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The map label for this gene is topA [H]

Identifier: 116492728

GI number: 116492728

Start: 967864

End: 969936

Strand: Reverse

Name: topA [H]

Synonym: PEPE_0965

Alternate gene names: 116492728

Gene position: 969936-967864 (Counterclockwise)

Preceding gene: 116492729

Following gene: 116492727

Centisome position: 52.93

GC content: 39.7

Gene sequence:

>2073_bases
ATGCCAGCAGCGAAAAAAAACCTTGTGATTGTGGAATCACCATCCAAGGCTAAAACCATTGAAAAATATTTAGGCCGTAC
CTATAAAGTAGTTGCGAGTCTTGGACATATTCGTGATCTACCAAAGAGTAAGATGGGCGTGGATCTGGAAAATGATTACG
AACCCCATTACATCTCTATTCGTGGTAAGGGCGATACCATTAAAAATTTACGTAAAGAAGCTAAAAAAGCTAAAAAAGTT
TATCTGGCATCTGATCCCGATCGGGAAGGAGAAGCCATTGCGTGGCACCTTTCATATCTATTAGGGTTGGATCCCAAAGA
TCAAAATCGAGTGGTTTTTAATGAAATTACTAAAGATGCCGTTAAAAATGCGTTCAAAACTCCGCGTAGCATTGATATGA
CGCTCGTTGATGCTCAACAAGCTCGTCGGATTTTGGATCGTTTAGTCGGGTATTCGATTTCTCCATTACTATGGCAAAAA
GTTAAAAAAGGCCTTAGTGCTGGTCGAGTTCAATCAATTGCCTTAAGTTTAATTATTAAACGTGAACAAGAAATCAAGGC
GTTTGAACCTGAAGAATATTGGTCAATTGATTCTGAATTTAAAAAAGGACGCTCTAAATTTAAGGCTGCCTTTTATGGCG
TGAATAATAAAAAGATGAAGCTGCCTAATAATGATAAGGTTCAGGAAGTATTAAAGAAAATCGACCCTAAGGCTGATTTT
GATATTACGGAAGTTAAACGCCGAGAACGCAAACGTTATCCAGCTCCAGCTTTTACAACTAGTTCTATGCAACAAGAAGC
CAACAAGACGTTGAATTTCAGAACTCGTAAAACGATGATGGCTGCCCAACAACTTTATGAAGGAATTAACATTGGTAAAG
AAGGCACTGTCGGTTTGATTACCTATATGCGAACCGACTCGACTCGTATTTCTGAGGTTGCTAAGCACGAAACTTCATTA
TTTATTCATGATGAGTTTGGCGCAGAATATGCTGCTACTAAGCCTCGGAAGGGTAAAATGCCCGAGGGTGCTCAGGATGC
CCATGAAGCAATCCGCCCTTCGTCCGTATTGCGGACTCCTGAAAGTATTAAAGAATACCTTAATAAAGATCAATTTAGAC
TTTATAATTTAATTTGGTCCCGTTTTGTTGCTAGTCAAATGACACCAGAAGTTTCGGATACCATGACGGTTAAGATTGAA
CAAAATGGAGTTCAATTCCGAGCTAACGGATCAAAGACCAAATTTGAAGGTTTCACAAAAGTTTACAAGACCAATAAGGA
CAAAGATAACGTTCTTCCAAATCTAGAAGAGGGCGACCAAGTCCAACTGGTTACCAACAACCCGAACCAACATTTCACTC
AGCCACCAGCTCGCTATAGTGAAGCTACTTTAATTAAAGCTTTGGAAGAAAATGGGGTAGGTCGTCCTTCTACGTACGCT
CCAACCTTAGACACGATTCAAAGGCGTTACTATGTTAAGTTAGAGTCTAAACATTTTGAACCTACTGAACTGGGTGAAAT
CGTCAATGAAATTATTTATGACTTCTTCCCAGATATCGTTAATATCGATTTTACGGCTGGCTTGGAAAAAGACTTGGATT
CGATTGAAGAAGACAAGGAAGACTGGGTGAAAGTCGTTGACCGTTATTACAAACCTTTTGCTAAGGAACTAGCCAAAGCA
CAGGAACAAATCGAAAAAGTTCAGATTAAAGACGAACTAGCTGGTTTTAATTGTGAAATCTGCGGTTCACCCATGGTAGT
CAAAATGGGACGTTACGGTAAGTTCTTCGCTTGCAGCCGTTTCCCAGACTGTCGTAATACCCAAGCAATTGTTAAAGAAA
TTGGCGTAACCTGTCCTAAGTGTCATCAGGGACAAATTATTGAACGTAAATCTAAACGCGGTCGATTGTTCTATGGTTGC
TCTAGGTATCCGGACTGTGACTTTGTGTCATGGGATAAACCAATTGCTCGTGAATGTCCTGTTTGTCATAGCTACTTGAT
TGAAAAGAAAATTAAAGGCGGCAAACAAATTGTTTGTCCAAATGGTGACTACGAAGAAACACCTCAAAAATAG

Upstream 100 bases:

>100_bases
ATTATAACTGGATTAATGATAGTTATTTGACAATATCGAATATTATGCAATAACATGGTGTCTATAAAATTTTAAATATA
ACAAGGCAAAGGAGCAACAA

Downstream 100 bases:

>100_bases
TTTAAAAAACAAAATATTAATTGACGTTTACCTCTAACATTTGCTACAGTGACAAGTGTTGGAGGTTTTTTATGGAATTA
ATTTCACTGTTCAAACAGTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MPAAKKNLVIVESPSKAKTIEKYLGRTYKVVASLGHIRDLPKSKMGVDLENDYEPHYISIRGKGDTIKNLRKEAKKAKKV
YLASDPDREGEAIAWHLSYLLGLDPKDQNRVVFNEITKDAVKNAFKTPRSIDMTLVDAQQARRILDRLVGYSISPLLWQK
VKKGLSAGRVQSIALSLIIKREQEIKAFEPEEYWSIDSEFKKGRSKFKAAFYGVNNKKMKLPNNDKVQEVLKKIDPKADF
DITEVKRRERKRYPAPAFTTSSMQQEANKTLNFRTRKTMMAAQQLYEGINIGKEGTVGLITYMRTDSTRISEVAKHETSL
FIHDEFGAEYAATKPRKGKMPEGAQDAHEAIRPSSVLRTPESIKEYLNKDQFRLYNLIWSRFVASQMTPEVSDTMTVKIE
QNGVQFRANGSKTKFEGFTKVYKTNKDKDNVLPNLEEGDQVQLVTNNPNQHFTQPPARYSEATLIKALEENGVGRPSTYA
PTLDTIQRRYYVKLESKHFEPTELGEIVNEIIYDFFPDIVNIDFTAGLEKDLDSIEEDKEDWVKVVDRYYKPFAKELAKA
QEQIEKVQIKDELAGFNCEICGSPMVVKMGRYGKFFACSRFPDCRNTQAIVKEIGVTCPKCHQGQIIERKSKRGRLFYGC
SRYPDCDFVSWDKPIARECPVCHSYLIEKKIKGGKQIVCPNGDYEETPQK

Sequences:

>Translated_690_residues
MPAAKKNLVIVESPSKAKTIEKYLGRTYKVVASLGHIRDLPKSKMGVDLENDYEPHYISIRGKGDTIKNLRKEAKKAKKV
YLASDPDREGEAIAWHLSYLLGLDPKDQNRVVFNEITKDAVKNAFKTPRSIDMTLVDAQQARRILDRLVGYSISPLLWQK
VKKGLSAGRVQSIALSLIIKREQEIKAFEPEEYWSIDSEFKKGRSKFKAAFYGVNNKKMKLPNNDKVQEVLKKIDPKADF
DITEVKRRERKRYPAPAFTTSSMQQEANKTLNFRTRKTMMAAQQLYEGINIGKEGTVGLITYMRTDSTRISEVAKHETSL
FIHDEFGAEYAATKPRKGKMPEGAQDAHEAIRPSSVLRTPESIKEYLNKDQFRLYNLIWSRFVASQMTPEVSDTMTVKIE
QNGVQFRANGSKTKFEGFTKVYKTNKDKDNVLPNLEEGDQVQLVTNNPNQHFTQPPARYSEATLIKALEENGVGRPSTYA
PTLDTIQRRYYVKLESKHFEPTELGEIVNEIIYDFFPDIVNIDFTAGLEKDLDSIEEDKEDWVKVVDRYYKPFAKELAKA
QEQIEKVQIKDELAGFNCEICGSPMVVKMGRYGKFFACSRFPDCRNTQAIVKEIGVTCPKCHQGQIIERKSKRGRLFYGC
SRYPDCDFVSWDKPIARECPVCHSYLIEKKIKGGKQIVCPNGDYEETPQK
>Mature_689_residues
PAAKKNLVIVESPSKAKTIEKYLGRTYKVVASLGHIRDLPKSKMGVDLENDYEPHYISIRGKGDTIKNLRKEAKKAKKVY
LASDPDREGEAIAWHLSYLLGLDPKDQNRVVFNEITKDAVKNAFKTPRSIDMTLVDAQQARRILDRLVGYSISPLLWQKV
KKGLSAGRVQSIALSLIIKREQEIKAFEPEEYWSIDSEFKKGRSKFKAAFYGVNNKKMKLPNNDKVQEVLKKIDPKADFD
ITEVKRRERKRYPAPAFTTSSMQQEANKTLNFRTRKTMMAAQQLYEGINIGKEGTVGLITYMRTDSTRISEVAKHETSLF
IHDEFGAEYAATKPRKGKMPEGAQDAHEAIRPSSVLRTPESIKEYLNKDQFRLYNLIWSRFVASQMTPEVSDTMTVKIEQ
NGVQFRANGSKTKFEGFTKVYKTNKDKDNVLPNLEEGDQVQLVTNNPNQHFTQPPARYSEATLIKALEENGVGRPSTYAP
TLDTIQRRYYVKLESKHFEPTELGEIVNEIIYDFFPDIVNIDFTAGLEKDLDSIEEDKEDWVKVVDRYYKPFAKELAKAQ
EQIEKVQIKDELAGFNCEICGSPMVVKMGRYGKFFACSRFPDCRNTQAIVKEIGVTCPKCHQGQIIERKSKRGRLFYGCS
RYPDCDFVSWDKPIARECPVCHSYLIEKKIKGGKQIVCPNGDYEETPQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=622, Percent_Identity=26.5273311897106, Blast_Score=154, Evalue=4e-37,
Organism=Homo sapiens, GI4507635, Length=574, Percent_Identity=23.8675958188153, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI1787529, Length=752, Percent_Identity=38.6968085106383, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI48994931, Length=110, Percent_Identity=43.6363636363636, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI1788061, Length=508, Percent_Identity=22.8346456692913, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=550, Percent_Identity=24.1818181818182, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=508, Percent_Identity=23.8188976377953, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=558, Percent_Identity=24.0143369175627, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585251, Length=643, Percent_Identity=24.5723172628305, Blast_Score=131, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640096, Length=510, Percent_Identity=22.9411764705882, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78933; Mature: 78802

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAKKNLVIVESPSKAKTIEKYLGRTYKVVASLGHIRDLPKSKMGVDLENDYEPHYISI
CCCCCCCEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEE
RGKGDTIKNLRKEAKKAKKVYLASDPDREGEAIAWHLSYLLGLDPKDQNRVVFNEITKDA
ECCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEHEEHHHHHCCCCCCCCCCHHHHHHHHH
VKNAFKTPRSIDMTLVDAQQARRILDRLVGYSISPLLWQKVKKGLSAGRVQSIALSLIIK
HHHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
REQEIKAFEPEEYWSIDSEFKKGRSKFKAAFYGVNNKKMKLPNNDKVQEVLKKIDPKADF
HHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCC
DITEVKRRERKRYPAPAFTTSSMQQEANKTLNFRTRKTMMAAQQLYEGINIGKEGTVGLI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
TYMRTDSTRISEVAKHETSLFIHDEFGAEYAATKPRKGKMPEGAQDAHEAIRPSSVLRTP
EEEECCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCH
ESIKEYLNKDQFRLYNLIWSRFVASQMTPEVSDTMTVKIEQNGVQFRANGSKTKFEGFTK
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHH
VYKTNKDKDNVLPNLEEGDQVQLVTNNPNQHFTQPPARYSEATLIKALEENGVGRPSTYA
HHHCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PTLDTIQRRYYVKLESKHFEPTELGEIVNEIIYDFFPDIVNIDFTAGLEKDLDSIEEDKE
CCHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHH
DWVKVVDRYYKPFAKELAKAQEQIEKVQIKDELAGFNCEICGSPMVVKMGRYGKFFACSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCEEEECC
FPDCRNTQAIVKEIGVTCPKCHQGQIIERKSKRGRLFYGCSRYPDCDFVSWDKPIARECP
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHCC
VCHSYLIEKKIKGGKQIVCPNGDYEETPQK
HHHHHHHHHHHCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
PAAKKNLVIVESPSKAKTIEKYLGRTYKVVASLGHIRDLPKSKMGVDLENDYEPHYISI
CCCCCCEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEE
RGKGDTIKNLRKEAKKAKKVYLASDPDREGEAIAWHLSYLLGLDPKDQNRVVFNEITKDA
ECCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEHEEHHHHHCCCCCCCCCCHHHHHHHHH
VKNAFKTPRSIDMTLVDAQQARRILDRLVGYSISPLLWQKVKKGLSAGRVQSIALSLIIK
HHHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
REQEIKAFEPEEYWSIDSEFKKGRSKFKAAFYGVNNKKMKLPNNDKVQEVLKKIDPKADF
HHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCC
DITEVKRRERKRYPAPAFTTSSMQQEANKTLNFRTRKTMMAAQQLYEGINIGKEGTVGLI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
TYMRTDSTRISEVAKHETSLFIHDEFGAEYAATKPRKGKMPEGAQDAHEAIRPSSVLRTP
EEEECCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCH
ESIKEYLNKDQFRLYNLIWSRFVASQMTPEVSDTMTVKIEQNGVQFRANGSKTKFEGFTK
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHH
VYKTNKDKDNVLPNLEEGDQVQLVTNNPNQHFTQPPARYSEATLIKALEENGVGRPSTYA
HHHCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PTLDTIQRRYYVKLESKHFEPTELGEIVNEIIYDFFPDIVNIDFTAGLEKDLDSIEEDKE
CCHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHH
DWVKVVDRYYKPFAKELAKAQEQIEKVQIKDELAGFNCEICGSPMVVKMGRYGKFFACSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCEEEECC
FPDCRNTQAIVKEIGVTCPKCHQGQIIERKSKRGRLFYGCSRYPDCDFVSWDKPIARECP
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHCC
VCHSYLIEKKIKGGKQIVCPNGDYEETPQK
HHHHHHHHHHHCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]