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The map label for this gene is lmrB [H]

Identifier: 116492079

GI number: 116492079

Start: 320054

End: 321493

Strand: Direct

Name: lmrB [H]

Synonym: PEPE_0275

Alternate gene names: 116492079

Gene position: 320054-321493 (Clockwise)

Preceding gene: 116492078

Following gene: 116492085

Centisome position: 17.47

GC content: 36.39

Gene sequence:

>1440_bases
ATGAATAATGAAGAAAAAAGCAACGGCATGACGCAAGCGCGAGTACCAGTTGAACATCCAATGTTGGTAATGGCGAGCAT
GTTGATTGGGGCCTTTGTGGGAATGTTTAGTGAGACATCTTTAAACATCGCTTTACCACAATTAATGGACAATTTACATG
TGACGACGGGTACTATCCAATGGTTAGTTACGGGATACATGCTTGTGATTGGTGTAGTATTGCCCTTTTCTAGTTTAATA
TCAAAATGGTTTACAACTAGACAGATTATTATTTTTGGTTTATTAGCGTTTATTGTAGGTTCTGTTATTTCAGCTCTATC
ATCAACCTTTGCATTATTACTGGTTGGTCGAATGATTCAAGGAATCGCTACTGGTTTAATTTTACCTATGATGTTTACCG
TAGCCATGTTAGTATTTCCACCTTATAAATTAGGTGCTGCAATGGGCATGTGTGCTTTGGTAATTATGTTTGCTCCAGCC
ATTGGACCGACATTGACAGGTATTATTTTAGCTAAGTTATCATGGAACTGGATTTTTTGGATGTTCGTTCCTTTCTTAGC
AATTGCTTTGTTACTAGCTATTTTTGGATTAAAAAATGTTGGTGAACTTACTAAACCTAAAGTTGATGTAATTTCATTGA
TTGAATCAATTATCGGCTTCGCTAGTATAGTAACTGGTGTCAGTTTTGCTAGTGAAAAAGGCTGGGGTTCGCCAATCGTA
CTAGGTATCTTAGCACTTGGAATTATCGTTTTGGCATTTTATGTCAAACGTCAACTTTCGCTTGCTGAACCTGTCCTAAA
TTTAAAGATTTTTGCCATTCCTGCATTTAGAACTGGTGCAATCTTAGTTATGCTTGATTTTGGTATTATTTTATCAGCAA
TGTACTTATTACCAATGTACATTCAAAAAGGTTTATTATTACCAGTTGCACTAACTGGAATTATCATGCTTCCTGGTGGT
GTAATTAATGCCGCTGTTTCAGCGGTTGCAGGACGTTTATACGACAAGATCGGTGCCAAAGCACCAATTCGTATTGGACT
AGTGATTGCATTAATTGGTGCAATTATGTTGGTATGTACTACGGTAAATACTTCAATTGCTTATTTAATTATTGCTCATG
TTATTTTGATGATTGGTTGTCCTTTGGCAATGTCACCAGCTCAAACATATGCTTTAAATTCGCTTGAAGGTCCCCAATCT
GGTGACGGTAGTACGATCATGAATACCATGCAACAAATCGTTGGTGCGATTGCAACTGCTTTAGCAACAAGTTTATTAGG
AATCGGACAAGTAGCTTATCAGAATCATTTTGGTAAAGGTCATTTGAATGCTGCCTTCACTAATGGTACACACTATGGTT
TCTACTTTACGATTGCATTAATTATCGTAGGATTAATTCTTTCCTTTAGTGTTAAAAGTAATAAAAAAGTTGCCAAATAA

Upstream 100 bases:

>100_bases
GAACGGCCTCTTTTTATTTTTCTCAAAAAAATACTTACGAAAATTTTTTCTTTTTGCTATAGTGAACAAGCTATGTTTTT
GAAGAAAGAGGTATTTTTTA

Downstream 100 bases:

>100_bases
ATAAAAAAGATGAACGAGAATTCTGAATCAGAAATCTCAGTTCATCTTTTTTTGTTGAGTAATTATTTTGCGTCGTAAGC
TTGTTGGAAAAGTTCCACAA

Product: major facilitator superfamily permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNNEEKSNGMTQARVPVEHPMLVMASMLIGAFVGMFSETSLNIALPQLMDNLHVTTGTIQWLVTGYMLVIGVVLPFSSLI
SKWFTTRQIIIFGLLAFIVGSVISALSSTFALLLVGRMIQGIATGLILPMMFTVAMLVFPPYKLGAAMGMCALVIMFAPA
IGPTLTGIILAKLSWNWIFWMFVPFLAIALLLAIFGLKNVGELTKPKVDVISLIESIIGFASIVTGVSFASEKGWGSPIV
LGILALGIIVLAFYVKRQLSLAEPVLNLKIFAIPAFRTGAILVMLDFGIILSAMYLLPMYIQKGLLLPVALTGIIMLPGG
VINAAVSAVAGRLYDKIGAKAPIRIGLVIALIGAIMLVCTTVNTSIAYLIIAHVILMIGCPLAMSPAQTYALNSLEGPQS
GDGSTIMNTMQQIVGAIATALATSLLGIGQVAYQNHFGKGHLNAAFTNGTHYGFYFTIALIIVGLILSFSVKSNKKVAK

Sequences:

>Translated_479_residues
MNNEEKSNGMTQARVPVEHPMLVMASMLIGAFVGMFSETSLNIALPQLMDNLHVTTGTIQWLVTGYMLVIGVVLPFSSLI
SKWFTTRQIIIFGLLAFIVGSVISALSSTFALLLVGRMIQGIATGLILPMMFTVAMLVFPPYKLGAAMGMCALVIMFAPA
IGPTLTGIILAKLSWNWIFWMFVPFLAIALLLAIFGLKNVGELTKPKVDVISLIESIIGFASIVTGVSFASEKGWGSPIV
LGILALGIIVLAFYVKRQLSLAEPVLNLKIFAIPAFRTGAILVMLDFGIILSAMYLLPMYIQKGLLLPVALTGIIMLPGG
VINAAVSAVAGRLYDKIGAKAPIRIGLVIALIGAIMLVCTTVNTSIAYLIIAHVILMIGCPLAMSPAQTYALNSLEGPQS
GDGSTIMNTMQQIVGAIATALATSLLGIGQVAYQNHFGKGHLNAAFTNGTHYGFYFTIALIIVGLILSFSVKSNKKVAK
>Mature_479_residues
MNNEEKSNGMTQARVPVEHPMLVMASMLIGAFVGMFSETSLNIALPQLMDNLHVTTGTIQWLVTGYMLVIGVVLPFSSLI
SKWFTTRQIIIFGLLAFIVGSVISALSSTFALLLVGRMIQGIATGLILPMMFTVAMLVFPPYKLGAAMGMCALVIMFAPA
IGPTLTGIILAKLSWNWIFWMFVPFLAIALLLAIFGLKNVGELTKPKVDVISLIESIIGFASIVTGVSFASEKGWGSPIV
LGILALGIIVLAFYVKRQLSLAEPVLNLKIFAIPAFRTGAILVMLDFGIILSAMYLLPMYIQKGLLLPVALTGIIMLPGG
VINAAVSAVAGRLYDKIGAKAPIRIGLVIALIGAIMLVCTTVNTSIAYLIIAHVILMIGCPLAMSPAQTYALNSLEGPQS
GDGSTIMNTMQQIVGAIATALATSLLGIGQVAYQNHFGKGHLNAAFTNGTHYGFYFTIALIIVGLILSFSVKSNKKVAK

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=28.6407766990291, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789042, Length=420, Percent_Identity=22.1428571428571, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=24.5700245700246, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788710, Length=434, Percent_Identity=20.2764976958525, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788392, Length=443, Percent_Identity=24.3792325056433, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=290, Percent_Identity=26.2068965517241, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50928; Mature: 50928

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]