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The map label for this gene is dinB [H]

Identifier: 116494323

GI number: 116494323

Start: 774614

End: 775717

Strand: Direct

Name: dinB [H]

Synonym: LSEI_0779

Alternate gene names: 116494323

Gene position: 774614-775717 (Clockwise)

Preceding gene: 116494322

Following gene: 116494324

Centisome position: 26.75

GC content: 48.46

Gene sequence:

>1104_bases
ATGGCGTTATTTGAATTACCATTACAAAACGATACTTCCCGGAAAATCATTCATCTGGATATGGACGCCTTTTATGCTTC
AATTGAGATGCGCGATAATCCGCAATTGCGTAGTAAAGCCTTGGTAATTGCCCGGGATCCGCGTACAACTGGCGGCAAAG
GTGTTGTGACAACGGCCAACTATGTCGCCAGACGTTATGGCGTTCATTCGGCAATGCCGGCCAACGAAGCCTTGCACCTT
GTGCCTCGCTCAAAACTGGTGTTTAAGACGCCGGATTTTCCAAAATATAAAGCAGTGTCCGCGCAAATTCACGCCTTGTT
TCATCAAGTGACCGATCTAATTGAACCAGTGGCGTTCGATGAAGCTTATCTTGATGTCACGGGCAACAAGATGTTCCCGA
GTACGATCGCCTTGGCATTGTGGCTGCAAGATCAAATTAGTCAGGCAACGCAGCTCACAAGTTCCATTGGCATTTCCTAC
AATAAATTTATCGCCAAGCAAGCTTCTGATTACAATAAACCGGTTGGTCGTACACTGGTTTTGCCAGAACAAGCCCTATT
ATTTTTGGATCGGCTGCCGATCAAGCAGTTTCGCGGGGTTGGTAAGAAAACGTTGCCGAAGTTAACCGACTTAGGTGTCA
CTGATGGCAAGTCTTTACGGGCCTTATCGCAAGATGATTTGCTCCGGATGTTTGGCAAAATGGGATTTGTGCTTTATCAA
CATGCTCGTGGCGTTGACAATCGTCCTGTTGCTGTTCGGACGGCGAAGTCAATTGGCAAAGAACGAACCTATGGCACGCC
TCTAACTGACGCCGAGGCGGTTCAGACACAGTTACACAATCTGGCAGGGATGGTCGTGACATCGTTGAATCAAAAAGCTA
TGCACGGCAAAACCGTGGTGCTTAAGGTGCGCGATGTGGACTTCATCACGCAAACAAAGCGGCTAACCCAAGATCACTAC
TTCGAAGGCGAACAAGCCATTTTCGATGCAGCGTGGCAGCTCTGGGGCAGTGTTGCCATGTCGAACTTGGCCATCCGGTT
ACTTGGGATTACCGTGACCGGGTTGGATCCGAAGCAGTATGAGAATTTGGACTTACCGTTGTGA

Upstream 100 bases:

>100_bases
TAGGTGGTCCGAGTAGAATCGGTCTTGATCGTTAAAGCTGAACGTGAACTAGCGCGACAGAAAGAATAACAGAACAGTTG
ATACGAGGAAGGGGAAGCTC

Downstream 100 bases:

>100_bases
AGACAGACAGCGAAGGGGTGCTTGCTGTAGGGGCTTTGGTAGCGGCATGCGCTACTTGAGCTAATTAATGCGGTTTAGCG
CACGTTTTTCCCATAACGCG

Product: nucleotidyltransferase/DNA polymerase for DNA repair

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MALFELPLQNDTSRKIIHLDMDAFYASIEMRDNPQLRSKALVIARDPRTTGGKGVVTTANYVARRYGVHSAMPANEALHL
VPRSKLVFKTPDFPKYKAVSAQIHALFHQVTDLIEPVAFDEAYLDVTGNKMFPSTIALALWLQDQISQATQLTSSIGISY
NKFIAKQASDYNKPVGRTLVLPEQALLFLDRLPIKQFRGVGKKTLPKLTDLGVTDGKSLRALSQDDLLRMFGKMGFVLYQ
HARGVDNRPVAVRTAKSIGKERTYGTPLTDAEAVQTQLHNLAGMVVTSLNQKAMHGKTVVLKVRDVDFITQTKRLTQDHY
FEGEQAIFDAAWQLWGSVAMSNLAIRLLGITVTGLDPKQYENLDLPL

Sequences:

>Translated_367_residues
MALFELPLQNDTSRKIIHLDMDAFYASIEMRDNPQLRSKALVIARDPRTTGGKGVVTTANYVARRYGVHSAMPANEALHL
VPRSKLVFKTPDFPKYKAVSAQIHALFHQVTDLIEPVAFDEAYLDVTGNKMFPSTIALALWLQDQISQATQLTSSIGISY
NKFIAKQASDYNKPVGRTLVLPEQALLFLDRLPIKQFRGVGKKTLPKLTDLGVTDGKSLRALSQDDLLRMFGKMGFVLYQ
HARGVDNRPVAVRTAKSIGKERTYGTPLTDAEAVQTQLHNLAGMVVTSLNQKAMHGKTVVLKVRDVDFITQTKRLTQDHY
FEGEQAIFDAAWQLWGSVAMSNLAIRLLGITVTGLDPKQYENLDLPL
>Mature_366_residues
ALFELPLQNDTSRKIIHLDMDAFYASIEMRDNPQLRSKALVIARDPRTTGGKGVVTTANYVARRYGVHSAMPANEALHLV
PRSKLVFKTPDFPKYKAVSAQIHALFHQVTDLIEPVAFDEAYLDVTGNKMFPSTIALALWLQDQISQATQLTSSIGISYN
KFIAKQASDYNKPVGRTLVLPEQALLFLDRLPIKQFRGVGKKTLPKLTDLGVTDGKSLRALSQDDLLRMFGKMGFVLYQH
ARGVDNRPVAVRTAKSIGKERTYGTPLTDAEAVQTQLHNLAGMVVTSLNQKAMHGKTVVLKVRDVDFITQTKRLTQDHYF
EGEQAIFDAAWQLWGSVAMSNLAIRLLGITVTGLDPKQYENLDLPL

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=342, Percent_Identity=26.0233918128655, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI7706681, Length=343, Percent_Identity=25.9475218658892, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI154350220, Length=337, Percent_Identity=26.1127596439169, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI7705344, Length=124, Percent_Identity=39.5161290322581, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1786425, Length=363, Percent_Identity=35.8126721763085, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1787432, Length=292, Percent_Identity=22.2602739726027, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17537959, Length=328, Percent_Identity=25.609756097561, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI193205700, Length=129, Percent_Identity=35.6589147286822, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355641, Length=260, Percent_Identity=31.5384615384615, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644984, Length=260, Percent_Identity=31.5384615384615, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19923006, Length=352, Percent_Identity=23.5795454545455, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24668444, Length=176, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40867; Mature: 40736

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFELPLQNDTSRKIIHLDMDAFYASIEMRDNPQLRSKALVIARDPRTTGGKGVVTTAN
CCEEECCCCCCCCCEEEEEEHHHEEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEHHH
YVARRYGVHSAMPANEALHLVPRSKLVFKTPDFPKYKAVSAQIHALFHQVTDLIEPVAFD
HHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
EAYLDVTGNKMFPSTIALALWLQDQISQATQLTSSIGISYNKFIAKQASDYNKPVGRTLV
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEE
LPEQALLFLDRLPIKQFRGVGKKTLPKLTDLGVTDGKSLRALSQDDLLRMFGKMGFVLYQ
CCHHHHHHHHCCCHHHHHCCCHHHCCHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
HARGVDNRPVAVRTAKSIGKERTYGTPLTDAEAVQTQLHNLAGMVVTSLNQKAMHGKTVV
HHCCCCCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCEEE
LKVRDVDFITQTKRLTQDHYFEGEQAIFDAAWQLWGSVAMSNLAIRLLGITVTGLDPKQY
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHH
ENLDLPL
CCCCCCC
>Mature Secondary Structure 
ALFELPLQNDTSRKIIHLDMDAFYASIEMRDNPQLRSKALVIARDPRTTGGKGVVTTAN
CEEECCCCCCCCCEEEEEEHHHEEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEHHH
YVARRYGVHSAMPANEALHLVPRSKLVFKTPDFPKYKAVSAQIHALFHQVTDLIEPVAFD
HHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
EAYLDVTGNKMFPSTIALALWLQDQISQATQLTSSIGISYNKFIAKQASDYNKPVGRTLV
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEE
LPEQALLFLDRLPIKQFRGVGKKTLPKLTDLGVTDGKSLRALSQDDLLRMFGKMGFVLYQ
CCHHHHHHHHCCCHHHHHCCCHHHCCHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
HARGVDNRPVAVRTAKSIGKERTYGTPLTDAEAVQTQLHNLAGMVVTSLNQKAMHGKTVV
HHCCCCCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCEEE
LKVRDVDFITQTKRLTQDHYFEGEQAIFDAAWQLWGSVAMSNLAIRLLGITVTGLDPKQY
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHH
ENLDLPL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]