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The map label for this gene is rluB [H]

Identifier: 116492846

GI number: 116492846

Start: 1080893

End: 1081618

Strand: Reverse

Name: rluB [H]

Synonym: PEPE_1084

Alternate gene names: 116492846

Gene position: 1081618-1080893 (Counterclockwise)

Preceding gene: 116492847

Following gene: 116492845

Centisome position: 59.03

GC content: 35.67

Gene sequence:

>726_bases
ATGGAAGAAGAAAGATTACAAAAAGTAATGGCTGAAGCAGGAGTTGCTTCAAGACGAAAGTCAGAAAAATTAATTACTGA
AGGCCATGTACGTGTTAATGGTAAGGTGATTAAACAGCTAGGTACAAAAGTAACTGCCCACGATAAGATTGAAGTTGATG
GTGTTCCTCTTCAACGAGAAAAGATGGTTTATTTCCTATTAAATAAACCTCGAGGGGTTATTACATCCGTTAGTGATGAT
AAAAAGCGTAAAACGGTCATGGACTTTTTCCCTGACGTAGTTGAACGTATTTATCCAGTGGGGCGTCTAGACTATGATAC
TTCAGGTGCCGTTTTAATGACCAATGATGGAACTTTAGCAAATGGTTTAACTCACCCCAAATTTAAGTTTGAAAAAACAT
ATACCGCAAAAGTTAAAGGAATTGTTAATAGTTTAGATTTAAAGAAGCTAGAAAATGGATTGGTAATTGATCATAAAAAA
ACAGCTAAAGCTAAAGCACACTTAATTAGCACGGATGATGAAAAGAAAACTTCGATTGTGAGCCTCACTATCCACGAAGG
GCGTAATCATCAAGTTAAAAATATGTTTATGGCAGTTGGTCACCCAGTCCAAAAATTACGCCGTGATAGTTACTCATTTT
TAAATATTGATGGAGTGCAATCTGGAAAATGGCGTGCTTTGAAGGTTAATGAAATTAGTCGTCTAAAACGTCAAATTGAA
AAATGA

Upstream 100 bases:

>100_bases
TAAACTTGAAAAAGTTGGAGAAGATTCAGAATTAGACGATAGCGATTTATTTTTACAAGAGTTTGAATCAAAAATGAATT
TAAATAATAAGGAGAAATAA

Downstream 100 bases:

>100_bases
TTCCAGAAGATGTTTTAGCTTTTTTTGATCTTAAGCAGGGGCGCCGTCCGAAGGCAATTTTGAATGTATTGGTTGGTAAA
ATGACGGTTTCGGCCTTATT

Product: ribosomal large subunit pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MEEERLQKVMAEAGVASRRKSEKLITEGHVRVNGKVIKQLGTKVTAHDKIEVDGVPLQREKMVYFLLNKPRGVITSVSDD
KKRKTVMDFFPDVVERIYPVGRLDYDTSGAVLMTNDGTLANGLTHPKFKFEKTYTAKVKGIVNSLDLKKLENGLVIDHKK
TAKAKAHLISTDDEKKTSIVSLTIHEGRNHQVKNMFMAVGHPVQKLRRDSYSFLNIDGVQSGKWRALKVNEISRLKRQIE
K

Sequences:

>Translated_241_residues
MEEERLQKVMAEAGVASRRKSEKLITEGHVRVNGKVIKQLGTKVTAHDKIEVDGVPLQREKMVYFLLNKPRGVITSVSDD
KKRKTVMDFFPDVVERIYPVGRLDYDTSGAVLMTNDGTLANGLTHPKFKFEKTYTAKVKGIVNSLDLKKLENGLVIDHKK
TAKAKAHLISTDDEKKTSIVSLTIHEGRNHQVKNMFMAVGHPVQKLRRDSYSFLNIDGVQSGKWRALKVNEISRLKRQIE
K
>Mature_241_residues
MEEERLQKVMAEAGVASRRKSEKLITEGHVRVNGKVIKQLGTKVTAHDKIEVDGVPLQREKMVYFLLNKPRGVITSVSDD
KKRKTVMDFFPDVVERIYPVGRLDYDTSGAVLMTNDGTLANGLTHPKFKFEKTYTAKVKGIVNSLDLKKLENGLVIDHKK
TAKAKAHLISTDDEKKTSIVSLTIHEGRNHQVKNMFMAVGHPVQKLRRDSYSFLNIDGVQSGKWRALKVNEISRLKRQIE
K

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=34.1880341880342, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=34.9593495934959, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=29.2887029288703, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI87081838, Length=173, Percent_Identity=34.6820809248555, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27293; Mature: 27293

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEERLQKVMAEAGVASRRKSEKLITEGHVRVNGKVIKQLGTKVTAHDKIEVDGVPLQRE
CCHHHHHHHHHHHCHHHHHHHHHHHHCCEEEECHHHHHHHCCEEECCCEEEECCCCCCCC
KMVYFLLNKPRGVITSVSDDKKRKTVMDFFPDVVERIYPVGRLDYDTSGAVLMTNDGTLA
CEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
NGLTHPKFKFEKTYTAKVKGIVNSLDLKKLENGLVIDHKKTAKAKAHLISTDDEKKTSIV
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHEEEECCCCCCCEEEE
SLTIHEGRNHQVKNMFMAVGHPVQKLRRDSYSFLNIDGVQSGKWRALKVNEISRLKRQIE
EEEEECCCCCHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHC
K
C
>Mature Secondary Structure
MEEERLQKVMAEAGVASRRKSEKLITEGHVRVNGKVIKQLGTKVTAHDKIEVDGVPLQRE
CCHHHHHHHHHHHCHHHHHHHHHHHHCCEEEECHHHHHHHCCEEECCCEEEECCCCCCCC
KMVYFLLNKPRGVITSVSDDKKRKTVMDFFPDVVERIYPVGRLDYDTSGAVLMTNDGTLA
CEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
NGLTHPKFKFEKTYTAKVKGIVNSLDLKKLENGLVIDHKKTAKAKAHLISTDDEKKTSIV
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHEEEECCCCCCCEEEE
SLTIHEGRNHQVKNMFMAVGHPVQKLRRDSYSFLNIDGVQSGKWRALKVNEISRLKRQIE
EEEEECCCCCHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]