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The map label for this gene is pdhD [H]

Identifier: 116492048

GI number: 116492048

Start: 288364

End: 289698

Strand: Direct

Name: pdhD [H]

Synonym: PEPE_0244

Alternate gene names: 116492048

Gene position: 288364-289698 (Clockwise)

Preceding gene: 116492047

Following gene: 116492049

Centisome position: 15.74

GC content: 39.03

Gene sequence:

>1335_bases
ATGAAAAAGTATGACGTTGTGATTATTGGAGCAGGACCTGGTGGATTAGGATTAGCTTATCCTTTAAAAGAAGCGGGGTT
GGAAGTGGCAGTTGTTGAAGAAAATCTTTGGGGCGGAACGTGTCCTAATCGAGGATGTGACCCTAAAAAGGTTCTATTAG
CAGCGATTGAAGCTAAAAAACAAAATCAATATTTGCTTGGTAATGGAATTAAAAATGAGACTCAAATTGATTGGCCTGCT
TTAATGCAATTTGAAAAAACATTTACAGATCCAGTTTCTAGAAGCAGCCGTTCAGGTTTAACGGACGCTCAAATCGATGT
TTATGATGGACATGCTGAATTTATTGACCACCACACCTTGAAGATCGATCAAACAACCATCACCAGTGAAAAATTTGTGA
TTGCAACGGGACAACGCCCTAGTCGATTAACAAATATTGAAAATCAAGAGATGATGCAGACTAGTACAGATTTCTTAAAG
ATGGATCATCTACCAAATGAACTGGCCTTGGTCGGTGGAGGATATATAGCATTTGAATTGGCGATGATTGCTAGTGGCGC
AGGGGCTAAGGTACACATTATTCATCATAATCAACGCCCATTAAAAGCTTTTCCTAAAGCATATGTCGATGACTTAGTCG
ATCAACTTAAAACTCAAGGAGTTGAAGTTCACTTAGGAATTGATTTAAAGCGGGTAGAACCTAAGGATGAACGGTTTGAG
TTGAGTGATGGACAAGGTTTCAAGCAAATCGTTGATCAGGTTTTTGTTACAGCTGGGAGAAAACCCAATGATGATAGTTT
AAATCTAGAAAAGATTGGTGTACAAACTGATCGTGGTGGAATCGTAGTTAATGAATATTTGGAAACATCGGTTGATAATA
TCTATGCGATGGGGGACGTTGTTTCCAAGGCGCTTCCTAAACTGACTCCAGTATCTGGATTTGAAGCCCGTTATTTGGCT
AAAAAGCTAACGAATGCAACCCAAGCAGCGATTGATTATCCAGCAATCCCTACCGTAGTATACGGAATGCCTAAATTGGC
TAAGGTAGGAGTTGAATTGGATGAGGCGCAGCAACATCCAGAACAATATGAAATAAAGACAGCGGAGATGACAGAGTGGT
TTACTTATCGGCGTTTACAAGATCCGTTCGCTAAAATTAGTGTTATTCATGAAAAGTCCAGTGGAAAAATTGTTGGTGCA
ATTACAATGAGTAGTGAAGCAGAACATCTAATTAATGATTTAACGATGATTATTAATCAAGGGGGATTAGTAGATCAAAT
CAACAATCAAATCTTAGCTTATCCTACGGAAGCCAGTGATTTAGGCTATATGTAG

Upstream 100 bases:

>100_bases
TAAAAGTAGTAGATGAAGATAAATCAACGGTTAATTGGGTTGACCGAGCATCATTATTTAGGTATTTAAGTGAAACCAAA
CATTAAGGGAGGTAAATTAA

Downstream 100 bases:

>100_bases
TATACTTAATTAGTGATTGTTATAATTTAGCTTTATTTTGAAAGGAACGTGGTTTAATGGAGTTCGTGGGCGTACTTGTA
CTAATTTTAGTACTGACCCT

Product: pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, related enzyme

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKKYDVVIIGAGPGGLGLAYPLKEAGLEVAVVEENLWGGTCPNRGCDPKKVLLAAIEAKKQNQYLLGNGIKNETQIDWPA
LMQFEKTFTDPVSRSSRSGLTDAQIDVYDGHAEFIDHHTLKIDQTTITSEKFVIATGQRPSRLTNIENQEMMQTSTDFLK
MDHLPNELALVGGGYIAFELAMIASGAGAKVHIIHHNQRPLKAFPKAYVDDLVDQLKTQGVEVHLGIDLKRVEPKDERFE
LSDGQGFKQIVDQVFVTAGRKPNDDSLNLEKIGVQTDRGGIVVNEYLETSVDNIYAMGDVVSKALPKLTPVSGFEARYLA
KKLTNATQAAIDYPAIPTVVYGMPKLAKVGVELDEAQQHPEQYEIKTAEMTEWFTYRRLQDPFAKISVIHEKSSGKIVGA
ITMSSEAEHLINDLTMIINQGGLVDQINNQILAYPTEASDLGYM

Sequences:

>Translated_444_residues
MKKYDVVIIGAGPGGLGLAYPLKEAGLEVAVVEENLWGGTCPNRGCDPKKVLLAAIEAKKQNQYLLGNGIKNETQIDWPA
LMQFEKTFTDPVSRSSRSGLTDAQIDVYDGHAEFIDHHTLKIDQTTITSEKFVIATGQRPSRLTNIENQEMMQTSTDFLK
MDHLPNELALVGGGYIAFELAMIASGAGAKVHIIHHNQRPLKAFPKAYVDDLVDQLKTQGVEVHLGIDLKRVEPKDERFE
LSDGQGFKQIVDQVFVTAGRKPNDDSLNLEKIGVQTDRGGIVVNEYLETSVDNIYAMGDVVSKALPKLTPVSGFEARYLA
KKLTNATQAAIDYPAIPTVVYGMPKLAKVGVELDEAQQHPEQYEIKTAEMTEWFTYRRLQDPFAKISVIHEKSSGKIVGA
ITMSSEAEHLINDLTMIINQGGLVDQINNQILAYPTEASDLGYM
>Mature_444_residues
MKKYDVVIIGAGPGGLGLAYPLKEAGLEVAVVEENLWGGTCPNRGCDPKKVLLAAIEAKKQNQYLLGNGIKNETQIDWPA
LMQFEKTFTDPVSRSSRSGLTDAQIDVYDGHAEFIDHHTLKIDQTTITSEKFVIATGQRPSRLTNIENQEMMQTSTDFLK
MDHLPNELALVGGGYIAFELAMIASGAGAKVHIIHHNQRPLKAFPKAYVDDLVDQLKTQGVEVHLGIDLKRVEPKDERFE
LSDGQGFKQIVDQVFVTAGRKPNDDSLNLEKIGVQTDRGGIVVNEYLETSVDNIYAMGDVVSKALPKLTPVSGFEARYLA
KKLTNATQAAIDYPAIPTVVYGMPKLAKVGVELDEAQQHPEQYEIKTAEMTEWFTYRRLQDPFAKISVIHEKSSGKIVGA
ITMSSEAEHLINDLTMIINQGGLVDQINNQILAYPTEASDLGYM

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=29.4117647058824, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI22035672, Length=425, Percent_Identity=26.3529411764706, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=24.6696035242291, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI291045266, Length=337, Percent_Identity=26.1127596439169, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519430, Length=425, Percent_Identity=23.0588235294118, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI33519428, Length=425, Percent_Identity=23.0588235294118, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI33519426, Length=425, Percent_Identity=23.0588235294118, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI148277065, Length=425, Percent_Identity=23.0588235294118, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI148277071, Length=425, Percent_Identity=23.0588235294118, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI291045268, Length=308, Percent_Identity=26.2987012987013, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1789915, Length=429, Percent_Identity=30.5361305361305, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082354, Length=448, Percent_Identity=27.6785714285714, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=27.9379157427938, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786307, Length=457, Percent_Identity=26.9146608315098, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=30.1486199575372, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71983429, Length=438, Percent_Identity=26.027397260274, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=438, Percent_Identity=26.027397260274, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=451, Percent_Identity=23.5033259423503, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=347, Percent_Identity=24.207492795389, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=25.3763440860215, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321091, Length=468, Percent_Identity=23.5042735042735, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325240, Length=467, Percent_Identity=23.1263383297645, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=26.8817204301075, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640553, Length=465, Percent_Identity=26.8817204301075, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=26.8817204301075, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=25.7019438444924, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=27.0042194092827, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48945; Mature: 48945

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYDVVIIGAGPGGLGLAYPLKEAGLEVAVVEENLWGGTCPNRGCDPKKVLLAAIEAKK
CCCEEEEEEECCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
QNQYLLGNGIKNETQIDWPALMQFEKTFTDPVSRSSRSGLTDAQIDVYDGHAEFIDHHTL
CCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEECCHHHHHCCCEE
KIDQTTITSEKFVIATGQRPSRLTNIENQEMMQTSTDFLKMDHLPNELALVGGGYIAFEL
EEECEEECCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
AMIASGAGAKVHIIHHNQRPLKAFPKAYVDDLVDQLKTQGVEVHLGIDLKRVEPKDERFE
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCEE
LSDGQGFKQIVDQVFVTAGRKPNDDSLNLEKIGVQTDRGGIVVNEYLETSVDNIYAMGDV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECEEECCCCEEEHHHHHHHHHHHHHHHHH
VSKALPKLTPVSGFEARYLAKKLTNATQAAIDYPAIPTVVYGMPKLAKVGVELDEAQQHP
HHHHHCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCHHHHCCCHHHHHCCCHHHHHCCC
EQYEIKTAEMTEWFTYRRLQDPFAKISVIHEKSSGKIVGAITMSSEAEHLINDLTMIINQ
HHEEEEHHHHHHHHHHHHHHCCHHEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCC
GGLVDQINNQILAYPTEASDLGYM
CCCHHHHCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKKYDVVIIGAGPGGLGLAYPLKEAGLEVAVVEENLWGGTCPNRGCDPKKVLLAAIEAKK
CCCEEEEEEECCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
QNQYLLGNGIKNETQIDWPALMQFEKTFTDPVSRSSRSGLTDAQIDVYDGHAEFIDHHTL
CCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEECCHHHHHCCCEE
KIDQTTITSEKFVIATGQRPSRLTNIENQEMMQTSTDFLKMDHLPNELALVGGGYIAFEL
EEECEEECCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
AMIASGAGAKVHIIHHNQRPLKAFPKAYVDDLVDQLKTQGVEVHLGIDLKRVEPKDERFE
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCEE
LSDGQGFKQIVDQVFVTAGRKPNDDSLNLEKIGVQTDRGGIVVNEYLETSVDNIYAMGDV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECEEECCCCEEEHHHHHHHHHHHHHHHHH
VSKALPKLTPVSGFEARYLAKKLTNATQAAIDYPAIPTVVYGMPKLAKVGVELDEAQQHP
HHHHHCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCHHHHCCCHHHHHCCCHHHHHCCC
EQYEIKTAEMTEWFTYRRLQDPFAKISVIHEKSSGKIVGAITMSSEAEHLINDLTMIINQ
HHEEEEHHHHHHHHHHHHHHCCHHEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCC
GGLVDQINNQILAYPTEASDLGYM
CCCHHHHCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]