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The map label for this gene is eno

Identifier: 116492264

GI number: 116492264

Start: 515455

End: 516777

Strand: Direct

Name: eno

Synonym: PEPE_0462

Alternate gene names: 116492264

Gene position: 515455-516777 (Clockwise)

Preceding gene: 116492263

Following gene: 116492265

Centisome position: 28.13

GC content: 41.95

Gene sequence:

>1323_bases
ATGTCACTTATTACTGATATTTACGCACGCGAAGTCCTAGACTCACGTGGTAACCCAACTGTTGAAGTTGAGGTTTATAC
TGAAGATGGTGGTTTCGGTCGCGGAATCGTTCCTTCTGGTGCTTCAACTGGTGAACACGAAGCTGTTGAACTTCGTGATG
GCGACAAGAACCGTTTCTCAGGAAAAGGTGTAGAAAAGGCTGTTGCTAACGTTAATGGTCCTATTTCTAAAGAAATCGTT
GGTTTTGAAGTTACAGACCAAATCGCTATCGATAAGGCAATGATTAAATTAGACGGTACCCCTAACAAGGGTAAGCTTGG
TGCTAACGCTATCTTAGGTGTATCACTTGCTGTTGCTCGTGCAGCTGCAGATGAACTACAAGTTCCTCTATACAACTACA
TTGGCGGTTCAAACGCTCATGTTCTTCCAACACCAATGATGAATGTTATCAATGGTGGAGCTCACTCAGAAAACAAAGTG
GACTTCCAAGAATTCATGATCATGCCTGTTGGTGCTCCTTCTGTTAAAGAAGCTATCCGTATGGGTTCAGAAACTTTCCA
TGCTTTGAAGAGTCTTTTGTCAGCTGATGGCAAAGCTACTTCAGTTGGTGATGAAGGTGGATTTGCTCCTGACTTTGCTA
ACAATGAAGAACCTCTTCAATACCTAATCAAGGCTATTGAAAAGGCTGGTTACAAACCAGGTAAGGACGTTGCTATTGCA
GTTGACGTTGCTGCTTCAGAACTTTGGAACGACGAAGACAAGAAGTACAAGCTTCGCTGGTCAACTGGTGAAGAATTCAC
TACTGAAGAATTCGTTAAGTACCTTGAAGGCTTAGTAAACAAATACCCAATCATTTCTATCGAAGATCCTATCGATGAAA
ACAACTGGGACGACTGGGCAAGCATCACTAAAGAACTTGGCAAGAAAGTTCAACTTGTTGGTGACGACTTCTTCGTTACA
AACACAGAATACCTTGCTAAAGGTATCAAGATGGGTGCTGCTAACGCAATCCTTATCAAGGTTAACCAAATCGGTACTCT
TACAGAAACAATGGAATCTATCGAAATGGCTAAAGAAGCTGGTTACACAGCTATCGTTTCTCACCGTTCAGGTGAAACAG
AAGACACAACAATCGCTGACTTAGTTGTTGCTACAAATGCAGGTCAAATTAAGACTGGTTCAATGAGTCGTACAGACCGT
ATTGCTAAGTACAACCAATTAATGCGTATTGAAGACCAACTTGAAGGCGTTGCAGAATACAAGGGTATTAACTCATTCTA
CAACCTAAGTGCTGACGCTCGTGAAGAAATCAGCAACAAGTAA

Upstream 100 bases:

>100_bases
AGAATTAGTTAACTACAAGGGTTAATATAATTAAGGAAATATAATATAATAATAGTTGAACTGCGTTAAGCAGATTTAAA
AATGTGAGGAGATATTTAAA

Downstream 100 bases:

>100_bases
TTAAATTAGGTCAATCAAAGAGGCGGACATTTTGTCCGCCTCTTTTTTTGTTATTAAATTGGAATTCAGGCTCAAATATG
TTAAATTAGTACTGGAACTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSLITDIYAREVLDSRGNPTVEVEVYTEDGGFGRGIVPSGASTGEHEAVELRDGDKNRFSGKGVEKAVANVNGPISKEIV
GFEVTDQIAIDKAMIKLDGTPNKGKLGANAILGVSLAVARAAADELQVPLYNYIGGSNAHVLPTPMMNVINGGAHSENKV
DFQEFMIMPVGAPSVKEAIRMGSETFHALKSLLSADGKATSVGDEGGFAPDFANNEEPLQYLIKAIEKAGYKPGKDVAIA
VDVAASELWNDEDKKYKLRWSTGEEFTTEEFVKYLEGLVNKYPIISIEDPIDENNWDDWASITKELGKKVQLVGDDFFVT
NTEYLAKGIKMGAANAILIKVNQIGTLTETMESIEMAKEAGYTAIVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDR
IAKYNQLMRIEDQLEGVAEYKGINSFYNLSADAREEISNK

Sequences:

>Translated_440_residues
MSLITDIYAREVLDSRGNPTVEVEVYTEDGGFGRGIVPSGASTGEHEAVELRDGDKNRFSGKGVEKAVANVNGPISKEIV
GFEVTDQIAIDKAMIKLDGTPNKGKLGANAILGVSLAVARAAADELQVPLYNYIGGSNAHVLPTPMMNVINGGAHSENKV
DFQEFMIMPVGAPSVKEAIRMGSETFHALKSLLSADGKATSVGDEGGFAPDFANNEEPLQYLIKAIEKAGYKPGKDVAIA
VDVAASELWNDEDKKYKLRWSTGEEFTTEEFVKYLEGLVNKYPIISIEDPIDENNWDDWASITKELGKKVQLVGDDFFVT
NTEYLAKGIKMGAANAILIKVNQIGTLTETMESIEMAKEAGYTAIVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDR
IAKYNQLMRIEDQLEGVAEYKGINSFYNLSADAREEISNK
>Mature_439_residues
SLITDIYAREVLDSRGNPTVEVEVYTEDGGFGRGIVPSGASTGEHEAVELRDGDKNRFSGKGVEKAVANVNGPISKEIVG
FEVTDQIAIDKAMIKLDGTPNKGKLGANAILGVSLAVARAAADELQVPLYNYIGGSNAHVLPTPMMNVINGGAHSENKVD
FQEFMIMPVGAPSVKEAIRMGSETFHALKSLLSADGKATSVGDEGGFAPDFANNEEPLQYLIKAIEKAGYKPGKDVAIAV
DVAASELWNDEDKKYKLRWSTGEEFTTEEFVKYLEGLVNKYPIISIEDPIDENNWDDWASITKELGKKVQLVGDDFFVTN
TEYLAKGIKMGAANAILIKVNQIGTLTETMESIEMAKEAGYTAIVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDRI
AKYNQLMRIEDQLEGVAEYKGINSFYNLSADAREEISNK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=438, Percent_Identity=50.2283105022831, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=439, Percent_Identity=51.25284738041, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897469, Length=439, Percent_Identity=51.25284738041, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=47.196261682243, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI169201331, Length=352, Percent_Identity=25, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI169201757, Length=352, Percent_Identity=25, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI239744207, Length=352, Percent_Identity=25, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=60.3286384976526, Blast_Score=499, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71995829, Length=438, Percent_Identity=51.5981735159817, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=51.5981735159817, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=198, Percent_Identity=43.4343434343434, Blast_Score=168, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.4226327944573, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.4226327944573, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.4226327944573, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321693, Length=440, Percent_Identity=49.3181818181818, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=435, Percent_Identity=49.6551724137931, Blast_Score=359, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=49.3119266055046, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=49.3119266055046, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=49.3119266055046, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=49.3119266055046, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=436, Percent_Identity=49.3119266055046, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=436, Percent_Identity=49.3119266055046, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PEDPA (Q03GW5)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803999.1
- ProteinModelPortal:   Q03GW5
- SMR:   Q03GW5
- STRING:   Q03GW5
- GeneID:   4418359
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0462
- NMPDR:   fig|278197.10.peg.430
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q03GW5
- ProtClustDB:   PRK00077
- BioCyc:   PPEN278197:PEPE_0462-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47667; Mature: 47536

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 340-340 BINDING 155-155 BINDING 164-164 BINDING 288-288 BINDING 315-315 BINDING 340-340 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLITDIYAREVLDSRGNPTVEVEVYTEDGGFGRGIVPSGASTGEHEAVELRDGDKNRFS
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVEKAVANVNGPISKEIVGFEVTDQIAIDKAMIKLDGTPNKGKLGANAILGVSLAVAR
CCCHHHHHHHCCCCCCCCEECEEEHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADELQVPLYNYIGGSNAHVLPTPMMNVINGGAHSENKVDFQEFMIMPVGAPSVKEAIR
HHHHHCCCCHHHHCCCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGSETFHALKSLLSADGKATSVGDEGGFAPDFANNEEPLQYLIKAIEKAGYKPGKDVAIA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
VDVAASELWNDEDKKYKLRWSTGEEFTTEEFVKYLEGLVNKYPIISIEDPIDENNWDDWA
EEEHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
SITKELGKKVQLVGDDFFVTNTEYLAKGIKMGAANAILIKVNQIGTLTETMESIEMAKEA
HHHHHHCCEEEEECCCEEEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAIVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDRIAKYNQLMRIEDQLEGVAEY
CCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGINSFYNLSADAREEISNK
CCCCHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
SLITDIYAREVLDSRGNPTVEVEVYTEDGGFGRGIVPSGASTGEHEAVELRDGDKNRFS
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVEKAVANVNGPISKEIVGFEVTDQIAIDKAMIKLDGTPNKGKLGANAILGVSLAVAR
CCCHHHHHHHCCCCCCCCEECEEEHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADELQVPLYNYIGGSNAHVLPTPMMNVINGGAHSENKVDFQEFMIMPVGAPSVKEAIR
HHHHHCCCCHHHHCCCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGSETFHALKSLLSADGKATSVGDEGGFAPDFANNEEPLQYLIKAIEKAGYKPGKDVAIA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
VDVAASELWNDEDKKYKLRWSTGEEFTTEEFVKYLEGLVNKYPIISIEDPIDENNWDDWA
EEEHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
SITKELGKKVQLVGDDFFVTNTEYLAKGIKMGAANAILIKVNQIGTLTETMESIEMAKEA
HHHHHHCCEEEEECCCEEEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAIVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDRIAKYNQLMRIEDQLEGVAEY
CCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGINSFYNLSADAREEISNK
CCCCHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA