIAKELGLIDNDKFN
CCHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGE
CEEEECHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
ENIREVIAFPKNNKATDPMTQAPSTVALKQLEELSLQVEEDETSKTN
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA