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The map label for this gene is ciaR [H]

Identifier: 116494259

GI number: 116494259

Start: 714387

End: 715082

Strand: Direct

Name: ciaR [H]

Synonym: LSEI_0711

Alternate gene names: 116494259

Gene position: 714387-715082 (Clockwise)

Preceding gene: 116494253

Following gene: 116494260

Centisome position: 24.67

GC content: 42.39

Gene sequence:

>696_bases
ATGACAGTTAAACCGAGAATTCTTGTGATCGAAGACGATCCGGATTTAAACGAGAGTATTACTGATTTTATCCGCGCATT
TGCCGACCCAGAAGCCGCCTTAAACGGTGCTGATGGTCTGTATTTGGCCGAGGAAGGTATTTTTGATGCCATTATTTTGG
ACATTATGTTGCCAGAATTAAATGGTTTTGACGTACTCAAGAAGTTGCGGGCAAAGCAAATTAAAACGCCTGTATTGATT
TTGACAGCCAAAGATGCGCTGACCGATAAATTGCACGGGTTTGAACTTGGCGGAGATGATTATCTGACGAAGCCGTTTCA
TCGTGAAGAACTCATTATGCGTTTGAAGGCGTTGTTAAAGCGAACCGGTCACTTCTTTGACGATGACGGCTTAACTGCTG
GTCCTTTTGTGGTACATGTGACGAGTCATGAGGTGCTCATCAATGATCAACCAGTGGCCTTGAACGGTAAAGAATTCGAT
CTGTTGGTATATCTATTACAAAATCAGAAAACAATTATCACCAAAGATCAGATTTTTGATCGTTTGTGGGGATTTGATTC
TGAAACAGCTGTCAGCGTGGTGGAGGTTTACATGTCCAACTTGCGCAAAAAATTAAAACCGCTAAAAGTTGAGCATTGGA
TCAAAACGATTCGCAACGTTGGCTATATTTTTCAGGAGCCGGAGGGCAGTGAATGA

Upstream 100 bases:

>100_bases
CTTAAAGTCGGGCATTCTATTTCTTAAGATGGCTTTATGGTTATCATGATATGGTCAGACCAGCAGATATAAGGTTACCA
ACAAATAAGTGGGGCAGAAC

Downstream 100 bases:

>100_bases
ACGCAGAAAAAGGTATTAAACATCAGCAACGTCGCTTATTCATCGGGCAACTGTTGAGCTTTGCGGGCTTGTTTGTGGTA
TTAGGTATCATTGTTTTTTT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTVKPRILVIEDDPDLNESITDFIRAFADPEAALNGADGLYLAEEGIFDAIILDIMLPELNGFDVLKKLRAKQIKTPVLI
LTAKDALTDKLHGFELGGDDYLTKPFHREELIMRLKALLKRTGHFFDDDGLTAGPFVVHVTSHEVLINDQPVALNGKEFD
LLVYLLQNQKTIITKDQIFDRLWGFDSETAVSVVEVYMSNLRKKLKPLKVEHWIKTIRNVGYIFQEPEGSE

Sequences:

>Translated_231_residues
MTVKPRILVIEDDPDLNESITDFIRAFADPEAALNGADGLYLAEEGIFDAIILDIMLPELNGFDVLKKLRAKQIKTPVLI
LTAKDALTDKLHGFELGGDDYLTKPFHREELIMRLKALLKRTGHFFDDDGLTAGPFVVHVTSHEVLINDQPVALNGKEFD
LLVYLLQNQKTIITKDQIFDRLWGFDSETAVSVVEVYMSNLRKKLKPLKVEHWIKTIRNVGYIFQEPEGSE
>Mature_230_residues
TVKPRILVIEDDPDLNESITDFIRAFADPEAALNGADGLYLAEEGIFDAIILDIMLPELNGFDVLKKLRAKQIKTPVLIL
TAKDALTDKLHGFELGGDDYLTKPFHREELIMRLKALLKRTGHFFDDDGLTAGPFVVHVTSHEVLINDQPVALNGKEFDL
LVYLLQNQKTIITKDQIFDRLWGFDSETAVSVVEVYMSNLRKKLKPLKVEHWIKTIRNVGYIFQEPEGSE

Specific function: Member of the two-component regulatory system CiaH/CiaR. Involved in early steps of competence regulation and in penicillin susceptibility [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=32.4675324675325, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=34.6666666666667, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=35.9090909090909, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=31.5315315315315, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1790552, Length=227, Percent_Identity=31.7180616740088, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=29.7297297297297, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI2367329, Length=179, Percent_Identity=35.195530726257, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789809, Length=240, Percent_Identity=27.9166666666667, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790860, Length=182, Percent_Identity=32.967032967033, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=28.125, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=30.9012875536481, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787229, Length=209, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=26.9565217391304, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI145693140, Length=200, Percent_Identity=28.5, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=132, Percent_Identity=28.7878787878788, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26207; Mature: 26075

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKPRILVIEDDPDLNESITDFIRAFADPEAALNGADGLYLAEEGIFDAIILDIMLPEL
CCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCEEEECCCHHHHHHHHHHCCCC
NGFDVLKKLRAKQIKTPVLILTAKDALTDKLHGFELGGDDYLTKPFHREELIMRLKALLK
CCHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHH
RTGHFFDDDGLTAGPFVVHVTSHEVLINDQPVALNGKEFDLLVYLLQNQKTIITKDQIFD
HHCCEECCCCCCCCCEEEEEECCEEEECCCCEEECCCCCEEEEEEECCCCEEEEHHHHHH
RLWGFDSETAVSVVEVYMSNLRKKLKPLKVEHWIKTIRNVGYIFQEPEGSE
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure 
TVKPRILVIEDDPDLNESITDFIRAFADPEAALNGADGLYLAEEGIFDAIILDIMLPEL
CCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCEEEECCCHHHHHHHHHHCCCC
NGFDVLKKLRAKQIKTPVLILTAKDALTDKLHGFELGGDDYLTKPFHREELIMRLKALLK
CCHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHH
RTGHFFDDDGLTAGPFVVHVTSHEVLINDQPVALNGKEFDLLVYLLQNQKTIITKDQIFD
HHCCEECCCCCCCCCEEEEEECCEEEECCCCEEECCCCCEEEEEEECCCCEEEEHHHHHH
RLWGFDSETAVSVVEVYMSNLRKKLKPLKVEHWIKTIRNVGYIFQEPEGSE
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]