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The map label for this gene is mvaS [H]

Identifier: 116515383

GI number: 116515383

Start: 1560004

End: 1561200

Strand: Reverse

Name: mvaS [H]

Synonym: SPD_1537

Alternate gene names: 116515383

Gene position: 1561200-1560004 (Counterclockwise)

Preceding gene: 116515856

Following gene: 116516718

Centisome position: 76.3

GC content: 42.11

Gene sequence:

>1197_bases
ATGAATGATAAAACAGAGGTAAATATGACAATCGGTATTGATAAGATTGGTTTTGCGACCAGTCAATATGTCTTGAAATT
ACAAGACTTAGCAGAAGCGAGGGGAATTGACCCTGAAAAATTAAGTAAAGGACTCTTACTCAAGGAATTGAGTATTGCGC
CCCTAACTGAGGACATCGTGACCTTGGCGGCCAGTGCTAGTGACTCTATTTTAACTGAGCAAGAAAGACAAGAAGTTGAC
ATGGTCATTGTGGCTACCGAGTCAGGAATTGACCAGAGTAAGGCTGCGGCCGTCTTTGTGCATGGCTTGCTGGGCATCCA
GCCCTTTGCTCGTAGTTTCGAGATTAAAGAAGCCTGCTATGGGGCGACAGCTGCCCTCCATTATGCCAAATTGCATGTGG
AAAATTCTCCGGAGTCCAAGGTCTTGGTCATTGCCAGTGATATTGCCAAATATGGTATTGAAACTCCGGGAGAACCAACT
CAAGGTGCTGGAAGTGTAGCTATGTTGATTACACAAAATCCACGCATGATGGCCTTTAATAATGACAATGTAGCTCAGAC
CCGTGACATCATGGATTTCTGGCGACCAAATTACTCGACAACTCCTTATGTAAATGGTGTCTATTCTACCCAACAATACT
TGGATAGTTTGAAAACGACTTGGCTTGAATATCAAAAACGCTACCAGCTTACTTTGGATGATTTTGCGGCTGTTTGTTTC
CACTTGCCTTATCCTAAATTAGCGCTAAAAGGCTTGAAAAAAATCATGGATAAGAGCCTGCCTCAAGAGAAAAAAGACCT
CTTACAAAAGCATTTTGACCAGTCTATTCTCTACAGTCAAAAGGTGGGGAATATCTACACAGGTTCACTTTTCCTTGGAC
TTTTGTCTCTCTTGGAAAATACAGATAGCTTGAAAGCTGGGGATAAAATCGTCCTTTATAGTTACGGAAGTGGAGCTGTG
GCTGAGTTCTTCAGTGGTGAATTGGTTGAAGGATATGAAGCTTATTTGGATAAAGACCGCTTGAACAAGCTCAACCAACG
AACTGCCCTATCCGTTGCAGACTATGAAAAGGTCTTTTTTGAGGAAGTAAACTTGGATGAAACAAACTCTGCCCAGTTTG
CTGGCTATGAAAATCAAGATTTTGCCTTGGTTGAAATTCTTGACCACCAACGCCGTTATAGCAAGGTTGAAAAATAA

Upstream 100 bases:

>100_bases
TTTAAAATGATGACCAATAAGAGGCTTGATTTGGTGAATCAAGTCTTTTTTCTCTCTTTTACTGCCATTTGTGATAAAAT
AGTATAGAACGATTTTTTAC

Downstream 100 bases:

>100_bases
TGAAGATAAGTTGGAATGGATTTTCTAAAAAATCATACCAAGAGCGCCTCGAGCTGCTAAAAGCTCAGGCGCTCCTTAGT
CCTGAGAGACAAGCTAGTCT

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIVTLAASASDSILTEQERQEVD
MVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPT
QGAGSVAMLITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLENTDSLKAGDKIVLYSYGSGAV
AEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK

Sequences:

>Translated_398_residues
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIVTLAASASDSILTEQERQEVD
MVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPT
QGAGSVAMLITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLENTDSLKAGDKIVLYSYGSGAV
AEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK
>Mature_398_residues
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIVTLAASASDSILTEQERQEVD
MVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPT
QGAGSVAMLITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLENTDSLKAGDKIVLYSYGSGAV
AEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=449, Percent_Identity=27.1714922048998, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI148298764, Length=416, Percent_Identity=27.1634615384615, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI148298677, Length=416, Percent_Identity=27.1634615384615, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17560126, Length=371, Percent_Identity=25.3369272237197, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323509, Length=400, Percent_Identity=24.5, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24654139, Length=374, Percent_Identity=27.5401069518717, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654137, Length=374, Percent_Identity=27.5401069518717, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654133, Length=374, Percent_Identity=27.5401069518717, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654135, Length=374, Percent_Identity=27.5401069518717, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17933694, Length=374, Percent_Identity=27.5401069518717, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 44464; Mature: 44464

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIV
CCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHCCCCCCHHHHH
TLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACY
HHHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAMLITQNPRMMAFN
HHHHHHHEEEEEECCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEC
NDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLEN
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCC
TDSLKAGDKIVLYSYGSGAVAEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFF
CCCCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
EEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIV
CCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHCCCCCCHHHHH
TLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACY
HHHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAMLITQNPRMMAFN
HHHHHHHEEEEEECCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEC
NDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLEN
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCC
TDSLKAGDKIVLYSYGSGAVAEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFF
CCCCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
EEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]