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The map label for this gene is metG [H]

Identifier: 116492070

GI number: 116492070

Start: 312622

End: 314643

Strand: Direct

Name: metG [H]

Synonym: PEPE_0266

Alternate gene names: 116492070

Gene position: 312622-314643 (Clockwise)

Preceding gene: 116492069

Following gene: 116492071

Centisome position: 17.06

GC content: 37.88

Gene sequence:

>2022_bases
ATGGCAGAAAATAACACATACTATATTACTACGCCGATTTATTACCCATCGGGAAAACTTCATATCGGTAATTCTTATAC
AACAATTGCTTGTGATGCTGAAGCTCGCTTTCAACGTTTAGAAGGAAAGGACGTTTTCTTCTTAACTGGAACAGACGAAC
ACGGTTTAAAAATCGAACAAAAAGCTGAAGCTTTAAACACTACTCCTAAAGCATATGTTGACGGCATGGCAGAGGATATC
AAAAAACTTTGGGATTTACTTGAAATTACTAATGATAAGTTCATCCGTACCACAGATGATTATCATGAAAAAGCTGTTCA
AAAGATTTTTCAAAAATTATTAGATCAAGGCGATATCTACCTTGGTGAATACGAAGGTTGGTATTCAGTTTCTGATGAAG
AATACTTTACTGAATCTCAATTAGCTGAAGTTTACCGTGATGCTGACGGCAACGTAACTGGTGGAAAGGCTCCTTCTGGA
AACGAAGTCGAATTAGTTAAGGAAGAATCTTACTTCTTTAAGATGAGCAAATATGCCGATTGGTTATTAGATTACTACCA
AACTCATCCTAATTTTATTCAACCGGCAAGCCGAATGAATGAAATGATTAATAACTTTATCAAACCTGGTCTAGAAGATC
TTGCGGTTACACGAACTGGTTTTAAGTGGGGAGTTCCAATTAAAAATGACCCTAAACATGTCATTTACGTGTGGATTGAT
GCGTTGTCTAACTATATTACCGCATTGGGTTATAGCAGTGACGAAGATGGTAACTTTAAGAAATTCTGGCCTGCCAATGT
TCATATGGTTGGAAAAGAAATTGTCCGTTTTCATACAATTTACTGGCCAATTATGTTGCACGCCTTAGGCCTCGAACAAC
CCGACCAAATTTATGGTCACGGTTGGTTATTGATGAAGGACAGTAAAATGTCTAAGTCAAAGGGTAATGTTATTTACCCT
GAAACGATTGTTAAAAACTATGGTTTGGATGCTTTACGCTACTACTTATTGAAAGCTATGCCTTACGGAAATGATGGTAA
CTTTACTCCGGAAGATTTTATTTCAAAAATTAACTTTGATTTAGCCAATGATTTAGGTAATCTTTTGAATCGTACGGTAG
CGATGATTAACAAGTATGAAGGCGGCGTTATTCCAACTTTCCAAAGTGGCGTTAATGACGTTGATGTAGATTTGGAAACC
ACAGCTGCTACGACAATTAAGAACTATGAAGACTTAATGAACAACCTTCATTTTGCAGATGCTTTAGCTGAAATTTGGAA
ATTAGTTGCGCGTACAAACAAGTATATTGATGAAACTACGCCTTGGATTCTTGCACGAAATGATGACGACGAATCTAAAC
AAAAATTAGCAAGTGTCATGAATCATTTGGCTGAAAGTTTGCGTGTAATTGCAATCTTGATCCAACCGATGATGACTCAG
TCACCAGCTAAAATCTTTGCACAATTGGGCTTGGATGTTAACCAACTTGAACTTCAAGGTTTGAAGTTTGGTGAAATTCC
TGCTGATACAAAGGTCGTGGAAAAGGGAACTCCAATTTTCCCTCGTTTGGATGTTGAAAAAGAAGTTGAGTTCCTTAAAA
ACCAAATGACTCAATCAGACAAGAAAAAAGGGCGAGCTGCAATGGCTGAAGCTAACCAGAAAAAGGCTGAAGCTGTGGCA
ACAGACCAGAAAAAAGAAATTCGGATCGAAGCTTTTGATAAGGTAGAACTTCGAGTAGCTGAAGTTCTTGAAGCAGGTCC
AGTAAAGGGTGCTGACAAGTTATTGAAGTTTAAGTTAGATGCTGGTGATGAAGAACCACGCCAAATTCTTTCGGGAATTG
CTCAATCATATCCTGATCCAAGCGTTTTAGTAGGTAAAAAAGTTGTGATTGTTGCTAACTTGAAACCACGTAAGATGCGT
GGGGAAATGAGTCAAGGAATGATTTTATCAACTGAACATGACGACCAAGTAAAAGTCGTGATGGTCGACGACCAACACCA
AAACGGCGATGTTTTAGGTTAA

Upstream 100 bases:

>100_bases
GATTAATAAATCGTTATTTTCGTTAAAGAGACGGGTTTTTGACGCTATAAAATATGATACAATTACAATTAATTAACTTG
ACTAGAATAGAGGAACCATA

Downstream 100 bases:

