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The map label for this gene is nisX1 [H]

Identifier: 116494081

GI number: 116494081

Start: 522436

End: 523266

Strand: Direct

Name: nisX1 [H]

Synonym: LSEI_0524

Alternate gene names: 116494081

Gene position: 522436-523266 (Clockwise)

Preceding gene: 116494080

Following gene: 116494082

Centisome position: 18.04

GC content: 45.61

Gene sequence:

>831_bases
ATGATTCAAGATCAATTGAGCCGGGGGCACCGCATCACAGTTATTCTCCGAGCGTTACGGATCCCTTCGAGTACCTACTA
TGACTGGTTAAAGTGGCATCCTAAGTCACGAAATCGCCGCCGAATTAAGCTTAAAGAGCTGGTTCAGGTTCTTTGGCAAC
GTCGAAAATTTTACGGATACGTGCGGATTGCTAAACGCATTCGTAAATTACTTAAATGCCGCTTAAGTGACCGCACGATT
TGGAAAGTCATGCGTGAATTGGGGATTCAATCGACAATGTATCGTAAACGCTCTAAAAAGCCTACCACAACCACTGACAT
GCCACAAAAACCTAATTTAATGCGACACCTAGCTGACTTGTCTGAGGTCGTGACCACCGATATCACCTATATTCAACTGA
TCAACCAAAAATGGGTTTACCTTGCAACAGCGTATGATCCAAAAGCGAGAAAAGTCTTAGCTTGGCAAGTGGGCCAACAG
ATGACACAAGACTTCGCGGTCGCACCGATTCAAGCGTTAATTCACCAAGGTTATACTTTTAAGATGGTGCATAGCGATAT
GGGTAGTCAATATACCAGTCGTTTATTTGAGACAACATTGACAGATGCGCACCTGCGTCACTCATATTCGCGCAAAGGCA
AACCGGCTGATAACGGGCGGATCGAAGCCTACCATTCGCTATTGAAACGAGAATGGGTCCGGCTGGAGCAGATCAACTAT
GAATCAATTCTAGACGTCACTGAATCCATTGCTCGTTACAACACCTTCTACAATCATGATCGCGAGACCAACGGTCGCGG
TGCTTGCAAAAGAAAGTCAGCAGCTGCATAA

Upstream 100 bases:

>100_bases
AAGAAAATCGCCGTTTGAGGGAAGAACTCGATATTTTAAAACGAGCGGCGGTGTTGCTGGCAAAGCGTTGATTCATAGCG
GCCGAAAGGCCGCTCTTTTT

Downstream 100 bases:

>100_bases
GTAACCTTTCAATAGGCGCACAGCCCTGGGGAAAGACCAGATGCCAGGGCTTGTTTGTAGTCTCTCTTGCACGTTAAACA
AGATTAAACGCTGATAAAAG

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIQDQLSRGHRITVILRALRIPSSTYYDWLKWHPKSRNRRRIKLKELVQVLWQRRKFYGYVRIAKRIRKLLKCRLSDRTI
WKVMRELGIQSTMYRKRSKKPTTTTDMPQKPNLMRHLADLSEVVTTDITYIQLINQKWVYLATAYDPKARKVLAWQVGQQ
MTQDFAVAPIQALIHQGYTFKMVHSDMGSQYTSRLFETTLTDAHLRHSYSRKGKPADNGRIEAYHSLLKREWVRLEQINY
ESILDVTESIARYNTFYNHDRETNGRGACKRKSAAA

Sequences:

>Translated_276_residues
MIQDQLSRGHRITVILRALRIPSSTYYDWLKWHPKSRNRRRIKLKELVQVLWQRRKFYGYVRIAKRIRKLLKCRLSDRTI
WKVMRELGIQSTMYRKRSKKPTTTTDMPQKPNLMRHLADLSEVVTTDITYIQLINQKWVYLATAYDPKARKVLAWQVGQQ
MTQDFAVAPIQALIHQGYTFKMVHSDMGSQYTSRLFETTLTDAHLRHSYSRKGKPADNGRIEAYHSLLKREWVRLEQINY
ESILDVTESIARYNTFYNHDRETNGRGACKRKSAAA
>Mature_276_residues
MIQDQLSRGHRITVILRALRIPSSTYYDWLKWHPKSRNRRRIKLKELVQVLWQRRKFYGYVRIAKRIRKLLKCRLSDRTI
WKVMRELGIQSTMYRKRSKKPTTTTDMPQKPNLMRHLADLSEVVTTDITYIQLINQKWVYLATAYDPKARKVLAWQVGQQ
MTQDFAVAPIQALIHQGYTFKMVHSDMGSQYTSRLFETTLTDAHLRHSYSRKGKPADNGRIEAYHSLLKREWVRLEQINY
ESILDVTESIARYNTFYNHDRETNGRGACKRKSAAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=264, Percent_Identity=26.5151515151515, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788405, Length=244, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787263, Length=244, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786753, Length=244, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786570, Length=244, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786490, Length=244, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789981, Length=270, Percent_Identity=22.962962962963, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32735; Mature: 32735

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQDQLSRGHRITVILRALRIPSSTYYDWLKWHPKSRNRRRIKLKELVQVLWQRRKFYGY
CCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VRIAKRIRKLLKCRLSDRTIWKVMRELGIQSTMYRKRSKKPTTTTDMPQKPNLMRHLADL
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
SEVVTTDITYIQLINQKWVYLATAYDPKARKVLAWQVGQQMTQDFAVAPIQALIHQGYTF
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
KMVHSDMGSQYTSRLFETTLTDAHLRHSYSRKGKPADNGRIEAYHSLLKREWVRLEQINY
EEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCH
ESILDVTESIARYNTFYNHDRETNGRGACKRKSAAA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIQDQLSRGHRITVILRALRIPSSTYYDWLKWHPKSRNRRRIKLKELVQVLWQRRKFYGY
CCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VRIAKRIRKLLKCRLSDRTIWKVMRELGIQSTMYRKRSKKPTTTTDMPQKPNLMRHLADL
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
SEVVTTDITYIQLINQKWVYLATAYDPKARKVLAWQVGQQMTQDFAVAPIQALIHQGYTF
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
KMVHSDMGSQYTSRLFETTLTDAHLRHSYSRKGKPADNGRIEAYHSLLKREWVRLEQINY
EEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCH
ESILDVTESIARYNTFYNHDRETNGRGACKRKSAAA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]