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The map label for this gene is ydgK [H]

Identifier: 116493459

GI number: 116493459

Start: 1707292

End: 1708509

Strand: Reverse

Name: ydgK [H]

Synonym: PEPE_1731

Alternate gene names: 116493459

Gene position: 1708509-1707292 (Counterclockwise)

Preceding gene: 116493461

Following gene: 116493458

Centisome position: 93.24

GC content: 39.16

Gene sequence:

>1218_bases
ATGAATGAAAAGATATCAACTGTAAGAAAATGGTGGCTTGTATTTTTTATGGGAGTTGTTAGTGCGACAGGTCCTTTATC
AATGGATATGTATTTGCCGGGTCTACCTGAAATGCAACGAGCTTTTCACTCATCAGCTTCTACCTTACAATTGAGCATCA
CATTTTGTTTGATCGGGTTAGCGGTTGGACAGTTAATTGTAGGCCCTATTAGTGATCGGATTGGACGACGAGTTCCATTA
GTAGTCGGATTTGGATTATATGCGGTGACCTCATTGATGCTAATTTTTACTACCAATATCTATCTGTTTATTGGAATTCG
CTTAATTCAAGGGCTAGCGGGCTCAACCGGGCAAGTGCTCTCAAGAGCGGTGGCACGTGATTTGTTTAATGGACATGAAT
TAACTAAATTTTATGCAATGTTAATGGCGGTAAATGGTGCTTTTCCAATTATTGCGCCAATTATTGGAGGAGCATTACTT
TCTATTGTCCAATGGCAAGGGATTTTTGTACTATTATTTGTGATCGGAATTTTAGTGATGGTGGGGGTATTATGTACCTT
ACCCGAAACGTTGAAGGTAACCAACAATGAAGTCTTGACGCTTGGTCAAACTTTCAAAGGGTTGGGAGCAATGTTTGGTA
AACGACAATTTATTTTAATCGCTTTAACGCAAGGGCTGATTTTTGGTGCATTATTTAGCTATATTTCTGCTTCTTCATTT
GTATTTCAAACTTATTTCCACATGACAGTAGGGCAATTTAGTCTATTATATGCAATTAACGGACTGGGAATTATTCTTGG
TAACAATCTTCCAGGCTACCTACCAGAGCAAATCAAGGATAGCGATATCTTAAATGGCGGGTTAATTGGTGGCATGTTTG
CGGGAGTATTATTAGTTGGGAGCTTACTATTACGTCCAATGGCGATCTTAGTAATTATTCCCCTGTTTATTGTGGTCTTT
TGCGTGGGAGTTGTTAATACGGTGGCAACATCCATGGCTATGAGTCTAGGTGGAAGAACAAATGCTGGAGCGGCTTCAGC
CGTCTTAGGTTTATTAATGAATATTATTGGCGCTTTAGCTTCTCCATTAGCCGGAATAATGGGAACCCAGAGTTACGCTC
CTTTAGCTTGGTTAATCTTTGGTTTTGAATTTTTAGGATTGATTTTATTTCAATTGACCCGTTTTGAGGCGAAAAGTAGC
AATTATGTTGATAGGTAG

Upstream 100 bases:

>100_bases
ACAATTCTAACATATTAAGGATACCAAAATTTGATGTGTGATTTTGAACTAATTTTCGTTACACTTGAATTTGAAGTTTT
AAAAAAGGAGATCTGAGGTT

Downstream 100 bases:

>100_bases
GTTTCTATTTAACTGAAATCGTACTTCAATTGTTACAAATTATGTAGTAAAATTAAGTTCAAAGTGGGTGAAAAAATGGC
AAAAGATATTATAGCAGATG

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNEKISTVRKWWLVFFMGVVSATGPLSMDMYLPGLPEMQRAFHSSASTLQLSITFCLIGLAVGQLIVGPISDRIGRRVPL
VVGFGLYAVTSLMLIFTTNIYLFIGIRLIQGLAGSTGQVLSRAVARDLFNGHELTKFYAMLMAVNGAFPIIAPIIGGALL
SIVQWQGIFVLLFVIGILVMVGVLCTLPETLKVTNNEVLTLGQTFKGLGAMFGKRQFILIALTQGLIFGALFSYISASSF
VFQTYFHMTVGQFSLLYAINGLGIILGNNLPGYLPEQIKDSDILNGGLIGGMFAGVLLVGSLLLRPMAILVIIPLFIVVF
CVGVVNTVATSMAMSLGGRTNAGAASAVLGLLMNIIGALASPLAGIMGTQSYAPLAWLIFGFEFLGLILFQLTRFEAKSS
NYVDR

Sequences:

>Translated_405_residues
MNEKISTVRKWWLVFFMGVVSATGPLSMDMYLPGLPEMQRAFHSSASTLQLSITFCLIGLAVGQLIVGPISDRIGRRVPL
VVGFGLYAVTSLMLIFTTNIYLFIGIRLIQGLAGSTGQVLSRAVARDLFNGHELTKFYAMLMAVNGAFPIIAPIIGGALL
SIVQWQGIFVLLFVIGILVMVGVLCTLPETLKVTNNEVLTLGQTFKGLGAMFGKRQFILIALTQGLIFGALFSYISASSF
VFQTYFHMTVGQFSLLYAINGLGIILGNNLPGYLPEQIKDSDILNGGLIGGMFAGVLLVGSLLLRPMAILVIIPLFIVVF
CVGVVNTVATSMAMSLGGRTNAGAASAVLGLLMNIIGALASPLAGIMGTQSYAPLAWLIFGFEFLGLILFQLTRFEAKSS
NYVDR
>Mature_405_residues
MNEKISTVRKWWLVFFMGVVSATGPLSMDMYLPGLPEMQRAFHSSASTLQLSITFCLIGLAVGQLIVGPISDRIGRRVPL
VVGFGLYAVTSLMLIFTTNIYLFIGIRLIQGLAGSTGQVLSRAVARDLFNGHELTKFYAMLMAVNGAFPIIAPIIGGALL
SIVQWQGIFVLLFVIGILVMVGVLCTLPETLKVTNNEVLTLGQTFKGLGAMFGKRQFILIALTQGLIFGALFSYISASSF
VFQTYFHMTVGQFSLLYAINGLGIILGNNLPGYLPEQIKDSDILNGGLIGGMFAGVLLVGSLLLRPMAILVIIPLFIVVF
CVGVVNTVATSMAMSLGGRTNAGAASAVLGLLMNIIGALASPLAGIMGTQSYAPLAWLIFGFEFLGLILFQLTRFEAKSS
NYVDR

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=371, Percent_Identity=28.8409703504043, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI48994889, Length=229, Percent_Identity=30.1310043668122, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790794, Length=229, Percent_Identity=27.0742358078603, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=28.9156626506024, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787065, Length=228, Percent_Identity=25.8771929824561, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082312, Length=186, Percent_Identity=28.494623655914, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319657, Length=171, Percent_Identity=28.0701754385965, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43404; Mature: 43404

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]