Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 116516062

GI number: 116516062

Start: 53975

End: 55522

Strand: Direct

Name: purH [H]

Synonym: SPD_0057

Alternate gene names: 116516062

Gene position: 53975-55522 (Clockwise)

Preceding gene: 116516890

Following gene: 116517132

Centisome position: 2.64

GC content: 48.0

Gene sequence:

>1548_bases
ATGACTAAACGCGTCTTAATCAGCGTCTCAGACAAAGCGGGCATTGTTGAATTTGCCCAAGAACTAAAAAAACTTGGTTG
GGAGATTATCTCGACAGGTGGAACTAAGGTTGCCCTTGATAATGCTGGGGTGGAGACCATTGCGATCGATGATGTGACGG
GCTTCCCAGAGATGATGGATGGCCGTGTTAAGACTCTTCATCCAAATATCCACGGGGGTCTTCTCGCTCGTCGTGATTTG
GATAGCCACTTGGAAGCAGCCAATGAGAACCAAATTGAGCTTATTGACCTTGTAGTGGTCAACCTTTATCCTTTCAAGGA
AACGATTCTCAAACCAGACGTGACGTACGCTGATGCTGTGGAGAATATTGATATTGGTGGGCCATCTATGCTTCGTTCAG
CAGCGAAGAATCATGCCAGCGTGACGGTTGTGGTAGACCCTGCTGACTATACAGTGGTTTTGGACGAGTTGTCAGCCAAT
GGTGAAACGACTTACGAAACGCGTCAACGTTTGGCAGCCAAGGTTTTCCGTCACACAGCGGCTTATGACGCTTTGATTGC
AGAATACTTCACAGCTCAAGTGGGCGAAAGTAAACCTGAAAAGTTGACCTTGACCTATGACCTAAAACAAGCTATGCGTT
ATGGGGAAAATCCCCAGCAGGATGCGGACTTCTACCAGAAAGCTTTGCCGACTGATTACTCCATTGCATCAGCCAAACAG
CTTAACGGTAAGGAATTGTCATTCAACAATATCCGCGATGCAGATGCTGCCATTCGTATTATCCGTGACTTCAAAGACCG
TCCAACCGTTGTGGCTCTCAAGCATATGAATCCATGTGGTATCGGCCAGGCGGATGACATCGAGACGGCTTGGGATTATG
CTTATGAGTCTGACCCAGTGTCTATCTTTGGTGGTATCGCTGTTCTTAACCGTGAGGTGGATGCTGCGACAGCTGAGAAG
ATGCACGGCGTTTTCCTCGAAATCATCATTGCACCAAGCTATACGGATGAAGCGCTAGCCATTTTGATCAATAAAAAGAA
AAACTTGCGTATCCTTGCCTTGCCATTTAATGCTCAAGAGGCTAGCGAAGTGGAAGCAGAATACACAGGTGTAGTCGGTG
GACTTCTCGTGCAAAATCAAGACGTGGTCAAGGAAAGCCCAGCTGACTGGCAAGTGGTGACCAAACGTCAGCCAACCGAG
ACAGAAGCGACTGCTCTTGAGTTCGCTTGGAAGGCTATCAAGTACGTCAAATCAAATGGTATTATCGTGACCAACGACCA
CATGACACTTGGTGTTGGTCCAGGTCAAACCAACCGTGTGGCTTCTGTTCGCCTTGCCATTGACCAAGCCAAAGATCGTC
TGGACGGGGCGGTCCTTGCTTCAGATGCCTTCTTCCCATTTGCGGATAACGTGGAAGAAATCGCCAAAGCAGGAATTAAG
GCCATCATCCAGCCCGGTGGCTCTGTCCGTGACCAAGAATCCATCGAAGCAGCGGATAAATACGGCTTGACTATGGTCTT
TACAGGTGTGAGACATTTTAGACATTAA

Upstream 100 bases:

>100_bases
GATCAATATCTTAGGTATGCTTAGCCTTGGTTTTGCTTATCTTGTTTTACTGTTACTGCATTTAATTGGTGTTTAACTAA
TGATTAAAAAGGAGAATATA

Downstream 100 bases:

>100_bases
GAAGATAAAAGGGAAGAAAACAGTTTCTTTCCTTTTTTGGCTTAAAATGCTAACTGAAACAAGATTAAAACGAACTTTTT
TGATATAATGTTGGTAAATA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGVETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDL
DSHLEAANENQIELIDLVVVNLYPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEK
MHGVFLEIIIAPSYTDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQVVTKRQPTE
TEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQAKDRLDGAVLASDAFFPFADNVEEIAKAGIK
AIIQPGGSVRDQESIEAADKYGLTMVFTGVRHFRH

Sequences:

>Translated_515_residues
MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGVETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDL
DSHLEAANENQIELIDLVVVNLYPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEK
MHGVFLEIIIAPSYTDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQVVTKRQPTE
TEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQAKDRLDGAVLASDAFFPFADNVEEIAKAGIK
AIIQPGGSVRDQESIEAADKYGLTMVFTGVRHFRH
>Mature_514_residues
TKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGVETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLD
SHLEAANENQIELIDLVVVNLYPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSANG
ETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQL
NGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKM
HGVFLEIIIAPSYTDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQVVTKRQPTET
EATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQAKDRLDGAVLASDAFFPFADNVEEIAKAGIKA
IIQPGGSVRDQESIEAADKYGLTMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=475, Percent_Identity=37.6842105263158, Blast_Score=255, Evalue=6e-68,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=48.9603024574669, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71985564, Length=476, Percent_Identity=34.453781512605, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI71985556, Length=74, Percent_Identity=45.945945945946, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71985574, Length=310, Percent_Identity=26.1290322580645, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323768, Length=477, Percent_Identity=38.1551362683438, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6323056, Length=476, Percent_Identity=34.453781512605, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24649832, Length=479, Percent_Identity=38.4133611691023, Blast_Score=272, Evalue=4e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56383; Mature: 56252

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGVETIAIDDVTGFPEMMD
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEECCCCCCHHHHC
GRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNLYPFKETILKPDVTYADAV
CCEEEECCCCCCCEEECCCHHHHHHHCCCCCEEEEEEEEEEEECCHHHHCCCCCCHHHHH
ENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSANGETTYETRQRLAAKVFRHTA
CCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
HHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYAYESDPV
CCCCEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCH
SIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSYTDEALAILINKKKNLRILALPFNAQE
HHHCCCHHEECCCCHHHHHHHCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCC
ASEVEAEYTGVVGGLLVQNQDVVKESPADWQVVTKRQPTETEATALEFAWKAIKYVKSNG
HHHHHHHHHHHHHHEEECCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
IIVTNDHMTLGVGPGQTNRVASVRLAIDQAKDRLDGAVLASDAFFPFADNVEEIAKAGIK
EEEECCCEEEEECCCCCCCEEEEEEEHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHCHH
AIIQPGGSVRDQESIEAADKYGLTMVFTGVRHFRH
EEECCCCCCCCHHHHHHHHHCCEEHEEHHHHHHCC
>Mature Secondary Structure 
TKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGVETIAIDDVTGFPEMMD
CCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEECCCCCCHHHHC
GRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNLYPFKETILKPDVTYADAV
CCEEEECCCCCCCEEECCCHHHHHHHCCCCCEEEEEEEEEEEECCHHHHCCCCCCHHHHH
ENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSANGETTYETRQRLAAKVFRHTA
CCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
HHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYAYESDPV
CCCCEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCH
SIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSYTDEALAILINKKKNLRILALPFNAQE
HHHCCCHHEECCCCHHHHHHHCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCC
ASEVEAEYTGVVGGLLVQNQDVVKESPADWQVVTKRQPTETEATALEFAWKAIKYVKSNG
HHHHHHHHHHHHHHEEECCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
IIVTNDHMTLGVGPGQTNRVASVRLAIDQAKDRLDGAVLASDAFFPFADNVEEIAKAGIK
EEEECCCEEEEECCCCCCCEEEEEEEHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHCHH
AIIQPGGSVRDQESIEAADKYGLTMVFTGVRHFRH
EEECCCCCCCCHHHHHHHHHCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA