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The map label for this gene is asnA

Identifier: 116515784

GI number: 116515784

Start: 1766150

End: 1767142

Strand: Reverse

Name: asnA

Synonym: SPD_1768

Alternate gene names: 116515784

Gene position: 1767142-1766150 (Counterclockwise)

Preceding gene: 116516865

Following gene: 116516705

Centisome position: 86.37

GC content: 43.4

Gene sequence:

>993_bases
ATGAAGAAAAGTTTTATCCATCAACAAGAAGAAATTTCCTTTGTCAAAAACACTTTTACCCAGTATTTGAAAGATAAGCT
AGAAGTTGTCGAAGTTCAAGGTCCTATCTTGAGTAAGGTCGGTGACGGAATGCAGGACAACCTGTCTGGTGTGGAAAATC
CAGTATCGGTCAAGGTTCTCCAAATCCCTGATGCTACTTATGAAGTGGTGCACTCACTTGCTAAATGGAAACGCCACACC
TTGGCTCGTTTTGGCTTTGGTGAAGGAGAGGGTCTCTTTGTCCACATGAAAGCCCTTCGTCCAGATGAGGATTCCTTGGA
TGCAACCCACTCTGTTTATGTTGACCAGTGGGACTGGGAGAAGGTTATCCCAAATGGTAAGCGTAACATCGTTTATCTAA
AAGAAACAGTTGAGAAGATTTATAAGGCTATTCGCCTGACTGAGCTAGCTGTTGAAGCTCGCTATGACATCGAGTCTATC
TTGCCAAAACAAATCACCTTTATCCATACAGAAGAATTGGTAGAACGCTATCCAGACTTGACATCGAAAGAACGTGAAAA
TGCGATTTGTAAAGAATTTGGAGCCGTCTTTTTGATTGGTATCGGTGGCGAGTTGCCAGATGGTAAACCGCACGATGGAC
GTGCACCAGACTATGATGACTGGACAAGCGAGTCTGAGAATGGCTACAAGGGTCTAAATGGTGATATTCTTGTCTGGAAT
GAGTCTTTAGGTGGAGCCTTTGAGTTGTCTTCTATGGGAATTCGTGTAGATGAAGAAACTCTTAGACGTCAGGTGGAAAT
TACAGGTGATGAAGACCGCTTGGAATTGGAATGGCACAAGTCTTTGTTGAATGGCCTATTCCCATTGACAATCGGTGGAG
GAATTGGACAATCTCGTATGGCCATGTTCCTACTTCGCAAGAGACACATCGGAGAAGTGCAAACAAGTGTTTGGCCTCAA
GAAGTACGCGATACTTACGAAAATATTTTGTAG

Upstream 100 bases:

>100_bases
AATCAGTGTTTTGAGTGACACTGATTTTTTTTAAATTTTCTCTTTTCTTTTCTTGGAAATAGGTGTATAATACGTTTATT
AAATTTTTGAGGAGTTGTCT

Downstream 100 bases:

>100_bases
AGAATCGAACCGCAAGGTTCGGTTTTCTTTCTCTTTTTGTCTATAATTTGGTATAATAAACAGTATGAAAATCGTATCAG
GAATCTATGGGGGACGTCCC

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGMQDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHT
LARFGFGEGEGLFVHMKALRPDEDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDYDDWTSESENGYKGLNGDILVWN
ESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLELEWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQ
EVRDTYENIL

Sequences:

>Translated_330_residues
MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGMQDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHT
LARFGFGEGEGLFVHMKALRPDEDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDYDDWTSESENGYKGLNGDILVWN
ESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLELEWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQ
EVRDTYENIL
>Mature_330_residues
MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGMQDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHT
LARFGFGEGEGLFVHMKALRPDEDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDYDDWTSESENGYKGLNGDILVWN
ESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLELEWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQ
EVRDTYENIL

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily

Homologues:

Organism=Escherichia coli, GI1790183, Length=330, Percent_Identity=60.9090909090909, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNA_STRP2 (Q04IJ8)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817190.1
- ProteinModelPortal:   Q04IJ8
- SMR:   Q04IJ8
- STRING:   Q04IJ8
- EnsemblBacteria:   EBSTRT00000020409
- GeneID:   4441684
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1768
- eggNOG:   COG2502
- GeneTree:   EBGT00050000027962
- HOGENOM:   HBG288146
- OMA:   LNDNLNG
- ProtClustDB:   PRK05425
- GO:   GO:0005737
- HAMAP:   MF_00555
- InterPro:   IPR006195
- InterPro:   IPR004618
- PIRSF:   PIRSF001555
- TIGRFAMs:   TIGR00669

Pfam domain/function: PF03590 AsnA

EC number: =6.3.1.1

Molecular weight: Translated: 37608; Mature: 37608

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGMQDNLSGVENPVSVKVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCHHHHHHCCCCCCEEEEE
QIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPDEDSLDATHSVYVDQWDWE
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHH
KVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESILPKQITFIHTEELVERYPDL
HHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHEEEEHHHHHHHCCCC
TSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDYDDWTSESENGYKGLNGDILVWN
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLELEWHKSLLNGLFPLTIGGGIGQSRM
CCCCCEEECCCCCCEECHHHHHCEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHH
AMFLLRKRHIGEVQTSVWPQEVRDTYENIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGMQDNLSGVENPVSVKVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCHHHHHHCCCCCCEEEEE
QIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPDEDSLDATHSVYVDQWDWE
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHH
KVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESILPKQITFIHTEELVERYPDL
HHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHEEEEHHHHHHHCCCC
TSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDYDDWTSESENGYKGLNGDILVWN
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLELEWHKSLLNGLFPLTIGGGIGQSRM
CCCCCEEECCCCCCEECHHHHHCEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHH
AMFLLRKRHIGEVQTSVWPQEVRDTYENIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA