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The map label for this gene is lacE-2 [H]

Identifier: 116515590

GI number: 116515590

Start: 1076183

End: 1077874

Strand: Reverse

Name: lacE-2 [H]

Synonym: SPD_1047

Alternate gene names: 116515590

Gene position: 1077874-1076183 (Counterclockwise)

Preceding gene: 116516599

Following gene: 116516678

Centisome position: 52.68

GC content: 40.54

Gene sequence:

>1692_bases
ATGAACAAACTAATTGCATTTATCGAGAAAGGAAAGCCTTTCTTTGATAAACTATCTCGTAATATCTATCTTCGTGCTAT
TCGTGATGGTTTCATTGCAGGTATGCCTGTTATTCTCTTCTCAAGTATCTTTATCTTGATTGCCTTTGTACCAAACTCAT
GGGGCTTTAAATGGTCTGATGAAGTTGTAGCCTTTCTGATGAAACCTTATAGCTATTCTATGGGTATTCTGGCTCTCTTG
GTAGCTGGTACAACAGCTAAGTCATTGACCGACTCAGTAAACCGTAGCATGGAGAAAACCAATCAAATCAACTATATGTC
AACGCTGTTAGCAGCGATTGTTGGTTTGTTGATGTTGGCAGCTGATCCTATCGAAAATGGTCTAGCTACTGGATTCTTGG
GGACAAAAGGTTTGCTTTCAGCCTTCCTTGCTGCCTTTGTTACTGTAGCCATCTATAAGGTCTGTGTTAAGAACAACGTC
ACTATTCGTATGCCTGACGAAGTTCCACCAAATATCTCACAAGTCTTTAAAGATGTGATTCCATTCACTCTATCAGTGGT
TTCTCTTTATGCTCTTGATTTACTAGCTCGTCATTTTGTTGGTTCTAGTGTGGCAGAATCAATCGGTAAATTCTTCGCAC
CACTCTTCTCAGCAGCAGACGGATACCTTGGTATTACCATTATCTTTGGTGCCTTTGCCTTCTTCTGGTTTGTGGGTATT
CATGGTCCATCTATTGTTGAACCTGCTATCGCAGCGATTACCTATGCAAATGCTGAAGTCAACTTGAACCTTCTCCAACA
AGGTATGCATGCTGATAAAATCCTTACTTCAGGAACTCAAATGTTTATCGTTACTATGGGAGGTACTGGTGCGACACTGG
TTGTTCCATTCATGTTCATGTGGTTAACAAAATCAAAACGTAACCGTGCAATCGGACGTGCTTCAGTAGTTCCAACTTTC
TTTGGTGTAAATGAACCAATCTTGTTTGGTGCACCACTTGTATTAAATCCAATCTTCTTCATTCCATTTATCTTTGCTCC
AATCGCAAACGTATGGATTTTCAAATTCTTTATTGAAACTCTTGGAATGAACTCATTCACTGCTAATCTACCATGGACAA
CTCCAGCTCCACTAGGTCTAGTTCTTGGAACTAACTTCCAAGTGCTATCATTCATTCTTGCTGCCCTTCTAATCGTGGTT
GACGTTGTCATTTACTATCCATTCCTTAAGGTCTATGATGAACAAATTCTTGAAGAAGAACGTTCAGGTAAGTCTAATGA
TGAATTGAAAGAAAAAGTTGCTGCAAACTTCAACACTGCAAAAGCGGATGCTATTCTTGAAAAAGCGGGTGTCGATGCAG
CACAAAATACCATCACTGAAGAAACAAATGTCCTCGTTCTCTGTGCAGGTGGAGGAACAAGTGGTCTCCTTGCAAATGCT
TTGAATAAGGCAGCAGCAGAATACAATGTCCCTGTGAAAGCAGCAGCAGGCGGCTATGGTGCTCACCGTGAAATGTTACC
AGAGTTTGATCTTGTTATCCTTGCCCCTCAAGTTGCTTCAAACTTTGAAGATATGAAAGCAGAAACAGATAAGCTCGGTA
TTAAACTAGCGAAAACAGAAGGCGCTCAATACATCAAATTAACTCGTGATGGAAAAGGTGCTCTTGCATTCGTACAAGCG
CAATTCGATTAA

Upstream 100 bases:

>100_bases
GTGTGACCATGATGCATGGCCAAGACCACTTGATGACAACTATCTTGTTAAAGGATTTGATGCACCATTTGATCGAACTC
TATAAGAGAGGAGTTAAATA

Downstream 100 bases:

>100_bases
GGCTAGAGACTCTGAAATAGTCTCCTATCGTTACGGAAATCGCTATGGCGAATTTCCTATTATTAATTCGTCGGTAAAAA
GATATCGTTTTTACCTCCTC

Product: PTS system, lactose-specific IIBC components

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MNKLIAFIEKGKPFFDKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALL
VAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTF
FGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVV
DVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQA
QFD

Sequences:

>Translated_563_residues
MNKLIAFIEKGKPFFDKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALL
VAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTF
FGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVV
DVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQA
QFD
>Mature_563_residues
MNKLIAFIEKGKPFFDKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALL
VAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTF
FGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVV
DVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQA
QFD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=460, Percent_Identity=25.6521739130435, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61007; Mature: 61007

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLIAFIEKGKPFFDKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSD
CCHHHHHHHCCCCHHHHHCCCEEEEEHHCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCHH
EVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLA
HHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNVTIRMPDEVPPNISQVFKDVI
CCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHH
PFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFM
CCCHHHHHHHHHHEECCCEEEHHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
WLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIET
HHHHCHHHCCCCCHHHCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVVDVVIYYPFLKVYDEQILEEE
HCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
HCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHHHHHH
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTE
HHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHCCHHHHHHHHHHHCEEEEECC
GAQYIKLTRDGKGALAFVQAQFD
CCEEEEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MNKLIAFIEKGKPFFDKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSD
CCHHHHHHHCCCCHHHHHCCCEEEEEHHCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCHH
EVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLA
HHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNVTIRMPDEVPPNISQVFKDVI
CCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHH
PFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFM
CCCHHHHHHHHHHEECCCEEEHHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
WLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIET
HHHHCHHHCCCCCHHHCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVVDVVIYYPFLKVYDEQILEEE
HCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
HCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHHHHHH
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTE
HHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHCCHHHHHHHHHHHCEEEEECC
GAQYIKLTRDGKGALAFVQAQFD
CCEEEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2125052 [H]