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The map label for this gene is purA [H]

Identifier: 116515772

GI number: 116515772

Start: 22635

End: 23921

Strand: Direct

Name: purA [H]

Synonym: SPD_0024

Alternate gene names: 116515772

Gene position: 22635-23921 (Clockwise)

Preceding gene: 116515956

Following gene: 116516620

Centisome position: 1.11

GC content: 40.71

Gene sequence:

>1287_bases
ATGACTTCAGTTGTTGTTGTAGGTACCCAATGGGGTGATGAAGGTAAAGGGAAGATTACAGACTTCCTTTCAGCGAATGC
AGAAGTGATTGCACGTTACCAAGGTGGTGATAATGCTGGTCACACGATTGTGATTGACGGTAAGAAATTTAAGTTGCACT
TGATTCCATCTGGGATTTTCTTCCCTGAAAAAATATCTGTCATTGGGAATGGTATGGTTGTAAATCCTAAATCTCTTGTA
AAAGAGTTGAGCTATCTTCATGAGGAAGGTGTAACAACTGATAACTTGCGTATTTCTGATCGTGCGCATGTTATTTTGCC
TTATCATATCGAGTTGGATCGCTTGCAAGAAGAAGCTAAGGGCGACAATAAGATTGGTACGACAATTAAGGGAATTGGTC
CAGCTTATATGGACAAGGCTGCTCGTGTTGGAATTCGTATTGCAGATCTTTTAGATAAAGATATTTTCCGTGAGCGTTTA
GAACGTAACCTTGCTGAAAAGAATCGTCTTTTTGAAAAATTGTATGACAGTAAAGCGATTGTTTTCGATGATATTTTTGA
AGAATATTACGAATATGGTCAACAAATCAAGAAATACGTGATAGATACATCTGTTATCTTGAATGATGCGCTTGATAATG
GCAAACGTGTGCTTTTTGAAGGTGCACAAGGTGTTATGCTAGATATCGACCAAGGTACTTATCCATTTGTTACGTCATCA
AACCCTGTAGCTGGTGGTGTGACAATTGGTTCTGGTGTCGGTCCAAGCAAGATTGACAAGGTTGTAGGTGTATGTAAAGC
TTATACGAGTCGTGTAGGAGATGGTCCTTTCCCAACTGAGTTGTTTGATGAAGTGGGAGAACGTATCCGTGAAGTGGGTC
ATGAGTATGGTACAACAACTGGTCGTCCACGTCGTGTAGGTTGGTTTGACTCAGTTGTGATGCGTCATAGCCGTCGTGTT
TCTGGTATTACTAACCTTTCTTTGAACTCTATTGATGTTTTGAGCGGTTTGGATACTGTGAAAATCTGTGTGGCCTATGA
TCTTGACGGTCAACGTATTGACTACTATCCAGCTAGTCTTGAGCAATTGAAACGTTGCAAGCCTATCTATGAAGAGTTGC
CAGGTTGGTCAGAAGATATTACCGGAGTTCGCAATTTGGAAGATCTTCCTGAGAATGCGCGTAACTATGTTCGTCGTGTG
AGTGAATTGGTTGGCGTTCGTATTTCTACTTTCTCAGTAGGTCCTGGTCGTGAACAAACAAATATTTTAGAAAGTGTTTG
GTCCTAA

Upstream 100 bases:

>100_bases
AAAGGCGATTTTTTATTATAATAAATTGTAAGATATAATTGCAGGTGAGAGTCCTGCCATGTATGTGAGAAAGGAAGAGC
CTGAGGGCTCAGACAAGATT

Downstream 100 bases:

>100_bases
GAGATTTTTAAGATTTGTTTAAGATAGGTCGGGTATACTATAGACGGTTACAAGAAGACCTCCTAACTTGTTGTAACAAA
TATCCTAAACTTTTCTTTTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLV
KELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRV
SGITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPENARNYVRRV
SELVGVRISTFSVGPGREQTNILESVWS

Sequences:

>Translated_428_residues
MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLV
KELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRV
SGITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPENARNYVRRV
SELVGVRISTFSVGPGREQTNILESVWS
>Mature_427_residues
TSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVK
ELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERLE
RNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVMLDIDQGTYPFVTSSN
PVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVS
GITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPENARNYVRRVS
ELVGVRISTFSVGPGREQTNILESVWS

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=46.0807600950119, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI34577063, Length=423, Percent_Identity=42.5531914893617, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=44.8979591836735, Blast_Score=326, Evalue=3e-89,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=48.2269503546099, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146555, Length=425, Percent_Identity=43.5294117647059, Blast_Score=341, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI25146553, Length=425, Percent_Identity=43.5294117647059, Blast_Score=341, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=424, Percent_Identity=42.2169811320755, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI21356033, Length=418, Percent_Identity=43.7799043062201, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47571; Mature: 47439

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIF
CCCEEEEEECCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
FPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAK
CCCHHHEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEECCHHHHHHHHC
GDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERLERNLAEKNRLFEKLYDSKAI
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVMLDIDQGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEEECCCCCEEECCCHHH
EQLKRCKPIYEELPGWSEDITGVRNLEDLPENARNYVRRVSELVGVRISTFSVGPGREQT
HHHHHCCHHHHHCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
NILESVWS
HHHHHHCC
>Mature Secondary Structure 
TSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIF
CCEEEEEECCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
FPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAK
CCCHHHEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEECCHHHHHHHHC
GDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERLERNLAEKNRLFEKLYDSKAI
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVMLDIDQGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEEECCCCCEEECCCHHH
EQLKRCKPIYEELPGWSEDITGVRNLEDLPENARNYVRRVSELVGVRISTFSVGPGREQT
HHHHHCCHHHHHCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
NILESVWS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA