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The map label for this gene is pyrB

Identifier: 116492116

GI number: 116492116

Start: 357057

End: 357983

Strand: Direct

Name: pyrB

Synonym: PEPE_0313

Alternate gene names: 116492116

Gene position: 357057-357983 (Clockwise)

Preceding gene: 116492115

Following gene: 116492117

Centisome position: 19.49

GC content: 39.81

Gene sequence:

>927_bases
ATGAATATGCATGACTTAACCACTGTAGAAAGCTTTAGTGAAGAGGACGTATTACATAAAATTCGGTTAGCTGAGGAATT
TAAGAATGGTAAGACAATTGAACTTACACGACCGGTCTATGCAATGAACTTATTTTTTGAAAATAGTACCCGAACTCATA
CTAGTTTTGAAATGGCAGAAAGTCGTCTAGGAATGCGACTCCTTGAGTTTGAAGCTAAAACTAGTTCAGTTACTAAAGGG
GAAAGTTTATTGGATACGGTTAAGACAATTGATGCAATTCAGACGGATATTGCAGTCATCCGTCATCCGCAAAACGATTA
TTATAAACAACTGCTAGAAGCCGATTTGAATATTAGTATTGTCAACGGAGGCGATGGAAGTGGACAACATCCCTCACAAT
CATTACTCGACATGATGACCATTTATCAAGAATTTAACCACTTTGACGGTTTAAAAATTGCGATTGCCGGAGATATCGCA
CATTCCCGAGTGGCCCGCTCCAATGCAATGCTATTAAACAAGCTGGGTGCAGAAGTTTACTTCGCCGGTCCTGAAGCATG
GATGGCCGAAGATTTGAAGGCTTATGGAACCTTCCTACCAATTGACGAATTAGTAGAGAAAGTGGACGTCATGATGTTAC
TCCGAATTCAAAATGAACGTATTTCTCAAGATACCGCAATTAAATTTGAACCACACGAATATCTCCAAGAATATGGATTA
ACCTTCGAACGAGCCGATCGAATGCAACCAACTGCTATCATTATGCATCCCGCCCCAGTTAATCGTGGTACAGAAATCGA
AAGCAGCTTGGTTGATGGAGAAAAATCAAGAATTTTCAAGCAAATGACTAATGGTATGTACATGCGCATGGCAATTTTAA
CGGATGTATTAGAGGCCAAGGGATTGATTAAGGGAGGATTGCATTAA

Upstream 100 bases:

>100_bases
TCCCGAGAGGAACCAATTGAATCGGCTTTTTAAGCTACTCCCAAGCGAGACGATTCGATTGGTGAGTAGCTTTTTTTATA
ATAATTTAGGAGGAAAAATA

Downstream 100 bases:

>100_bases
TGAAAGTTTTACTTAAAAATGCCCGAGTAATAAAAAGTCATCAAGTGCAAAAAACTGACGTCCTTGTGGAAAACGACCGA
ATCACTCAAATTCAACCCGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNMHDLTTVESFSEEDVLHKIRLAEEFKNGKTIELTRPVYAMNLFFENSTRTHTSFEMAESRLGMRLLEFEAKTSSVTKG
ESLLDTVKTIDAIQTDIAVIRHPQNDYYKQLLEADLNISIVNGGDGSGQHPSQSLLDMMTIYQEFNHFDGLKIAIAGDIA
HSRVARSNAMLLNKLGAEVYFAGPEAWMAEDLKAYGTFLPIDELVEKVDVMMLLRIQNERISQDTAIKFEPHEYLQEYGL
TFERADRMQPTAIIMHPAPVNRGTEIESSLVDGEKSRIFKQMTNGMYMRMAILTDVLEAKGLIKGGLH

Sequences:

>Translated_308_residues
MNMHDLTTVESFSEEDVLHKIRLAEEFKNGKTIELTRPVYAMNLFFENSTRTHTSFEMAESRLGMRLLEFEAKTSSVTKG
ESLLDTVKTIDAIQTDIAVIRHPQNDYYKQLLEADLNISIVNGGDGSGQHPSQSLLDMMTIYQEFNHFDGLKIAIAGDIA
HSRVARSNAMLLNKLGAEVYFAGPEAWMAEDLKAYGTFLPIDELVEKVDVMMLLRIQNERISQDTAIKFEPHEYLQEYGL
TFERADRMQPTAIIMHPAPVNRGTEIESSLVDGEKSRIFKQMTNGMYMRMAILTDVLEAKGLIKGGLH
>Mature_308_residues
MNMHDLTTVESFSEEDVLHKIRLAEEFKNGKTIELTRPVYAMNLFFENSTRTHTSFEMAESRLGMRLLEFEAKTSSVTKG
ESLLDTVKTIDAIQTDIAVIRHPQNDYYKQLLEADLNISIVNGGDGSGQHPSQSLLDMMTIYQEFNHFDGLKIAIAGDIA
HSRVARSNAMLLNKLGAEVYFAGPEAWMAEDLKAYGTFLPIDELVEKVDVMMLLRIQNERISQDTAIKFEPHEYLQEYGL
TFERADRMQPTAIIMHPAPVNRGTEIESSLVDGEKSRIFKQMTNGMYMRMAILTDVLEAKGLIKGGLH

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=33.4415584415584, Blast_Score=159, Evalue=2e-39,
Organism=Escherichia coli, GI2367364, Length=307, Percent_Identity=31.2703583061889, Blast_Score=137, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI193204318, Length=278, Percent_Identity=34.5323741007194, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=268, Percent_Identity=31.3432835820896, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24642586, Length=267, Percent_Identity=32.5842696629214, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PEDPA (Q03HB3)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803851.1
- ProteinModelPortal:   Q03HB3
- SMR:   Q03HB3
- STRING:   Q03HB3
- GeneID:   4418489
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0313
- NMPDR:   fig|278197.10.peg.288
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   PPEN278197:PEPE_0313-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34828; Mature: 34828

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMHDLTTVESFSEEDVLHKIRLAEEFKNGKTIELTRPVYAMNLFFENSTRTHTSFEMAE
CCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEECCCCCHHHHHHHH
SRLGMRLLEFEAKTSSVTKGESLLDTVKTIDAIQTDIAVIRHPQNDYYKQLLEADLNISI
HHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCEEE
VNGGDGSGQHPSQSLLDMMTIYQEFNHFDGLKIAIAGDIAHSRVARSNAMLLNKLGAEVY
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEE
FAGPEAWMAEDLKAYGTFLPIDELVEKVDVMMLLRIQNERISQDTAIKFEPHEYLQEYGL
EECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCC
TFERADRMQPTAIIMHPAPVNRGTEIESSLVDGEKSRIFKQMTNGMYMRMAILTDVLEAK
CHHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLIKGGLH
HHHCCCCC
>Mature Secondary Structure
MNMHDLTTVESFSEEDVLHKIRLAEEFKNGKTIELTRPVYAMNLFFENSTRTHTSFEMAE
CCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEECCCCCHHHHHHHH
SRLGMRLLEFEAKTSSVTKGESLLDTVKTIDAIQTDIAVIRHPQNDYYKQLLEADLNISI
HHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCEEE
VNGGDGSGQHPSQSLLDMMTIYQEFNHFDGLKIAIAGDIAHSRVARSNAMLLNKLGAEVY
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEE
FAGPEAWMAEDLKAYGTFLPIDELVEKVDVMMLLRIQNERISQDTAIKFEPHEYLQEYGL
EECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCC
TFERADRMQPTAIIMHPAPVNRGTEIESSLVDGEKSRIFKQMTNGMYMRMAILTDVLEAK
CHHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLIKGGLH
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA