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The map label for this gene is galR [H]

Identifier: 116494215

GI number: 116494215

Start: 670890

End: 671903

Strand: Direct

Name: galR [H]

Synonym: LSEI_0667

Alternate gene names: 116494215

Gene position: 670890-671903 (Clockwise)

Preceding gene: 116494214

Following gene: 116494216

Centisome position: 23.17

GC content: 52.07

Gene sequence:

>1014_bases
ATGGAAACGGAGAAAACGATGACCACGATTACCGATATTGCGAAGGCGGCAGGGGTTTCAATTGCGACTGTCTCGCGTAT
TTTAAATTATGATACGACGTTGGCTGTCACGCCCGAAACGCGCCAACGCGTGTTGAAAACAGCCGAGAAGCTGGCGTACA
AACCACGTCGACGTAAACGTGTGCCTACGCAAACCACCGTTGCCTTGATTCAGTGGCGCAGCGAGCAAGAAGAGCTGAAT
GATCTGTATTACTTGCAGATTCAGTATGCCATTGAGGCCCGGGCGGCTTCGGCTGGGTACCATTTGCAAAATCTGCATTT
GGAGCAGTTGGGAGCTGAGACGACACAAAACGTCCAAGGTGTGATTGCGTTGGGTAAGTACGACGCTGGCGAAGTGGCAA
CGATCAAAGCCTTGGCGCAACCAGTTGTTTTCGTGGATCAAAATATGTTGGCATTTGACTGCGACTCGGTCACCAGCGAT
TATGCCGGTCCTATCAAGCAAATTATTCATCATTTTCAATCGGTAGGGATCAACGACATCGGGTTTCTCGGTGGCGTTGA
AACGAGTCGCAGCGGACGCGAGCAGTTTCAGGATGTTCGGACAGCCGCATTTGATCGGGTGATGCATGCAGAAAACTTGA
TGCAGCCAGCGTTTCGTTTTATTGGCGCGTTCACGCCTGATTCAGGTTACGAGCTGATGAAACAAGCGATCACCAAACTC
GGGGACACCTTACCCCACGGTTTCATTGTTGCCAACGACACGATGGCAGTCGGTGCGTTGCGCGCTTTGAACGAGGCTAA
GATCGAGGTGCCGCAACGCGTCTCTGTGATCAGCTTCAACGATGTGGCGGTCGCCAAGTTCACGACCCCGCCACTTTCTA
CGGTGCATGCTGCCACTGACCAAATGGGCCAAACCGCGGTCGAACTATTGCAGGCACGCATCAAAGATCCGAAACGCGTA
CCACGTCAGGTTAACTTCAAAAGTGAGCTTATTATGCGAGCCAGCAGCTTGTAG

Upstream 100 bases:

>100_bases
CTGGTGTGTTCAAACGCGATGAAGTTGGTCAAAAAGCATTTGCTCGCTTCTGTCGGGCACTGTAGCAACAGGCTATGTTG
CTGAAGCCGGCAAGGAGTCA

Downstream 100 bases:

>100_bases
GAACGGTATCATAACGTGTTCCCCCGCGAAGAAACCTGTCCATAATGACCTCAAGTGCAATGACCAAAAGCGGGTCATCA
TACTTGAGGTCGGCTGATGC

Product: transcription regulator of beta-galactosidase gene

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
METEKTMTTITDIAKAAGVSIATVSRILNYDTTLAVTPETRQRVLKTAEKLAYKPRRRKRVPTQTTVALIQWRSEQEELN
DLYYLQIQYAIEARAASAGYHLQNLHLEQLGAETTQNVQGVIALGKYDAGEVATIKALAQPVVFVDQNMLAFDCDSVTSD
YAGPIKQIIHHFQSVGINDIGFLGGVETSRSGREQFQDVRTAAFDRVMHAENLMQPAFRFIGAFTPDSGYELMKQAITKL
GDTLPHGFIVANDTMAVGALRALNEAKIEVPQRVSVISFNDVAVAKFTTPPLSTVHAATDQMGQTAVELLQARIKDPKRV
PRQVNFKSELIMRASSL

Sequences:

>Translated_337_residues
METEKTMTTITDIAKAAGVSIATVSRILNYDTTLAVTPETRQRVLKTAEKLAYKPRRRKRVPTQTTVALIQWRSEQEELN
DLYYLQIQYAIEARAASAGYHLQNLHLEQLGAETTQNVQGVIALGKYDAGEVATIKALAQPVVFVDQNMLAFDCDSVTSD
YAGPIKQIIHHFQSVGINDIGFLGGVETSRSGREQFQDVRTAAFDRVMHAENLMQPAFRFIGAFTPDSGYELMKQAITKL
GDTLPHGFIVANDTMAVGALRALNEAKIEVPQRVSVISFNDVAVAKFTTPPLSTVHAATDQMGQTAVELLQARIKDPKRV
PRQVNFKSELIMRASSL
>Mature_337_residues
METEKTMTTITDIAKAAGVSIATVSRILNYDTTLAVTPETRQRVLKTAEKLAYKPRRRKRVPTQTTVALIQWRSEQEELN
DLYYLQIQYAIEARAASAGYHLQNLHLEQLGAETTQNVQGVIALGKYDAGEVATIKALAQPVVFVDQNMLAFDCDSVTSD
YAGPIKQIIHHFQSVGINDIGFLGGVETSRSGREQFQDVRTAAFDRVMHAENLMQPAFRFIGAFTPDSGYELMKQAITKL
GDTLPHGFIVANDTMAVGALRALNEAKIEVPQRVSVISFNDVAVAKFTTPPLSTVHAATDQMGQTAVELLQARIKDPKRV
PRQVNFKSELIMRASSL

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=337, Percent_Identity=36.2017804154303, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1787948, Length=344, Percent_Identity=28.7790697674419, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789068, Length=341, Percent_Identity=26.9794721407625, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1790194, Length=348, Percent_Identity=25.5747126436782, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786540, Length=315, Percent_Identity=27.6190476190476, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788474, Length=318, Percent_Identity=27.6729559748428, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787580, Length=351, Percent_Identity=24.2165242165242, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790369, Length=351, Percent_Identity=24.7863247863248, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789202, Length=352, Percent_Identity=26.7045454545455, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=25.1533742331288, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=26.5861027190332, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00356 LacI [H]

EC number: NA

Molecular weight: Translated: 37211; Mature: 37211

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METEKTMTTITDIAKAAGVSIATVSRILNYDTTLAVTPETRQRVLKTAEKLAYKPRRRKR
CCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCHHHCC
VPTQTTVALIQWRSEQEELNDLYYLQIQYAIEARAASAGYHLQNLHLEQLGAETTQNVQG
CCCHHHHHHHHHCCHHHHCCCEEEEEEEEEEHHHHHHCCCHHHHCCHHHHCCHHHCCCCE
VIALGKYDAGEVATIKALAQPVVFVDQNMLAFDCDSVTSDYAGPIKQIIHHFQSVGINDI
EEEECCCCCCCHHHHHHHHCCEEEECCCEEEEECHHHCCCCCHHHHHHHHHHHHCCCCCH
GFLGGVETSRSGREQFQDVRTAAFDRVMHAENLMQPAFRFIGAFTPDSGYELMKQAITKL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GDTLPHGFIVANDTMAVGALRALNEAKIEVPQRVSVISFNDVAVAKFTTPPLSTVHAATD
HCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHH
QMGQTAVELLQARIKDPKRVPRQVNFKSELIMRASSL
HHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
METEKTMTTITDIAKAAGVSIATVSRILNYDTTLAVTPETRQRVLKTAEKLAYKPRRRKR
CCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCHHHCC
VPTQTTVALIQWRSEQEELNDLYYLQIQYAIEARAASAGYHLQNLHLEQLGAETTQNVQG
CCCHHHHHHHHHCCHHHHCCCEEEEEEEEEEHHHHHHCCCHHHHCCHHHHCCHHHCCCCE
VIALGKYDAGEVATIKALAQPVVFVDQNMLAFDCDSVTSDYAGPIKQIIHHFQSVGINDI
EEEECCCCCCCHHHHHHHHCCEEEECCCEEEEECHHHCCCCCHHHHHHHHHHHHCCCCCH
GFLGGVETSRSGREQFQDVRTAAFDRVMHAENLMQPAFRFIGAFTPDSGYELMKQAITKL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GDTLPHGFIVANDTMAVGALRALNEAKIEVPQRVSVISFNDVAVAKFTTPPLSTVHAATD
HCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHH
QMGQTAVELLQARIKDPKRVPRQVNFKSELIMRASSL
HHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9603808 [H]