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The map label for this gene is queA

Identifier: 116515685

GI number: 116515685

Start: 1274724

End: 1275752

Strand: Direct

Name: queA

Synonym: SPD_1247

Alternate gene names: 116515685

Gene position: 1274724-1275752 (Clockwise)

Preceding gene: 116515504

Following gene: 116516820

Centisome position: 62.3

GC content: 47.62

Gene sequence:

>1029_bases
ATGAATACAGCTGATTTTGATTTCCACTTGCCAGAGGAATTGATTGCCCAAACGCCCCTTGAAAAACGTGATGCCTCCAA
ACTCCTCATCGTCAACCGTGAGACAGGAGAAATGCAAGATAAACACTTCCACTCTATTATTGATATGCTGGAACCTGGTG
ATGCCCTTGTCATGAACGACACCCGAGTTCTCCCTGCCCGCCTCTATGGTCAAAAAGTGGAGACAGGAAGTCATGTGGAA
CTTCTCCTCCTTAAGAACACTAGTGGAGACGAGTGGGAAGTTCTGGCTAAACCTGCCAAACGCCTCAAGGTCGGTACTCG
TATCAGCTTTGGTGATGGCCGCCTCAGCGCTGTCGTTACAGAAGAATTGACCCACGGGGGACGCATTGTCCGCTTTGAAT
ACCAAAGAATTTTCCTAGAAGTCTTGGAAAGTCTGGGAGAAATGCCTCTGCCACCTTATATCCACGAAAAATTAGATGAC
CGTGAACGTTATCAAACCGTCTACGCCAAGGAAAGCGGCTCTGCTGCAGCACCGACTGCTGGTCTTCACTTCACCAAAGA
ACTGCTGGCAGAAATCCAAGCTAAGGGTGTCCATCTAGTCTATCTGACTCTCCATGTCGGACTCGGAACCTTTAGACCTG
TTTCTGTGGATAATCTGGACGAACACGAAATGCACTCAGAGTTCTATCAACTTTCTGAGGAAGCTGCGGCCACCCTTCGC
TCTGTCAAAGAAAATGGTGGTCGTGTCATCGCTGTCGGAACCACTTCTATCCGTACCTTGGAAACTATTGGTTCCAAGTT
TGATGGGCAAATCCAAGCAGATTCTGGTTGGACCAATATCTTTATCAAACCTGGGTATGAGTGGAAGGTCGTGGATGCCT
TCTCAACCAACTTCCACCTGCCAAAATCAACTCTGGTCATGTTGGTTTCTGCCTTTGCAGGCCGTGAATTAGTCTTAGAT
GCCTACCACCATTCCATCCAAGAACACTACCGCTTCTTCAGTTTTGGTGACGCCATGTTTATTTATTGA

Upstream 100 bases:

>100_bases
TTTGTCAAGTGAAAACGATTTAATTTCTAAAAAAAGAGCGTCCAAACTTGAAACGTGTTATAATGGATAGGATTAGAACT
TAGAAAGAATGAACAGATAT

Downstream 100 bases:

>100_bases
GAAAGAATTTCTCTAAATCTTCTAATACCAATAAATCGCTAAGATATTATTTCAAAGAACATCTACAATTGAAACTCTAG
CTAGCTGTAGAAGACGCCTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVE
LLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDD
RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
SVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLD
AYHHSIQEHYRFFSFGDAMFIY

Sequences:

>Translated_342_residues
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVE
LLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDD
RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
SVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLD
AYHHSIQEHYRFFSFGDAMFIY
>Mature_342_residues
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVE
LLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDD
RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
SVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLD
AYHHSIQEHYRFFSFGDAMFIY

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=46.3976945244957, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_STRP2 (Q04JT4)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816714.1
- ProteinModelPortal:   Q04JT4
- SMR:   Q04JT4
- STRING:   Q04JT4
- EnsemblBacteria:   EBSTRT00000019246
- GeneID:   4442551
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1247
- eggNOG:   COG0809
- GeneTree:   EBGT00050000028225
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38510; Mature: 38510

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVLPARLYGQKVETGSHVELLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVT
CEECHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHCEECCEEEECCCCEEEEEE
EELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDDRERYQTVYAKESGSAAAPTA
HHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHEEEEECCCCCCCCCC
GLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
CHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCC
PKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFSFGDAMFIY
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVLPARLYGQKVETGSHVELLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVT
CEECHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHCEECCEEEECCCCEEEEEE
EELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDDRERYQTVYAKESGSAAAPTA
HHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHEEEEECCCCCCCCCC
GLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
CHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCC
PKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFSFGDAMFIY
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA