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The map label for this gene is ilvE [H]

Identifier: 116516002

GI number: 116516002

Start: 762382

End: 763404

Strand: Direct

Name: ilvE [H]

Synonym: SPD_0749

Alternate gene names: 116516002

Gene position: 762382-763404 (Clockwise)

Preceding gene: 116515631

Following gene: 116515710

Centisome position: 37.26

GC content: 41.35

Gene sequence:

>1023_bases
ATGACAGTAACGATTGATTGGGAAAACCTCGGTTTTTCCTATATGAAATTACCTTATCGCTATCTTGCTCATTTCAAAAA
TGGACAATGGGATCAAGGAGAGCTTACAGAGGATGCAACTTTGCATATTTCAGAGTCTTCTCCAAGTCTTCACTATGGAC
AACAAGCATTTGAAGGTTTGAAAGCTTATCGTACTAAGGATGGCAGTGTTCAACTGTTCCGTCCTGATGAAAATGCTAAA
CGCCTGCAACGTACATGTGACCGTCTCTTGATGCCACAAGTTCCGACAGACATGTTTGTAGAAGCTTGTAAAGCAGTTGT
CCGTGCGAATGAAGAATACGTACCACTATACGGAATAGGTGGAACCTTATATCTTCGCCCTCTTTTGATTGGTGTCGGAG
ATATTATCGGGGTAAAACCGGCAGAAGAGTACATTTTCACCATCTTTGCTATGCCAGTTGGAAATTACTTTAAAGGTGGT
TTGGTCCCAACCAACTTCTTGATTCAGGATGAGTACGACCGTGCAGCACCAAATGGTACAGGTGCGGCTAAGGTTGGTGG
AAACTATGCTGCAAGTCTCTTACCAGGAAAAATGGCCAAGTCACGCCATTTCTCAGATGTTATCTATCTGGACCCATCAA
CTCATACAAAGATTGAAGAAGTCGGATCAGCTAATTTCTTTGGAATTACAGCTGATAATGAATTTGTAACACCATTGAGT
CCATCTATCTTGCCATCTATTACCAAGTATTCCTTGCTTTATTTGGCAGAACATCGCTTGGGATTAACTCCTATTGAGGG
TGATGTTCCAATTGATAATCTTGACCGTTTTGTAGAGGCAGGTGCCTGTGGTACAGCAGCGGTTATTTCTCCAATTGGAG
GTATTCAACATGGTGATGATTTCCATGTATTCTATAGTGAAACAGAAGTAGGTCCTGTGACGCGTAAATTATATAATGAA
TTGACGGGTATTCAGTTTGGCGATATTGAAGCGCCAGAAGGTTGGATTGTAAAAGTAGATTAA

Upstream 100 bases:

>100_bases
TTTTATTGGAGGAATTTTCTGAAAATTACAAAATATACTTGCTATTTTAGAAAAATAGTGTAGAATATAAGAAATAGGTT
TTTAGAATAAGGAGTGGAAT

Downstream 100 bases:

>100_bases
AATAAACCAAAGGAGATTTTTTATGAAATAGAAAAAGTGGCTCTTAACAGCAGGAGTGGTCCTGAGCACGTTAGCTATTT
TAGTGGCTTGTGGAAAAACT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAK
RLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFFGITADNEFVTPLS
PSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNE
LTGIQFGDIEAPEGWIVKVD

Sequences:

>Translated_340_residues
MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAK
RLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFFGITADNEFVTPLS
PSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNE
LTGIQFGDIEAPEGWIVKVD
>Mature_339_residues
TVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKR
LQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGL
VPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFFGITADNEFVTPLSP
SILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNEL
TGIQFGDIEAPEGWIVKVD

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=316, Percent_Identity=31.9620253164557, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI296010906, Length=316, Percent_Identity=31.9620253164557, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI296010904, Length=316, Percent_Identity=31.9620253164557, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI50658084, Length=333, Percent_Identity=33.033033033033, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI258614015, Length=300, Percent_Identity=34, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI296010902, Length=247, Percent_Identity=31.9838056680162, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI296010900, Length=247, Percent_Identity=31.9838056680162, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI48994963, Length=322, Percent_Identity=32.9192546583851, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=326, Percent_Identity=31.5950920245399, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17565728, Length=342, Percent_Identity=28.3625730994152, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322608, Length=320, Percent_Identity=32.1875, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6322002, Length=319, Percent_Identity=31.0344827586207, Blast_Score=149, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24641779, Length=348, Percent_Identity=32.4712643678161, Blast_Score=161, Evalue=7e-40,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37502; Mature: 37371

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGL
CEEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
KAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIG
HHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
GTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLVPTNFLIQDEYDRAAPNGT
HHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCHHCCCCCCCEEEEECCHHHCCCCCC
GAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFFGITADNEFVTPLS
CCEECCCCEEHHHCCCHHHHCCCCCEEEEECCCCCCHHHHCCCCCEEEEECCCCEECCCC
PSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAGACGTAAVISPIGGIQHGDD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
FHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEGWIVKVD
EEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCEEEEEC
>Mature Secondary Structure 
TVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGL
EEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
KAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIG
HHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
GTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLVPTNFLIQDEYDRAAPNGT
HHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCHHCCCCCCCEEEEECCHHHCCCCCC
GAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFFGITADNEFVTPLS
CCEECCCCEEHHHCCCHHHHCCCCCEEEEECCCCCCHHHHCCCCCEEEEECCCCEECCCC
PSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAGACGTAAVISPIGGIQHGDD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
FHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEGWIVKVD
EEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]