>100_bases
AAAGATTTAACACAATGGGACTGGTGATGAAACAGGTGTTTTGTCCCAGCCCTTTTTATATTCAAGGAGGCAAAAATGCA
TATTTTTGATTCACACACGC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MAENNTYYITTPIYYPSGKLHIGNSYTTIACDAEARFQRLEGKDVFFLTGTDEHGLKIEQKAEALNTTPKAYVDGMAEDI
KKLWDLLEITNDKFIRTTDDYHEKAVQKIFQKLLDQGDIYLGEYEGWYSVSDEEYFTESQLAEVYRDADGNVTGGKAPSG
NEVELVKEESYFFKMSKYADWLLDYYQTHPNFIQPASRMNEMINNFIKPGLEDLAVTRTGFKWGVPIKNDPKHVIYVWID
ALSNYITALGYSSDEDGNFKKFWPANVHMVGKEIVRFHTIYWPIMLHALGLEQPDQIYGHGWLLMKDSKMSKSKGNVIYP
ETIVKNYGLDALRYYLLKAMPYGNDGNFTPEDFISKINFDLANDLGNLLNRTVAMINKYEGGVIPTFQSGVNDVDVDLET
TAATTIKNYEDLMNNLHFADALAEIWKLVARTNKYIDETTPWILARNDDDESKQKLASVMNHLAESLRVIAILIQPMMTQ
SPAKIFAQLGLDVNQLELQGLKFGEIPADTKVVEKGTPIFPRLDVEKEVEFLKNQMTQSDKKKGRAAMAEANQKKAEAVA
TDQKKEIRIEAFDKVELRVAEVLEAGPVKGADKLLKFKLDAGDEEPRQILSGIAQSYPDPSVLVGKKVVIVANLKPRKMR
GEMSQGMILSTEHDDQVKVVMVDDQHQNGDVLG

Sequences:

>Translated_673_residues
MAENNTYYITTPIYYPSGKLHIGNSYTTIACDAEARFQRLEGKDVFFLTGTDEHGLKIEQKAEALNTTPKAYVDGMAEDI
KKLWDLLEITNDKFIRTTDDYHEKAVQKIFQKLLDQGDIYLGEYEGWYSVSDEEYFTESQLAEVYRDADGNVTGGKAPSG
NEVELVKEESYFFKMSKYADWLLDYYQTHPNFIQPASRMNEMINNFIKPGLEDLAVTRTGFKWGVPIKNDPKHVIYVWID
ALSNYITALGYSSDEDGNFKKFWPANVHMVGKEIVRFHTIYWPIMLHALGLEQPDQIYGHGWLLMKDSKMSKSKGNVIYP
ETIVKNYGLDALRYYLLKAMPYGNDGNFTPEDFISKINFDLANDLGNLLNRTVAMINKYEGGVIPTFQSGVNDVDVDLET
TAATTIKNYEDLMNNLHFADALAEIWKLVARTNKYIDETTPWILARNDDDESKQKLASVMNHLAESLRVIAILIQPMMTQ
SPAKIFAQLGLDVNQLELQGLKFGEIPADTKVVEKGTPIFPRLDVEKEVEFLKNQMTQSDKKKGRAAMAEANQKKAEAVA
TDQKKEIRIEAFDKVELRVAEVLEAGPVKGADKLLKFKLDAGDEEPRQILSGIAQSYPDPSVLVGKKVVIVANLKPRKMR
GEMSQGMILSTEHDDQVKVVMVDDQHQNGDVLG
>Mature_672_residues
AENNTYYITTPIYYPSGKLHIGNSYTTIACDAEARFQRLEGKDVFFLTGTDEHGLKIEQKAEALNTTPKAYVDGMAEDIK
KLWDLLEITNDKFIRTTDDYHEKAVQKIFQKLLDQGDIYLGEYEGWYSVSDEEYFTESQLAEVYRDADGNVTGGKAPSGN
EVELVKEESYFFKMSKYADWLLDYYQTHPNFIQPASRMNEMINNFIKPGLEDLAVTRTGFKWGVPIKNDPKHVIYVWIDA
LSNYITALGYSSDEDGNFKKFWPANVHMVGKEIVRFHTIYWPIMLHALGLEQPDQIYGHGWLLMKDSKMSKSKGNVIYPE
TIVKNYGLDALRYYLLKAMPYGNDGNFTPEDFISKINFDLANDLGNLLNRTVAMINKYEGGVIPTFQSGVNDVDVDLETT
AATTIKNYEDLMNNLHFADALAEIWKLVARTNKYIDETTPWILARNDDDESKQKLASVMNHLAESLRVIAILIQPMMTQS
PAKIFAQLGLDVNQLELQGLKFGEIPADTKVVEKGTPIFPRLDVEKEVEFLKNQMTQSDKKKGRAAMAEANQKKAEAVAT
DQKKEIRIEAFDKVELRVAEVLEAGPVKGADKLLKFKLDAGDEEPRQILSGIAQSYPDPSVLVGKKVVIVANLKPRKMRG
EMSQGMILSTEHDDQVKVVMVDDQHQNGDVLG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=548, Percent_Identity=33.5766423357664, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI14043022, Length=609, Percent_Identity=26.1083743842365, Blast_Score=157, Evalue=4e-38,
Organism=Escherichia coli, GI1788432, Length=677, Percent_Identity=27.3264401772526, Blast_Score=254, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI212645096, Length=522, Percent_Identity=31.6091954022989, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71992781, Length=416, Percent_Identity=29.0865384615385, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17540794, Length=602, Percent_Identity=26.4119601328904, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321610, Length=518, Percent_Identity=32.8185328185328, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321704, Length=517, Percent_Identity=25.9187620889749, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21358483, Length=503, Percent_Identity=32.8031809145129, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24655311, Length=608, Percent_Identity=24.8355263157895, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76186; Mature: 76055

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENNTYYITTPIYYPSGKLHIGNSYTTIACDAEARFQRLEGKDVFFLTGTDEHGLKIEQ
CCCCCEEEEECCEEECCCEEEECCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCEEHH
KAEALNTTPKAYVDGMAEDIKKLWDLLEITNDKFIRTTDDYHEKAVQKIFQKLLDQGDIY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCEE
LGEYEGWYSVSDEEYFTESQLAEVYRDADGNVTGGKAPSGNEVELVKEESYFFKMSKYAD
EECCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
WLLDYYQTHPNFIQPASRMNEMINNFIKPGLEDLAVTRTGFKWGVPIKNDPKHVIYVWID
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHEEECCCEECCCCCCCCCEEEEEEHH
ALSNYITALGYSSDEDGNFKKFWPANVHMVGKEIVRFHTIYWPIMLHALGLEQPDQIYGH
HHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
GWLLMKDSKMSKSKGNVIYPETIVKNYGLDALRYYLLKAMPYGNDGNFTPEDFISKINFD
EEEEEECCCCCCCCCCEECHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHH
LANDLGNLLNRTVAMINKYEGGVIPTFQSGVNDVDVDLETTAATTIKNYEDLMNNLHFAD
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHH
ALAEIWKLVARTNKYIDETTPWILARNDDDESKQKLASVMNHLAESLRVIAILIQPMMTQ
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPAKIFAQLGLDVNQLELQGLKFGEIPADTKVVEKGTPIFPRLDVEKEVEFLKNQMTQSD
CHHHHHHHHCCCCCHHEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
KKKGRAAMAEANQKKAEAVATDQKKEIRIEAFDKVELRVAEVLEAGPVKGADKLLKFKLD
HHHCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCHHEEEEEC
AGDEEPRQILSGIAQSYPDPSVLVGKKVVIVANLKPRKMRGEMSQGMILSTEHDDQVKVV
CCCCCHHHHHHHHHHCCCCCCEEECCEEEEEECCCCHHHHCHHHCCEEEEECCCCCEEEE
MVDDQHQNGDVLG
EEECCCCCCCCCC
>Mature Secondary Structure 
AENNTYYITTPIYYPSGKLHIGNSYTTIACDAEARFQRLEGKDVFFLTGTDEHGLKIEQ
CCCCEEEEECCEEECCCEEEECCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCEEHH
KAEALNTTPKAYVDGMAEDIKKLWDLLEITNDKFIRTTDDYHEKAVQKIFQKLLDQGDIY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCEE
LGEYEGWYSVSDEEYFTESQLAEVYRDADGNVTGGKAPSGNEVELVKEESYFFKMSKYAD
EECCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
WLLDYYQTHPNFIQPASRMNEMINNFIKPGLEDLAVTRTGFKWGVPIKNDPKHVIYVWID
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHEEECCCEECCCCCCCCCEEEEEEHH
ALSNYITALGYSSDEDGNFKKFWPANVHMVGKEIVRFHTIYWPIMLHALGLEQPDQIYGH
HHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
GWLLMKDSKMSKSKGNVIYPETIVKNYGLDALRYYLLKAMPYGNDGNFTPEDFISKINFD
EEEEEECCCCCCCCCCEECHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHH
LANDLGNLLNRTVAMINKYEGGVIPTFQSGVNDVDVDLETTAATTIKNYEDLMNNLHFAD
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHH
ALAEIWKLVARTNKYIDETTPWILARNDDDESKQKLASVMNHLAESLRVIAILIQPMMTQ
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPAKIFAQLGLDVNQLELQGLKFGEIPADTKVVEKGTPIFPRLDVEKEVEFLKNQMTQSD
CHHHHHHHHCCCCCHHEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
KKKGRAAMAEANQKKAEAVATDQKKEIRIEAFDKVELRVAEVLEAGPVKGADKLLKFKLD
HHHCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCHHEEEEEC
AGDEEPRQILSGIAQSYPDPSVLVGKKVVIVANLKPRKMRGEMSQGMILSTEHDDQVKVV
CCCCCHHHHHHHHHHCCCCCCEEECCEEEEEECCCCHHHHCHHHCCEEEEECCCCCEEEE
MVDDQHQNGDVLG
EEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]