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The map label for this gene is livJ [H]

Identifier: 116515756

GI number: 116515756

Start: 669986

End: 671146

Strand: Direct

Name: livJ [H]

Synonym: SPD_0652

Alternate gene names: 116515756

Gene position: 669986-671146 (Clockwise)

Preceding gene: 116516631

Following gene: 116515326

Centisome position: 32.74

GC content: 41.43

Gene sequence:

>1161_bases
ATGAAGAAAAAATTTGCCCTATCGTTTGTGGCGCTTGCAAGTGTAGCACTTCTTGCAGCCTGTGGAGAAGTGAAGTCTGG
AGCAGTCAACACTGCTGGTAACTCAGTAGAGGAAAAGACAATTAAAATCGGGTTTAACTTTGAAGAATCAGGTTCTTTAG
CTGCATACGGAACAGCTGAACAAAAAGGTGCCCAATTGGCTGTTGATGAAATCAATGCCGCAGGTGGTATCGATGGAAAA
CAAATCGAAGTAGTCGATAAAGATAATAAGTCTGAAACAGCTGAGGCTGCTTCAGTTACAACTAACCTTGTAACCCAATC
TAAAGTATCAGCAGTCGTAGGACCTGCGACATCTGGTGCGACTGCAGCTGCGGTAGCGAACGCTACAAAAGCAGGTGTTC
CATTGATCTCACCAAGTGCGACTCAAGATGGATTGACTAAAGGTCAAGATTACCTCTTTATTGGAACTTTCCAAGATAGC
TTCCAAGGAAAAATTATCTCAAACTATGTTTCTGAAAAATTAAATGCTAAGAAAGTTGTTCTTTACACTGACAATGCCAG
TGACTATGCTAAAGGGATTGCCAAATCTTTCCGCGAGTCATACAAGGGTGAAATCGTTGCAGATGAAACTTTCGTAGCAG
GTGACACAGACTTCCAAGCAGCCCTTACAAAAATGAAAGGGAAAGACTTTGATGCTATCGTTGTTCCTGGTTACTATAAT
GAGGCTGGTAAGATTGTAAACCAAGCGCGTGGTATGGGAATTGACAAACCAATCGTTGGTGGTGATGGATTCAACGGTGA
GGAGTTTGTACAACAAGCAACTGCTGAAAAAGCATCAAACATCTACTTTATCTCAGGCTTCTCAACTACTGTAGAAGTTT
CAGCTAAAGCTAAAGCCTTCCTTGACGCTTACCGTGCTAAGTACAATGAAGAGCCTTCAACATTTGCAGCCTTGGCTTAT
GATTCAGTTCACCTTGTAGCAAACGCAGCAAAAGGTGCTAAAAATTCAGGTGAAATCAAGAATAACCTTGCTAAAACAAA
AGATTTTGAAGGTGTAACTGGTCAAACAAGCTTCGATGCAGACCACAACACAGTCAAAACTGCTTACATGATGACCATGA
ACAATGGTAAAGTTGAAGCAGCAGAAGTTGTAAAACCATAA

Upstream 100 bases:

>100_bases
AAAACGCTATCATCGAAAAAGTTCAAGGAAAACATTGACAATTTTCTGATAATTCGGTATATTCTTAACAGACTATTTAA
GAAATAAGGAGACAAAAAAG

Downstream 100 bases:

>100_bases
TAGAAAAATGTTGAAATAGGGAATGAGCCTTTGACTCACTCCCTGTTTCGATATTTAATACTCTTCGAAAATCTCTTCAA
ACTGCGTCAACGTCGCCTTG

Product: branched-chain amino acid ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIGFNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGK
QIEVVDKDNKSETAEAASVTTNLVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDFQAALTKMKGKDFDAIVVPGYYN
EAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAEKASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAY
DSVHLVANAAKGAKNSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP

Sequences:

>Translated_386_residues
MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIGFNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGK
QIEVVDKDNKSETAEAASVTTNLVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDFQAALTKMKGKDFDAIVVPGYYN
EAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAEKASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAY
DSVHLVANAAKGAKNSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP
>Mature_386_residues
MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIGFNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGK
QIEVVDKDNKSETAEAASVTTNLVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDFQAALTKMKGKDFDAIVVPGYYN
EAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAEKASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAY
DSVHLVANAAKGAKNSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=348, Percent_Identity=27.0114942528736, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789867, Length=227, Percent_Identity=29.5154185022026, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40414; Mature: 40414

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIGFNFEESGSLAAYGTAE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCH
QKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTNLVTQSKVSAVVGPATSGA
HCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCC
TAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDSFQGKIISNYVSEKLNAKKVV
HHHHHHHHHHCCCCEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEE
LYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDFQAALTKMKGKDFDAIVVPGYYN
EEECCCHHHHHHHHHHHHHHHCCCEEECCEEEECCCHHHHHHHHHCCCCCCEEEECCCCC
EAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAEKASNIYFISGFSTTVEVSAKAKAF
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEECCCEEEEECHHHHHH
LDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAKNSGEIKNNLAKTKDFEGVTGQTSFDA
HHHHHHHCCCCCCCEEEEECCCCEEEEEHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
DHNTVKTAYMMTMNNGKVEAAEVVKP
CCCEEEEEEEEEECCCCEEHHHCCCC
>Mature Secondary Structure
MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIGFNFEESGSLAAYGTAE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCH
QKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTNLVTQSKVSAVVGPATSGA
HCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCC
TAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDSFQGKIISNYVSEKLNAKKVV
HHHHHHHHHHCCCCEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEE
LYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDFQAALTKMKGKDFDAIVVPGYYN
EEECCCHHHHHHHHHHHHHHHCCCEEECCEEEECCCHHHHHHHHHCCCCCCEEEECCCCC
EAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAEKASNIYFISGFSTTVEVSAKAKAF
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEECCCEEEEECHHHHHH
LDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAKNSGEIKNNLAKTKDFEGVTGQTSFDA
HHHHHHHCCCCCCCEEEEECCCCEEEEEHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
DHNTVKTAYMMTMNNGKVEAAEVVKP
CCCEEEEEEEEEECCCCEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]